LOCUS       sequence-422557-       10455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Episomal plasmid encoding CasRx-P2A under the control of the CAG 
            promoter. The construct is flanked by PB and SB repeats for 
            integration into the cell genome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Montero JJ, Trozzo R, Sugden M, Ollinger R, Belka A, Zhigalova E, 
            Waetzig P, Engleitner T, Schmidt-Supprian M, Saur D, Rad R
  TITLE     Genome-scale pan-cancer interrogation of lncRNA dependencies using 
            CasRx.
  JOURNAL   Nat Methods. 2024 Feb 26. doi: 10.1038/s41592-024-02190-0.
  PUBMED    38409225
REFERENCE   2  (bases 1 to 10455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2024 Feb 26. doi: 10.1038/s41592-024-02190-0."
COMMENT     Sequence Label: pTransposon.CAG.CasRX-P2A-Blast
FEATURES             Location/Qualifiers
     source          1..10455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   704..766
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        1453..1832
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          2239..3254
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3178..3200
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3262..3281
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    3416..3440
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3487..3507
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3517..6414
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLRHWVVHNNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFRNKAVHLEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             6424..6444
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6448..6471
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6481..6537
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             6538..6936
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(6954..6978)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7019..7036)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7025..7249
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   8087..8121
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(8267..8285)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8306..8322)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8306..8322)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8319..8341)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8330..8346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8354..8360,8361..8378,8379..8384))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8399..8420
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8537..8554)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8708..9296)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8788..8807)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9467..10258,10259..10327))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10090..10109
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10328..10432)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg cggtattcac
      661 gacagcaggc tgaataataa aaaaattaga aactattatt taaccctaga aagataatca
      721 tattgtgacg tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct
      781 gtatatcgag gtttatttat taatttgaat agatattaag ttttattata tttacactta
      841 catactaata ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa
      901 caaaaactca aaatttcttc tataaagtaa caaaactttt aaacattctc tcttttacaa
      961 aaataaactt attttgtact ttaaaaacag tcatgttgta ttataaaata agtaattagc
     1021 ttaacctata cataatagaa acaaattata cttattagtc agtcagaaac aactttggca
     1081 catatcaata ttatgctctc gttaatcgcc gagctacagt tgaagtcgga agtttacata
     1141 cacttaagtt ggagtcatta aaactcgttt ttcaactact ccacaaattt cttgttaaca
     1201 aacaatagtt ttggcaagtc agttaggaca tctactttgt gcatgacaca agtcattttt
     1261 ccaacaattg tttacagaca gattatttca cttataattc actgtatcac aattccagtg
     1321 ggtcagaagt ttacatacac taagttgact gtgcctttaa acagcttgga aaattccaga
