LOCUS       sequence-422358-        2878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BTK Type 1 homology arm for Golden Gate assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Lariviere PJ, Zuberi Ashraf AHM, Leonard SP, Miller LG, Moran NA, 
            Barrick JE
  TITLE     Single-step genome engineering in the bee gut symbiont Snodgrassella
            alvi
  JOURNAL   bioRxiv 2023.09.19.558440
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023.09.19.558440"
COMMENT     Sequence Label: pBTK1050
FEATURES             Location/Qualifiers
     source          1..2878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     195..214
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      462..1050
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     951..970
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(2394..2428)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(join(2499..2878,1..280))
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
        1 cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac
       61 gaaattccgg atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact
      121 tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt
      181 tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg
      241 atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg
      301 aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta tggtgaaagt
      361 tggaacctct tacgtgcccg atcaatcatg accaaaatcc cttaacgtga gttttcgttc
      421 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
      481 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
      541 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
      601 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
      661 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
      721 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
      781 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
      841 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
      901 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
      961 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     1021 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     1081 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     1141 gataaccgta cgtttgagaa gagaaaagaa aaccgccgat cctgtccacc gcattactgc
     1201 aaggtagtgg acaagaccgg cggtcttaag ttttttggct gaaagtcggt ctcaccctga
     1261 attcaacctg ccctataccg tatggttttt attcatggca ggctgtctgt ccctgtttct
     1321 gtgtatttct tatatctatt accgcaacaa attcataaca acctacgcat acctacccta
     1381 cggacgtgcc agcaacgccg accagcttcc caacgtcaac tggataaaat taaccagccc
     1441 ctgcctgcct gaaacctctg tcattaatgg cattgttgcc gacctgcacg aacctgagct
     1501 cgacaaccac tggcagaaat ttcttgcccg gcaaaccctc aatggcatgc ctgtctacaa
     1561 tattcgtcag gtagaagaat ttcttaccgg acgggttaaa attcatcacc tatatgaaaa
     1621 tgatcttggt tctttgctgc cttcaccaac ctatgtattg attaaacgcg tattcgacat
     1681 cgttcttatc ctgctatccg cccctgttac cctgccggta atgcttatta ctgccattgc
     1741 catcaaactt gaaagcccgg gtaacatcct gttccggcaa aaccgtgtag gacgcagcgg
     1801 caaagaattc tgtatttata aatttcgcag catgtgcagc gattcagaga aaaacggtgc
     1861 ccgcatggcg caacttgggg atgcccgcgt cacccgtatc ggacacatca tccgcaaaac
     1921 ccgcatcgac gagctgccgc agttctggaa catcctgaaa ggtgacatga gcctgattgg
     1981 tccccgccct gagcaaaaag tatttgtcga ccagtttatt caggaaattc ccttttatga
     2041 ctaccgccat attgtcaaac ccggtatttc cggctgggcg caagttactc agggctacgc
     2101 tgccgacacc gaacaaaccc agataaaaat cgaacatgat ttttactaca tcaaacattt
     2161 ttccttctca ctcgacatac tcatattctt caaaaccctc aaaaccatgc tcaccggctt
     2221 cggcgcacgc taacccagcc atccccatac aaaacgctac cagtaaaatc ttaccggtag
     2281 cgttgcaata cacttacaaa aaccacatag cacaactgcc gaacgtgaga ccggaaacac
     2341 agaaaaaagc ccacacctga cagtgcgggc tttttttttc gaccaaagga ccaataaaaa
     2401 acgcccggcg gcaaccgagc gttctgaaca aatccagatg gagttctgag gtcattactg
     2461 gatctatcaa caggagtcca agcgagctcg atatcaaatt acgccccgcc ctgccactca
     2521 tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca
     2581 tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc
     2641 atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg
     2701 aaactcaccc agggattggc tgaaacgaaa aacatattct caataaaccc tttagggaaa
     2761 taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg
     2821 aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaa
//
