LOCUS       sequence-422503-       12076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conditionally-replicating in Pseudomonas plasmid for cytidine base 
            editing based on pS44i8GH-2; oriV(pRO1600/ColE1), xylS (cured of 
            BsaI-sites), Pm-->repA, P14b-->msfGFP; Pm-->CBE:SpRY, 
            PEM7-->non-specific sg.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12076)
  AUTHORS   Kozaeva E, Nielsen ZS, Nieto-Dominguez M, Nikel PI
  TITLE     The pAblo.pCasso self-curing vector toolset for unconstrained 
            cytidine and adenine base-editing in Gram-negative bacteria.
  JOURNAL   Nucleic Acids Res. 2024 Jan 5:gkad1236. doi: 10.1093/nar/gkad1236.
  PUBMED    38180826
REFERENCE   2  (bases 1 to 12076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2024 Jan 5:gkad1236. doi: 10.1093/nar/gkad1236."
COMMENT     Sequence Label: pCasso
FEATURES             Location/Qualifiers
     source          1..12076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            454..562
                     /note="incP origin of transfer"
     rep_origin      656..1244
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1145..1164
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1298..2131)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      2266..2617
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     terminator      complement(2690..2776)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2801..3766)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        4666..4746
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     CDS             4806..5492
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     CDS             5541..7256
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             9672..9920
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             9951..10199
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKM"
     RBS             10253..10261
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      11045..11139
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        11162..11209
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     misc_RNA        11267..11342
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(11593..12076,1..308)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
       61 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
      121 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
      181 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
      241 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
      301 gcaaataaga caattgtcct tttccgctgc ataaccctgc ttcggggtca ttatagcgat
      361 tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt
      421 agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc
      481 cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa
      541 tcctgctctg cgaggctggc cgtaggccgg ccgataatct catgaccaaa atcccttaac
      601 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
      661 attctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
      721 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
      781 gagcgcagat accaaatact gttcttctag tatagccgta gttaggccac cacttcaaga
      841 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
      901 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
