LOCUS       sequence-422491-        4138 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  part of Bactoneuron DF5, expresses mTagBFP2 and LuxR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Srivastava R, Bagh S
  TITLE     A Logically Reversible Double Feynman Gate with Molecular Engineered
            Bacteria Arranged in an Artificial Neural Network-Type Architecture.
  JOURNAL   ACS Synth Biol. 2023 Jan 20;12(1):51-60. doi: 
            10.1021/acssynbio.2c00520. Epub 2022 Nov 16.
  PUBMED    36384003
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.2c00520"; journalName: "ACS Synth Biol"; date: 
            "2023-01-20- 20"; volume: "12"; issue: "1"; pages: "51-60"
COMMENT     Sequence Label: pAC_O3
FEATURES             Location/Qualifiers
     source          1..4138
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        16..50
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     RBS             60..71
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      888..974
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        1086..1190
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1191..1259,1260..2051)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1409..1428)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      2081..2175
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      2263..2851
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2752..2771
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(3124..3210)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(3222..3923)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     RBS             3930..3941
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    4029..4045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    4052..4068
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(4091..4109)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcctggtaac tcgagttgac agctagctca gtcctaggta ctgtgctagc gaattcatta
       61 aagaggagaa aggtaccatg aaaaacataa atgccgacga cacatacaga ataattaata
      121 aaattaaagc ttgtagaagc aataatgata ttaatcaatg cttatctgat atgactaaaa
      181 tggtacattg tgaatattat ttactcgcga tcatttatcc tcattctatg gttaaatctg
      241 atatttcaat cctagataat taccctaaaa aatggaggca atattatgat gacgctaatt
      301 taataaaata tgatcctata gtagattatt ctaactccaa tcattcacca attaattgga
      361 atatatttga aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa
      421 catcaggtct tatcactggg tttagtttcc ctattcatac ggctaacaat ggcttcggaa
      481 tgcttagttt tgcacattca gaaaaagaca actatataga tagtttattt ttacatgcgt
      541 gtatgaacat accattaatt gttccttctc tagttgataa ttatcgaaaa ataaatatag
      601 caaataataa atcaaacaac gatttaacca aaagagaaaa agaatgttta gcgtgggcat
      661 gcgaaggaaa aagctcttgg gatatttcaa aaatattagg ttgcagtgag cgtactgtca
      721 ctttccattt aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc caaagtattt
      781 ctaaagcaat tttaacagga gcaattgatt gcccatactt taaaaattaa taacactgat
      841 agtgctagtg tagatcacta ctagagccag gcatcatcta gaggcatcaa ataaaacgaa
      901 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
      961 tgagtaggac aaatccgccg ccctagacct aggaacaaat gcgagatctt gatgttggcg
     1021 tcttcgttgt cgactgttcg tcttcgtccc agtagacgtc aggtggcact tttcggggaa
     1081 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     1141 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1201 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1261 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1321 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1381 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     1441 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1501 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1561 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1621 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1681 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     1741 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1801 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     1861 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     1921 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1981 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     2041 agcattggta actgtcagac caagtttacg agctcgcttg gactcctgtt gatagatcca
     2101 gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt
     2161 tttattggtg agaatccaag cactagtcat gaccaaaatc ccttaacgtg agttttcgtt
     2221 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     2281 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     2341 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     2401 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     2461 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     2521 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     2581 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     2641 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     2701 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     2761 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2821 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     2881 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     2941 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacggttgt
     3001 tttggagcac ggaactgcag aatgaatagc ttctgccagg atcctgactg gcgacgtaat
     3061 ccacgaaaat ctatacatta agacgatctc tttttccgtc atcgcctagg tctagggcgg
     3121 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     3181 gtctttcgac tgagcctttc gttttatttg atgcctctag attaattaag cttgtgcccc
     3241 agtttgctag ggaggtcgca gtatctggcc actgccacct cgtgctgctc gacgtaggtc
     3301 tcgttgttgg cctccttgat tctttccagt ctgtagtcca catagtagac gccaggcatc
     3361 ttgaggttct tagcgggttt cttggatcta tatgtggtct tggcgtttgc gatcagatgg
     3421 ctcccgccca cgagcttcag ggccatgtcg tttctgcctt ccaggccgcc gtcagcgggg
     3481 tacagcgtct cggtgaaggc ctcccagccg agtgttttct tctgcatcac agggccgttg
     3541 gatgtgaagt tcacccctct gatcttgacg ttgtagatga ggcagccgtc ctggaggctg
     3601 gtgtcctggg tagcggtcag cacgcccccg tcttcgtatg tggtgactct ctcccatgtg
     3661 aagccctcag ggaaggactg cttgaagaag tcggggatgc cctgggtgtg gttgatgaag
     3721 gtcttgctgc cgtagaggaa gctagtagcc aggatgtcga aggcgaaggg gagagggccg
     3781 ccctcgacca ccttgattct catggtctgg gtgccctcgt agggcttgcc ttcgccctcg
     3841 gatgtgcact tgaagtgatg gttgtccacg gtgccctcca tgtacagctt catgtgcatg
     3901 ttctccttaa tcagctcgct catggtacct ttctcctctt taatgaattc tatcaccgcc
     3961 agaggtaatc atctatcacc gccagaggta atcatctatc accgccagag gtagatgtgc
     4021 tcagtatctt gttatccgct cacaatgtca attgttatcc gctcacaatt ctcgaggtga
     4081 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataaaga
//
