LOCUS       sequence-42238-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Kadener S, Menet JS, Sugino K, Horwich MD, Weissbein U, Nawathean P,
            Vagin VV, Zamore PD, Nelson SB, Rosbash M
  TITLE     A role for microRNAs in the Drosophila circadian clock.
  JOURNAL   Genes Dev. 2009 Sep 15;23(18):2179-91. Epub 2009 Aug 20.
  PUBMED    19696147
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Dev."; date: "2009-09-15"; volume: "23(18)"; pages: "2179-91. Epub 
            2009 Aug 20"
COMMENT     Sequence Label: pAC-luc-CLK
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             653..694
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             704..721
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(744..761)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 tccgaaggtc ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc
       61 aagaagggcg gaaagtccaa attgtaaccg cgggcgcgcg ctagcgcggc cgcgtagtca
      121 ataggtatac ccactgtata taacactctg tagcgatagg aaaagataga attgtataac
      181 tccaggcaac ccatataacc acatatccgt gtattatcaa gtttggcctc tggctctctt
      241 tatcgaacct catctatcgg aagcccagag ctagttgtag atctcaacag actgagtatt
      301 gtcccacccc gctcccatta tgcgttgtat ataacataaa cataggtgta ctatagaaaa
      361 ggtctcaaaa aaaaaaaatc cattgtaagt aggttctaaa ttgttgtagt ttgtagcaag
      421 aacccatcgt ttccataggt tagccttatc cagtaagtgc catcgaggcg caaacaagga
      481 tctctgatac gaaatcgaac cttaaaacct aatcctaagt tatttgtatt ctaatatact
      541 gagaagcgac taacaagcaa acgagttgag caaagctaat tcaattccat attattttca
      601 agactgggct tcgtcattga ggtcctcgag tctagagggc ccgcggttcg aaggtaagcc
      661 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca
      721 ttgagtttaa acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc
      781 agcctcgatc gaggatccag acatgataag atacattgat gagtttggac
//
