LOCUS       sequence-422549-        2776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone with ampicillin resistance for high expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdOm1.2
FEATURES             Location/Qualifiers
     source          1..2776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     525..545
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    749..973
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1043..1060)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1043..1059)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1059..1130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1131..1199,1200..1991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1349..1368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     1992..2011
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1992..2010
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2099..2117)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      2140..2728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2629..2648
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2747..2763
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2747..2763
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtgactagcg gaaacacaga aaaaagcccg cacctgacag tgcgggcttt ttttttcgac
       61 caaaggcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      541 gcgtgtacgg tgggaggtct atataagcag agctctccga agagccgagt gtcttcggtt
      601 cgagaccgaa ttcgcgagct ctcgctagcc agtttaaaca attgcggccg cgtactagtc
      661 tatcgatttg gatccaccct gcaggaagtc tagaccgtcg acacggtctc tgagagaaga
      721 cacgtcgctc ttcgacgtaa gtaactgact gtgccttcta gttgccagcc atctgttgtt
      781 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
      841 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
      901 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
      961 gtgggctcta tggctcggta ccaaattcca gaaaagaggc ctcccgaaag gggggccttt
     1021 tttcgttttg gtcctaagct taactggccg tcgttttaca ttcaaatatg tatccgctca
     1081 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     1141 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     1201 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     1261 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     1321 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     1381 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     1441 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     1501 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1561 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1621 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     1681 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1741 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     1801 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     1861 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1921 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1981 agcattggta ataatacgac tcactatagg gtaactaatt gaggcgcgcc taccaccgtc
     2041 aaaaaaaacg gcgcttttta gcgccgtttt tatttttcaa ccttcctagg taagtgaccc
     2101 tttagtgagg gttaatttag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     2161 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     2221 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     2281 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     2341 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     2401 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2461 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2521 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2581 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2641 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2701 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcccagg aaacagctat
     2761 gaccatgccc gggtaa
//