      961 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1021 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1081 aggcggacag gcatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1141 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1201 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1261 ccgtgaaagg caggccggtc cgtggtggcc acggcctcta ggccagatcc agcggcatct
     1321 gggttagtcg agcgcgggcc gcttcccatg tctcaccagg gcgagcctgt ttcgcgatct
     1381 cagcatctga aatcttcccg gccttgcgct tcgctggggc cttacccacc gccttggcgg
     1441 gcttcttcgg tccaaaactg aacaacagat gtgtgacctt gcgcccggtc tttcgctgcg
     1501 cccactccac ctgtagcggg ctgtgctcgt tgatctgcgt cacggctgga tcaagcactc
     1561 gcaacttgaa gtccttgatc gagggatacc ggccttccag ttgaaaccac tttcgcagct
     1621 ggtcaatttc tatttcgcgc tggccgatgc tgtcccattg catgagcagc tcgtaaagcc
     1681 tgatcgcgtg ggtgctgtcc atcttggcca cgtcagccaa ggcgtatttg gtgaactgtt
     1741 tggtgagttc cgtcaggtac ggcagcatgt ctttggtgaa cctgagttct acacggccct
     1801 caccctcccg gtagatgatt gtttgcaccc agccggtaat catcacactc ggtcttttcc
     1861 ccttgccatt gggctcttgg gttaaccgga cttcccgccg tttcaggcgc agggccgctt
     1921 ctttgagctg gttgtaggaa gattcgatag ggacacccgc catcgtcgct atgtcctccg
     1981 ccgtcactga atacatcact tcatcggtga caggctcgct cctcttcacc tggctaatac
     2041 aggccagaac gatccgctgt tcctgaacac tgaggcgata cgcggcctcg accagggcat
     2101 tgcttttgta aaccattggg ggtgaggcca cgttcgacat atgtccgctc ccttctctga
     2161 cgccgtccac gccctaggca tggtcatgac tccattatta ttgtttctgt tgcataaagc
     2221 ctaaggggta ggccttacta gagatagcct tacgttagtt atccttgtgt ataaggggac
     2281 actgtatctg cgtcccacaa tacaacaaat ccgtcccttt acaacaacaa atccgtccct
     2341 tcttaacaac aaatccgtcc cttaatggca acaaatccgt ccctttttaa actctacagg
     2401 ccacggatta cgtggcctgt agacgtccta aaaggtttaa aagggaaaag gaagaaaagg
     2461 gtggaaacgc aaaaaacgca ccactacgtg gccccgttgg ggccgcattt gtgcccctga
     2521 aggggcgggg gaggcgtctg ggcaatcccc gttttaccag tcccctatcg ccgcctgaga
     2581 gggcgcagga agcgagtaat cagggtatcg aggcggattc acccttggcg tccaaccagc
     2641 ggcaccagcg gcgcctgaga ggggcgcgcc cagctgtcta gggcggcgga tttgtcctac
     2701 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     2761 cttttcgttt tatttgatgc ctttaattaa acgttcgtaa tcaagccact tcctttttgc
     2821 attgacgcag ggtgtcggaa ggcaactcgc cgaacgcgct cctatagttt tcagcgaagc
     2881 gtcccaaatg taagaagccg tagtctaggg ctatctcagt tatactacgc acattggcac
     2941 tgggatcgtt caagcaggcg cggatgcttt cgagcttgcg gttgcggatg tagttcttcg
     3001 gcgtggtgcc ggcgtgcttc tcgaacaaat tgtagagcga gcgtggactc atcatcgcca
     3061 gctccgctaa ccgctcaagg ctgatattcc gtttgagatt ctcctcaatg aattgaacga
     3121 ctcgctcgaa agacgggtta cctttgctga aaatttcacg gctgacattg ctgcccagca
     3181 tttcgagcag cttggaagcg atgatccccg catagtgctc ttggacccga ggcatcgact
     3241 ttgtatgttc cgcttcgtca caaactaacc cgagtagatt gataaagcca tcgagttgct
     3301 ggagattgtg tcgcgcggcg aaacggatac cctccctcgg cttgtgccaa ttgttgtcac
     3361 tgcacgcccg atcaaggacc actgagggca atttaacgat aaatttctcg caatcttctg
     3421 aataggtcag gtcggcttgg tcatccggat tgagcagcaa tagttcgccc ggcgcaaaat
     3481 agtgctcctg gccatggcca cgccacaggc aatggccttt gagtattatt tgcagatgat
     3541 aacaggtttc taatccaggc gagattaccc tcacgctacc gccgtagctg attcgacaca
     3601 gatcgaggca tccgaagatt ctgtggtgca gcctgcctgc cgggcgcccg cccttgggca
     3661 ggcgaataga gtgcgtaccg acatactggt taacataatc ggagactgca tagggctcgg
     3721 cgtggacgaa gatctgactt ttctcgttca ataagcaaaa atccatagtt cacggttctc
     3781 ttattttaat gtgggctgct tggtgtgatg tagaaaggcg ccaagtcgat gaaaatgcat
