LOCUS       sequence-422490-        2780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part of Bactoneuron DF4, expresses mTagBFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Srivastava R, Bagh S
  TITLE     A Logically Reversible Double Feynman Gate with Molecular Engineered
            Bacteria Arranged in an Artificial Neural Network-Type Architecture.
  JOURNAL   ACS Synth Biol. 2023 Jan 20;12(1):51-60. doi: 
            10.1021/acssynbio.2c00520. Epub 2022 Nov 16.
  PUBMED    36384003
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00520"; journalName: "ACS Synth Biol"; date: 
            "2023-01-20- 20"; volume: "12"; issue: "1"; pages: "51-60"
COMMENT     Sequence Label: pHTC_O3
FEATURES             Location/Qualifiers
     source          1..2780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             608..619
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(793..811)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        877..979
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             980..1639
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1046..1065)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1660..1754
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      1868..2413
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     2314..2333
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2530..2547
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2582..2668)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(join(2680..2780,1..601))
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
ORIGIN
        1 tttccagtct gtagtccaca tagtagacgc caggcatctt gaggttctta gcgggtttct
       61 tggatctata tgtggtcttg gcgtttgcga tcagatggct cccgcccacg agcttcaggg
      121 ccatgtcgtt tctgccttcc aggccgccgt cagcggggta cagcgtctcg gtgaaggcct
      181 cccagccgag tgttttcttc tgcatcacag ggccgttgga tgtgaagttc acccctctga
      241 tcttgacgtt gtagatgagg cagccgtcct ggaggctggt gtcctgggta gcggtcagca
      301 cgcccccgtc ttcgtatgtg gtgactctct cccatgtgaa gccctcaggg aaggactgct
      361 tgaagaagtc ggggatgccc tgggtgtggt tgatgaaggt cttgctgccg tagaggaagc
      421 tagtagccag gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc ttgattctca
      481 tggtctgggt gccctcgtag ggcttgcctt cgccctcgga tgtgcacttg aagtgatggt
      541 tgtccacggt gccctccatg tacagcttca tgtgcatgtt ctccttaatc agctcgctca
      601 tggtaccttt ctcctcttta atgaattcct ctatcactga tagggaatca tcctctatca
      661 ctgataggga atcatctatc accgccagag gtaatcatct atcaccgcca gaggtattta
      721 ttcgactata tatcaccgcc agaggtacgt aaacctgtac gatcctacag gtctcgaggt
      781 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      841 tttcttagac gtcgatatct ggcgaaaatg agacgttgat cggcacgtaa gaggttccaa
      901 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
      961 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
     1021 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
     1081 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     1141 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
     1201 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     1261 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     1321 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     1381 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     1441 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     1501 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     1561 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     1621 gtggcagggc ggggcgtaat ttgatatcga gctcgcttgg actcctgttg atagatccag
     1681 taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt
     1741 ttattggtga gaatccaagc actagtaaca acttatatcg tatggggctg acttcaggtg
     1801 ctacatttga agagataaat tgcactgaaa tctagaaata ttttatctga ttaataagat
     1861 gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac gaaaaaaccg
     1921 ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac cgaggtaact
     1981 ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa ccggcgcatg
     2041 acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg tgcttttgca
     2101 tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcggactga
     2161 acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga actgagtgtc
     2221 aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa accgaaaggc
     2281 aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc tttatagtcc
     2341 tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt caggggggcg
     2401 gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag tatcttcctg
     2461 gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg cagtcgaacg
     2521 accgagcgta gcgagtcagt gagcgaggaa gcggaatata tccctaggtc tagggcggcg
     2581 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
     2641 ctttcgactg agcctttcgt tttatttgat gcctctagat taattaagct tgtgccccag
     2701 tttgctaggg aggtcgcagt atctggccac tgccacctcg tgctgctcga cgtaggtctc
     2761 gttgttggcc tccttgattc
//
