LOCUS       sequence-422374-        7584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7584)
  TITLE     Patrick Moore plasmids
REFERENCE   2  (bases 1 to 7584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA6.MCV.cLT206.V5(CM2B4)
FEATURES             Location/Qualifiers
     source          1..7584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        151..530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        531..734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     685..705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     779..798
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        779..797
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3365..3406
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3416..3433
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3456..3473)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3462..3686
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3732..4160
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3819..3838)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4029..4050
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4169..4189)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4174..4503
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4354..4489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4416..4435
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4551..4598
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4617..5015
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5173..5294
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5210..5229)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5264..5283
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5343..5359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5343..5359)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5356..5378)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5367..5383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5391..5397,5398..5415,5416..5421))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5436..5457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5574..5591)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5745..6333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5825..5844)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6504..7295,7296..7364))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7127..7146
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7365..7469)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7544..7563)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
       61 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
      121 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
      181 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      241 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      301 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      361 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
      421 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
      481 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      541 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      601 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      661 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      721 atataagcag agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta
      781 atacgactca ctatagggag acccaagctg gctagcgttt aaacttaagc ttggtaccga
      841 gctcggatcc actagtccag tgtggtggaa ttctgcagat atcatggatt tagtcctaaa
      901 taggaaagaa agagaggctc tctgcaagct tttagagatt gctcctaatt gttatggcaa
      961 catccctctg atgaaagctg ctttcaaaag aagctgctta aagcatcacc ctgataaagg
     1021 gggaaatcct gttataatga tggaattgaa caccctttgg agcaaattcc agcaaaatat
     1081 ccacaagctc agaagtgact tctctatgtt tgatgaggtt gacgaggccc ctatatatgg
     1141 gaccactaaa ttcaaagaat ggtggagatc aggaggattc agcttcggga aggcatatga
     1201 atatgggccc aatccacacg ggaccaactc aagatccaga aagccttcct ccaatgcatc
     1261 caggggagcc cccagtggaa gctcaccacc ccacagccag agctcttcct ctgggtatgg
     1321 gtccttctca gcgtcccagg cttcagaccc ccagtccaga ggacccgata tacctcccga
     1381 acaccatgag gaacccacct catcctctgg atccagtagc agagaggaga ccaccaattc
     1441 aggaagagaa tccagcacac ccaatggaac cagtgtacct agaaattctt ccagaacgga
     1501 tggcacctgg gaggatctct tctgcgatga atcactttcc tcccctgagc ctccctcgtc
     1561 ctctgaggag cctgaggagc ccccctcctc aagaagctcg ccccggcagc ccccgtcttc
     1621 ctctgccgag gaggcctcgt catctcagtt tacagatgag gaatacagat cctcctcctt
     1681 caccaccccg aagacccctc ctccattctc aagaaagcga aaatttgggg ggtcccgaag
     1741 ctctgcaagc tctgctagtt cagcaagttt tacaagcact ccaccaaagc caaaaaagaa
     1801 cagagaaact cctgttccta ctgattttcc tattgatctt tctgattatc ttagccatgc
     1861 tgtatatagt aataaaacag taagttgttt tgccatttat actacttctg ataaagctat
     1921 agagttatat gataagattg agaaatttaa agttgatttt aaaagcaggc atgcctgtga
     1981 attaggatgt attttattgt ttataacttt atcaaagcat agagtatctg ctattaagaa
     2041 tttttgctct accttctgca ctataagctt tttaatttgt aaaggagtga ataagatgcc
     2101 tgaaatgtat aataatttat gcaagccccc ttacaaatta ctgcaagaga ataagccact
     2161 gctaaattat gaatttcaag aaaaagaaaa agaggccagc tgtaattgga atttagttgc
     2221 tgaatttgct tgtgaatatg agctagacga ccactttatt atcttagccc attatctaga
     2281 ctttgcaaaa ccatttcctt gccaaaagtg tgaaaacaga tctcgcctca aacctcacaa
     2341 ggctcatgag gctcatcatt ctaatgctaa gctattttat gaatctaaat ctcagaaaac
     2401 catttgccaa caagccgcag acactgttct agccaaaagg aggttagaga tgctggaaat
     2461 gaccaggaca gaaatgctat gtaagaagtt taagaagcac ctagagagat taagagattt
     2521 agatacaata gatctactgt attatatggg tggtgtggcc tggtactgct gcttatttga
     2581 agagtttgaa aagaagctgc agaaaattat tcaattatta acagagaata tacctaagta
     2641 tagaaacatt tggtttaaag ggcctattaa cagtggaaaa acaagctttg ctgcagcctt
     2701 aatagatttg ctagaaggga aggccttgaa tataaactgt ccatctgata agctgccttt
     2761 tgaactagga tgtgctttgg ataaatttat ggttgttttt gaggatgtga aagggcaaaa
     2821 tagcctaaat aaagatctgc aaccagggca aggaataaat aaccttgata acttaagaga
     2881 tcatctagat ggtgctgtag ctgtaagctt agagaagaag catgtgaata aaaagcatca
     2941 gatttttcct ccttgtattg ttactgctaa tgattatttt attcccaaaa cattaatagc
     3001 aagatttagt tatactttac acttttcccc aaaggcaaat ctaagagatt ccctggatca
     3061 gaacatggaa ataagaaaaa gaagaattct tcaaagtgga accactttat tgctttgtct
     3121 tatttggtgc ttgcctgata caacctttaa gccttgctta caagaagaaa ttaaaaactg
     3181 gaagcaaatt ttacagagtg aaatatcata tggtaaattt tgtcaaatga tagaaaatgt
     3241 agaagctggt caggaccctc tgctcaatat tcttattgag gaagagggcc ctgaggaaac
     3301 tgaagaaacc caagattctg gtactttttc tcaatactcg agtctagagg gcccgcggtt
     3361 cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca
     3421 tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     3481 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     3541 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     3601 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     3661 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     3721 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     3781 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     3841 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     3901 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     3961 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4021 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4081 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4141 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     4201 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     4261 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     4321 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4381 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4441 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     4501 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt
     4561 aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg
     4621 ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca
     4681 gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca
     4741 tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc
     4801 tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg
     4861 agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc
     4921 ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg
     4981 aattgctgcc ctctggttat gtgtgggagg gctaagcact tcgtggccga ggagcaggac
     5041 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     5101 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     5161 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5221 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5281 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     5341 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     5401 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     5461 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     5521 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5581 actgactcgc tgcgctcggt ctttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5641 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5701 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5761 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5821 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5881 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5941 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     6001 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     6061 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     6121 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6181 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6241 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6301 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6361 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6421 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6481 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6541 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6601 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6661 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6721 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6781 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6841 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6901 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6961 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     7021 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     7081 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     7141 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7201 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7261 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7321 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7381 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7441 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     7501 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
     7561 ccgcatagtt aagccagtat ctgc
//