     3841 ctcgacgtga tgcgtatacg ggttaccccc attgccacgt tgcgccatcc tttttgcaat
     3901 cagtgaccac ttttccaagc aaaaataacg ccaagcagaa cgaagacgtt ctttttaaga
     3961 agcgagaaca ccagaagttc gtgctgtcgg ggcatggggc gacgaattgg cggataaagg
     4021 ggatctgctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc acaagtttta
     4081 tccggccttt attcacattc ttgcccgcct gatgaatgct catccgtaat tacgtatggc
     4141 aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca ccgttttcca
     4201 tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt tccggcagtt
     4261 tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct atttccctaa
     4321 agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt tcaccagttt
     4381 tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca tgcatgggaa
     4441 ttagcttgat ctgaccaacg accggtagcg gagctatcca acggcggtat accaggaaaa
     4501 cacacagcag gtacatcaga acagtaccat gactgaagaa caaatagttt tttcctgatc
     4561 cataaagcag aacggcctgc tccatgacaa atctggctcc ccaactaatg ccccatgcag
     4621 ccagcataac cagcataaag tgcagtgtcc ggtttgatag ggataagtcc agccttgcaa
     4681 gaagcggata caggagtgca aaaaatggct atctctagta aggcctaccc cttaggcttt
     4741 atgcaacaga aacaataata atggagtcat gaccatgcct aggccgcggt taggaggaaa
     4801 aacatatgag tagcgaaacc ggtccggttg cagtggatcc gaccctgcgc cgtcgcattg
     4861 aaccgcacga gtttgaagtg ttctttgatc cgcgcgagct gcgcaaagaa acttgcctgc
     4921 tgtacgagat taactggggt ggccgccata gcatctggcg ccataccagc cagaacacca
     4981 ataagcacgt ggaagtgaat tttattgaaa aatttaccac cgagcgctac ttctgcccta
     5041 atacccgctg cagcatcacc tggtttctga gctggagccc gtgcggcgaa tgtagtcgcg
     5101 ccattaccga gttcctgagc cgctatccgc atgtgaccct gttcatctac atcgcccgtc
     5161 tgtaccatca cgccgatccg cgcaatcgcc aaggtctgcg tgatctgatt agcagcggtg
     5221 tgaccatcca gatcatgacc gaacaagaga gcggctattg ctggcgcaac ttcgtgaact
     5281 attctccgag caacgaagcc cactggccgc gttatccgca tctgtgggtg cgcctgtatg
     5341 tgctggagct gtactgcatc atcctgggcc tgccgccttg cctgaatatt ctgcgccgta
     5401 aacagccgca actgacattc ttcaccatcg cactgcagag ctgccattat cagcgcctgc
     5461 cgccgcacat tttatgggcc accggtctga aaagcggtag tgaaactccg ggcacaagcg
     5521 aaagcgcaac cccggaaagt gacaagaagt acagcatcgg cctggccatc ggcaccaact
     5581 ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc
     5641 tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg ctgttcgaca
     5701 gcggcgaaac agccgagaga acccggctga agagaaccgc cagaagaaga tacaccagac
     5761 ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg
     5821 acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag aagcacgagc
     5881 ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca
     5941 tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggctgacctg cggctgatct
     6001 atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag ggcgacctga
     6061 accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc tacaaccagc
     6121 tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc ctgtctgcca
     6181 gactgagcaa gagcagacgg ctggaaaatc tgatcgccca gctgcccggc gagaagaaga
     6241 atggcctgtt cggaaacctg attgccctga gcctgggcct gacccccaac ttcaagagca
     6301 acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac gacgacgacc
     6361 tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg gccgccaaga
     6421 acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag atcaccaagg
     6481 cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac ctgaccctgc
