LOCUS       sequence-422554-        2278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone with ampicillin resistance for intermediate 
            expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdOm2.3
FEATURES             Location/Qualifiers
     source          1..2278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        68..167
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     118..138
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    342..476
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(379..398)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     433..452
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(546..563)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(546..562)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        562..633
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(634..702,703..1494)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(852..871)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     1495..1514
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1495..1513
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1602..1620)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      1643..2231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2132..2151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2250..2266
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2250..2266
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agtgactagc ggaaacacag aaaaaagccc gcacctgaca gtgcgagctt tttttttcga
       61 ccaaaggcca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
      121 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc cgaagagccg
      181 agtgtcttcg gttcgagacc gaattcgcga gctctcgcta gccagtttaa acaattgcgg
      241 ccgcgtacta gtctatcgat ttggatccac cctgcaggaa gtctagaccg tcgacacggt
      301 ctctgagaga agacacgtcg ctcttcgacg taagtaactg aaacttgttt attgcagctt
      361 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
      421 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcctcg
      481 gtaccaaatt ccagaaaaga ggcctcccga aaggggggcc ttttttcgtt ttggtcctaa
      541 gcttaactgg ccgtcgtttt acattcaaat atgtatccgc tcatgagaca ataaccctga
      601 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      661 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      721 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
      781 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
      841 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
      901 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
      961 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1021 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1081 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1141 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     1201 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1261 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1321 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     1381 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     1441 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaataatac
     1501 gactcactat agggtaacta attgaggcgc gcctaccacc gtcaaaaaaa acggcgcttt
     1561 ttagcgccgt ttttattttt caaccttcct aggtaagtga ccctttagtg agggttaatt
     1621 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1681 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1741 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1801 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1861 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1921 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1981 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     2041 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2101 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2161 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2221 gcctatggaa aaacgccagc aacgcggccc aggaaacagc tatgaccatg cccgggta
//