     1381 aaatgatgtc atggctttag aagcttactc tagcccgggc cccccctcga ggtcgatcag
     1441 ctttaagggc ccgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     1501 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     1561 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     1621 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     1681 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     1741 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     1801 acatctacgt attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca
     1861 ctctccccat ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat
     1921 tttgtgcagc gatgggggcg gggggggggg gggggggggg cccggggggg gggggggggg
     1981 gggggggggg gggggggggg gggggggggg gggggggggg ggccaaaaga gggggggcgc
     2041 gccggaaggt tctttttttg gggggggggg gggggggggg gccccctaaa aagggagccc
     2101 ggcggggggg gggggggggg gggggggggg gggagaggtg cggcggcagc caatcagggc
     2161 ggcgcgctcc gaaagtttcc ttttagggcg gggcggcggc ggcggcggcc ctataaaaag
     2221 cgaagcgcgc ggcgggcggg agtcgctgcg cgctgcctcc gccccgtgcc ccgctccgcc
     2281 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg
     2341 cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg gcttgtttct
     2401 tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg cggggggagc
     2461 ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg
     2521 cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc
     2581 gaggggagcg cggccggggg cggtgccccg cggtgcgggg gggctgcgag gggaacaaag
     2641 gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc
     2701 tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg
     2761 ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg
     2821 gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg
     2881 gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt
     2941 aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg
     3001 ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga
     3061 aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc
     3121 agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt
     3181 tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct
     3241 tctttttcct acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca
     3301 aagaattcta gaggcgcgcc ttaattaagg ccttaaaatt ctgcagtcga cgtcgacggt
     3361 atcgataagc ttgatatcga attcctgcag cccggggtac cgtcgacctc atcaaacaag
     3421 tttgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc ggtaccgacc
     3481 atgggcccca agaagaagag aaaggtggag gccagcatcg aaaaaaaaaa gtccttcgcc
     3541 aagggcatgg gcgtgaagtc cacactcgtg tccggctcca aagtgtacat gacaaccttc
     3601 gccgaaggca gcgacgccag gctggaaaag atcgtggagg gcgacagcat caggagcgtg
     3661 aatgagggcg aggccttcag cgctgaaatg gccgataaaa acgccggcta taagatcggc
     3721 aacgccaaat tcagccatcc taagggctac gccgtggtgg ctaacaaccc tctgtataca
     3781 ggacccgtcc agcaggatat gctcggcctg aaggaaactc tggaaaagag gtacttcggc
     3841 gagagcgctg atggcaatga caatatttgt atccaggtga tccataacat cctggacatt
     3901 gaaaaaatcc tcgccgaata cattaccaac gccgcctacg ccgtcaacaa tatctccggc
     3961 ctggataagg acattattgg attcggcaag ttctccacag tgtataccta cgacgaattc
     4021 aaagaccccg agcaccatag ggccgctttc aacaataacg ataagctcat caacgccatc
     4081 aaggcccagt atgacgagtt cgacaacttc ctcgataacc ccagactcgg ctatttcggc
     4141 caggcctttt tcagcaagga gggcagaaat tacatcatca attacggcaa cgaatgctat
     4201 gacattctgg ccctcctgag cggactgagg cactgggtgg tccataacaa cgaagaagag
     4261 tccaggatct ccaggacctg gctctacaac ctcgataaga acctcgacaa cgaatacatc
     4321 tccaccctca actacctcta cgacaggatc accaatgagc tgaccaactc cttctccaag
     4381 aactccgccg ccaacgtgaa ctatattgcc gaaactctgg gaatcaaccc tgccgaattc
     4441 gccgaacaat atttcagatt cagcattatg aaagagcaga aaaacctcgg attcaatatc
     4501 accaagctca gggaagtgat gctggacagg aaggatatgt ccgagatcag gaaaaatcat
     4561 aaggtgttcg actccatcag gaccaaggtc tacaccatga tggactttgt gatttatagg