     6541 tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc ttcgaccaga
     6601 gcaagaacgg ctacgccggc tacattgacg gcggagccag ccaggaagag ttctacaagt
     6661 tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg aagctgaaca
     6721 gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc caccagatcc
     6781 acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca ttcctgaagg
     6841 acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac gtgggccctc
     6901 tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc
     6961 cctggaactt cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc atcgagcgga
     7021 tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac agcctgctgt
     7081 acgagtactt caccgtgtat aacgagctga ccaaagtgaa atacgtgacc gagggaatga
     7141 gaaagcccgc tttcctgagc ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga
     7201 ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa atcgagtgtt
     7261 ttgatagtgt tgaaatttca ggagttgaag atagatttaa tgcttcatta ggtacctacc
     7321 atgatttgct aaaaattatt aaagataaag attttttgga taatgaagaa aatgaagata
     7381 tcttagagga tattgtttta acattgacct tatttgaaga tagggagatg attgaggaaa
     7441 gacttaaaac atatgctcac ctctttgatg ataaggtgat gaaacagctt aaacgtcgcc
     7501 gttatactgg ttggggacgt ttgtctcgaa aattgattaa tggtattagg gataagcaat
     7561 ctggcaaaac aatattagat tttttgaaat cagatggttt tgccaatcgc aattttatgc
     7621 agctgatcca tgatgatagt ttgacattta aagaagacat tcaaaaagca caagtgtctg
     7681 gacaaggcga tagtttacat gaacatattg caaatttagc tggtagccct gctattaaaa
     7741 aaggtatttt acagactgta aaagttgttg atgaattggt caaagtaatg gggcggcata
     7801 agccagaaaa tatcgttatt gaaatggcac gtgaaaatca gacaactcaa aagggccaga
     7861 aaaattcgcg agagcgtatg aaacgaatcg aagaaggtat caaagaatta ggaagtcaga
     7921 ttcttaaaga gcatcctgtt gaaaatactc aattgcaaaa tgaaaagctc tatctctatt
     7981 atctccaaaa tggaagagac atgtatgtgg accaagaatt agatattaat cgtttaagtg
     8041 attatgatgt cgatcacatt gttccacaaa gtttccttaa agacgattca atagacaata
     8101 aggtcttaac gcgttctgat aaaaatcgtg gtaaatcgga taacgttcca agtgaagaag
     8161 tagtcaaaaa gatgaaaaac tattggagac aacttctaaa cgccaagtta atcactcaac
     8221 gtaagtttga taatttaacg aaagctgaac gtggaggttt gagtgaactt gataaagctg
     8281 gttttatcaa acgccaattg gttgaaactc gccaaatcac taagcatgtg gcacaaattt
     8341 tggatagtcg catgaatact aaatacgatg aaaatgataa acttattcga gaggttaaag
     8401 tgattacctt aaaatctaaa ttagtttctg acttccgaaa agatttccaa ttctataaag
     8461 tacgtgagat taacaattac catcatgccc atgatgcgta tctaaatgcc gtcgttggaa
     8521 ctgctttgat taagaaatat ccaaaacttg aatcggagtt tgtctatggt gattataaag
     8581 tttatgatgt tcgtaaaatg attgctaagt ctgagcaaga aataggcaaa gcaaccgcaa
     8641 aatatttctt ttactctaat atcatgaact tcttcaaaac agaaattaca cttgcaaatg
     8701 gagagattcg caaacgccct ctaatcgaaa ctaatgggga aactggagaa attgtctggg
     8761 ataaagggcg agattttgcc acagtgcgca aagtattgtc catgccccaa gtcaatattg
     8821 tcaagaaaac agaagtacag acaggcggat tctccaagga gtcaatccgg cccaagcgga
     8881 acagcgataa gctcatcgcg cggaaaaaag attgggaccc taaaaagtat ggggggttct
     8941 tgtggccgac ggtcgcttac agcgtcttgg tcgtggcaaa ggtcgaaaag ggcaagtcga
     9001 aaaagctcaa atcggtcaaa gagttgttgg gtattacgat tatggagcgg tcctcgttcg
     9061 aaaagaaccc catcgacttt ctggaagcca aaggctataa ggaagtcaag aaggatctca
     9121 tcatcaaatt gccgaaatat tccctctttg aattggaaaa cggtcgtaag cgtatgctgg
     9181 cctcggcgaa gcaactgcag aaagggaacg aattggcgct cccttccaag tatgtgaact
