LOCUS       sequence-422352-        8431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human eGFP-SUN-Delta-N in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Shokrollahi M, Stanic M, Hundal A, Chan JNY, Urman D, Hakem A, 
            Garcia RE, Hao J, Maass PG, Dickson BC, Hande MP, Pujana MA, Hakem 
            R, Mekhail K
  TITLE     DNA double-strand break-capturing nuclear envelope tubules drive DNA
            repair
  JOURNAL   bioRxiv 2023.05.07.539750
REFERENCE   2  (bases 1 to 8431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.05.07.539750"
COMMENT     Sequence Label: eGFP-SUN1-Delta-N
FEATURES             Location/Qualifiers
     source          1..8431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             964..984
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1000..1002,1003..1005,1006..1716)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1000..1002,1003..1005,1006..1716)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1045..1066)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1306..1325)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1653..1674
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4022..4039)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4028..4252
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4298..4726
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4385..4404)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4595..4616
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4735..4755)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4740..5069
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4920..5055
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4982..5001
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5136..5930
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5190..5209)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5800..5819
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6104..6225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6141..6160)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6195..6214
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6274..6290)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6274..6290)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6287..6309)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6298..6314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6322..6328,6329..6346,6347..6352))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6367..6388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6505..6522)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6676..7264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6756..6775)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7435..8226,8227..8295))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8058..8077
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8296..8400)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgc ttaagggagg tggatctgga ggtggatctg gaggtggatc tggaggtgga
      961 tctcccaaga agaagaggaa ggtgggaggt tctaccggta tggtgagcaa gggcgaggag
     1021 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1081 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1141 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1201 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1261 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1321 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1381 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1441 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1501 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1561 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     1621 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1681 gccgggatca ctctcggcat ggacgagctg tacaagggag gttctgttaa ctggattcgt
     1741 gaacagacca cagtggacca cttctggggt cttgatgatg atggtgatct taaaggtgga
     1801 aataaagctg ccattcaggg aaacggggat gtgggagccg ccgccgccac cgcgcacaac
     1861 ggcttctcct gcagcaactg cagcatgctg tccgagcgca aggacgtgct cacggcgcac
     1921 cccgcggccc ccgggcccgt gtcgagagtt tattctaggg acaggaatca aaaatgcggt
     1981 gcgtctttct acgtgaatag gattttgtgg ctggccagat acactgcatc atctttttca
     2041 tcatttttag ttcaactttt tcaagtggtt ttaatgaagc tcagttatga atcagaaaat
     2101 tacaaattga aaactcatga atcaaaagat tgtgaatccg aaagctataa gtcaaaaagc
     2161 catgaatcaa aaggcgacga ctgtaagggc aagaggcacc tcgacgcgca cacagccgcc
     2221 cactcgcagt cgccacggct gcccggtcgg gcagggaccc tctggcacat ctgggcatgt
     2281 gcaggttact tcttgctgca gattctgcgc aggatcggag ctgtgggcca ggctgtgtcc
     2341 aggacggcgt ggtcggccct ttggctggcc gtggttgctc cagggaaggc agcctctgga
     2401 gtgttctggt ggctggggat tggatggtac cagtttgtta ctttgatttc ttggctgaat
     2461 gtgtttcttc ttaccaggtg ccttcgaaac atctgcaagt ttttagtctt gctcatccca
     2521 ctcttccttt tactagcagg tctctcctta cggggccagg gcaatttctt ttcgttcttg
     2581 cccgtgttga attgggcaag catgcataga acacagcggg tggatgaccc ccaggacgtg
     2641 tttaaaccca cgacttctcg cctgaagcag cctctgcagg gtgacagtga ggcttttccg
     2701 tggcattgga tgagtggcgt ggagcagcag gtggcctctc tgtctggaca gtgccaccac
     2761 catggtgaga atctccgaga gctgaccact ttgctacaga agctgcaggc tcgggtggac
     2821 cagatggaag gcggcgctgc cgggccgtca gcttcggtca gagacgctgt gggacagccc
     2881 ccgagggaga ctgactttat ggcctttcac caagaacatg aagtgcgtat gtcacacttg
     2941 gaagatattc tgggaaaact gagagaaaaa tctgaggcca tccagaagga actagaacag
     3001 accaagcaaa aaacaatcag tgcggttggt gagcagctcc tgcccacggt cgagcacctc
     3061 cagctggagc tggatcagct aaagtcagag ctgtccagct ggcgacacgt gaagaccggc
     3121 tgtgagacag tggatgccgt acaagaaaga gtggacgtgc aagtcagaga aatggtgaaa
     3181 ctcctgtttt ccgaagatca gcaaggcggt tctctggaac agctgctgca gaggttctca
     3241 tcacagtttg tgagcaaagg cgacttgcag acgatgctgc gagacctgca gctgcagatc
     3301 ctgcggaacg tcacccacca cgtttccgtg accaagcagc tcccaacctc agaagccgtg
     3361 gtgtctgctg tgagcgaggc gggggcgtct ggaataacag aggcgcaagc acgtgccatc
     3421 gtgaacagcg ccttgaagct gtattcccaa gataagaccg ggatggtgga ctttgctctg
     3481 gaatctggtg gtggcagcat cttgagtact cgctgttctg aaacttacga aaccaaaacg
     3541 gcgctgatga gtctgtttgg gatcccgctg tggtacttct cgcagtcccc gcgcgtggtc
     3601 atccagcctg acatttaccc cggtaactgc tgggcattta aaggctccca ggggtacctg
     3661 gtggtgaggc tctccatgat gatccaccca gccgccttca ctctggagca catccctaag
     3721 acgctgtcgc caacaggcaa catcagcagc gcccccaagg acttcgccgt ctatggatta
     3781 gaaaatgagt atcaggaaga agggcagctt ctgggacagt tcacgtatga tcaggatggg
     3841 gagtcgctcc agatgttcca ggccctgaaa agacccgacg acacagcttt ccaaatagtg
     3901 gaacttcgga ttttttctaa ctggggccat cctgagtata cctgtctgta tcggttcaga
     3961 gttcatggcg aacctgtcaa gtgactcgag tctagagggc ccgtttaaac ccgctgatca
     4021 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     4081 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     4141 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     4201 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     4261 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     4321 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4381 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4441 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4501 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4561 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4621 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4681 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     4741 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     4801 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     4861 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     4921 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     4981 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     5041 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     5101 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
     5161 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     5221 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
     5281 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
     5341 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     5401 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     5461 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     5521 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     5581 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     5641 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg
     5701 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     5761 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     5821 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     5881 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     5941 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     6001 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6061 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     6121 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6181 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     6241 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6301 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6361 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7321 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7381 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7441 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7501 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7561 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7621 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7681 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7741 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7801 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7861 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7921 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7981 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8041 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8101 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8161 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8221 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8281 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8341 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8401 cacatttccc cgaaaagtgc cacctgacgt c
//