     9241 tcctctacct cgcgtcgcac tacgagaagt tgaaagggtc gccagaagat aatgaacaaa
     9301 agcagctctt tgtggagcag cacaagcatt acctcgacga gattatcgag caaattagcg
     9361 agttcagcaa acgggtcatt ctcgccgacg ccaatctcga caaagtgctg tccgcataca
     9421 ataagcatcg ggacaaacca attcgggagc aagccgaaaa tatcatccac ctgtttacct
     9481 tgacccgcct gggtgcccct cgcgcgttca agtactttga taccaccatc gacccaaaac
     9541 agtaccggtc cacgaaggag gtcctcgatg ctaccctcat ccatcagagc attaccggcc
     9601 tctatgagac gcgcatcgac ctctcgcagc tggggggtga tagcggcggg agcggcggga
     9661 gcggggggag cactaacctc agcgacatta tcgagaagga aaccgggaag caactggtga
     9721 ttcaggagag catcctgatg ctccccgagg aggtggagga agtgatcggg aacaagccgg
     9781 agagcgacat cttggtccac accgcctatg acgaaagcac cgatgagaac gtcatgctct
     9841 tgacgtccga tgcgcctgag tacaagccgt gggccctcgt cattcaagac tccaatggtg
     9901 aaaacaaaat taagatgctg agcggaggat ccggaggatc tggaggcagc actaacctca
     9961 gcgacattat cgagaaggaa accgggaagc aactggtgat tcaggagagc atcctgatgc
    10021 tccccgagga ggtggaggaa gtgatcggga acaagccgga gagcgacatc ttggtccaca
    10081 ccgcctatga cgaaagcacc gatgagaacg tcatgctctt gacgtccgat gcgcctgagt
    10141 acaagccgtg ggccctcgtc attcaagact ccaatggtga aaacaaaatt aagatgtgat
    10201 ctagagtctt tatttgacat gcgtgatgtt tagaattata atttggggac cttaaggagg
    10261 ttttctaatg atcatgggaa ttcataaagg tgaagaactg ttcaccggtg ttgttccgat
    10321 cctggttgaa ctggatggtg atgttaacgg ccacaaattc tctgttcgtg gtgaaggtga
    10381 aggtgatgca accaacggta aactgaccct gaaattcatc tgcactaccg gtaaactgcc
    10441 ggttccatgg ccgactctgg tgactaccct gacctatggt gttcagtgtt tttctcgtta
    10501 cccggatcac atgaagcagc atgatttctt caaatctgca atgccggaag gttatgtaca
    10561 ggagcgcacc atttctttca aagacgatgg cacctacaaa acccgtgcag aggttaaatt
    10621 tgaaggtgat actctggtga accgtattga actgaaaggc attgatttca aagaggacgg
    10681 caacatcctg ggccacaaac tggaatataa cttcaactcc cataacgttt acatcaccgc
    10741 agacaaacag aagaacggta tcaaagctaa cttcaaaatt cgccataacg ttgaagacgg
    10801 tagcgtacag ctggcggacc actaccagca gaacactccg atcggtgatg gtccggttct
    10861 gctgccggat aaccactacc tgtccaccca gtctaaactg tccaaagacc cgaacgaaaa
    10921 gcgcgaccac atggtgctgc tggagttcgt tactgcagca ggtatcacgc acggcatgga
    10981 tgaactctac aaataaaagc ttgcggccgc gtcgtgactg ggaaaaccct ggcgactagt
    11041 cttggactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
    11101 acgctcggtt gccgccgggc gttttttatt ggtgagaatc caggggtccc tttaattaat
    11161 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
    11221 ctaaacccct aggccgcggc cgcgcggaga cccgagactg gtctcagttt tagagctaga
    11281 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt
    11341 gcaattcgag ctcggtaccc ggggatccca ataattacga tttacgtatt taaatgaacc
    11401 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
    11461 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
    11521 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
    11581 aagttaaaca tcatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta
    11641 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
    11701 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
    11761 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
    11821 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
    11881 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
    11941 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
    12001 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
    12061 gttcctgaac aggatc
//