     4621 tattacatcg aagaggatgc caaggtggct gccgccaata agtccctccc cgataatgag
     4681 aagtccctga gcgagaagga tatctttgtg attaacctga ggggctcctt caacgacgac
     4741 cagaaggatg ccctctacta cgatgaagct aatagaattt ggagaaagct cgaaaatatc
     4801 atgcacaaca tcaaggaatt taggggaaac aagacaagag agtataagaa gaaggacgcc
     4861 cctagactgc ccagaatcct gcccgctggc cgtgatgttt ccgccttcag caaactcatg
     4921 tatgccctga ccatgttcct ggatggcaag gagatcaacg acctcctgac caccctgatt
     4981 aataaattcg ataacatcca gagcttcctg aaggtgatgc ctctcatcgg agtcaacgct
     5041 aagttcgtgg aggaatacgc ctttttcaaa gactccgcca agatcgccga tgagctgagg
     5101 ctgatcaagt ccttcgctag aatgggagaa cctattgccg atgccaggag ggccatgtat
     5161 atcgacgcca tccgtatttt aggaaccaac ctgtcctatg atgagctcaa ggccctcgcc
     5221 gacacctttt ccctggacga gaacggaaac aagctcaaga aaggcaagca cggcatgaga
     5281 aatttcatta ttaataacgt gatcagcaat aaaaggttcc actacctgat cagatacggt
     5341 gatcctgccc acctccatga gatcgccaaa aacgaggccg tggtgaagtt cgtgctcggc
     5401 aggatcgctg acatccagaa aaaacagggc cagaacggca agaaccagat cgacaggtac
     5461 tacgaaactt gtatcggaaa ggataagggc aagagcgtga gcgaaaaggt ggacgctctc
     5521 acaaagatca tcaccggaat gaactacgac caattcgaca agaaaaggag cgtcattgag
     5581 gacaccggca gggaaaacgc cgagagggag aagtttaaaa agatcatcag cctgtacctc
     5641 accgtgatct accacatcct caagaatatt gtcaatatca acgccaggta cgtcatcgga
     5701 ttccattgcg tcgagcgtga tgctcaactg tacaaggaga aaggctacga catcaatctc
     5761 aagaaactgg aagagaaggg attcagctcc gtcaccaagc tctgcgctgg cattgatgaa
     5821 actgcccccg ataagagaaa ggacgtggaa aaggagatgg ctgaaagagc caaggagagc
     5881 attgacagcc tcgagagcgc caaccccaag ctgtatgcca attacatcaa atacagcgac
     5941 gagaagaaag ccgaggagtt caccaggcag attaacaggg agaaggccaa aaccgccctg
     6001 aacgcctacc tgaggaacac caagtggaat gtgatcatca gggaggacct cctgagaatt
     6061 gacaacaaga catgtaccct gttcagaaac aaggccgtcc acctggaagt ggccaggtat
     6121 gtccacgcct atatcaacga cattgccgag gtcaattcct acttccaact gtaccattac
     6181 atcatgcaga gaattatcat gaatgagagg tacgagaaaa gcagcggaaa ggtgtccgag
     6241 tacttcgacg ctgtgaatga cgagaagaag tacaacgata ggctcctgaa actgctgtgt
     6301 gtgcctttcg gctactgtat ccccaggttt aagaacctga gcatcgaggc cctgttcgat
     6361 aggaacgagg ccgccaagtt cgacaaggag aaaaagaagg tgtccggcaa ttccggatcc
     6421 ggacctaaga aaaagaggaa ggtggcagat tacaaagacg atgacgataa gggatccggc
     6481 gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg
     6541 gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac
     6601 agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc
     6661 atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg
     6721 ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat
     6781 gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat
     6841 cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt
     6901 gaattgctgc cctctggtta tgtgtgggag ggctaagcac tcgagatatc tagacccagc
     6961 tttcttgtac aaagtggttc gatgggcccc cgcgggcggc cgcgagctcg ctgatcagcc
     7021 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7081 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     7141 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     7201 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     7261 gaaagaacca gctggggctc gatcctctag ttgccaccgc ggtggctggg gctcgactag
     7321 agcttgcgga acccttcgtc tagtccatca caaagctctg acctcaatcc tatagaaagg
     7381 aggaatgagc caaaattcac ccaacttatt gtgggaagct tgtggaaggc tactcgaaat
     7441 gtttgaccca agttaaacaa tttaaaggca atgctaccaa atactaattg agtgtatgtt
     7501 aacttctgac ccactgggaa tgtgatgaaa gaaataaaag ctgaaatgaa tcattctctc
     7561 tactattatt ctgatatttc acattcttaa aataaagtgg tgatcctaac tgaccttaag
     7621 acagggaatc tttactcgga ttaaatgtca ggaattgtga aaaagtgagt ttaaatgtat
     7681 ttggctaagg tgtatgtaaa cttccgactt caactgtata tctagccaac aagctcgtca
     7741 tcgctttgca gaagagcaga gaggatatgc tcatcgtcta aagaactacc cattttatta
     7801 tatattagtc acgatatcta taacaagaaa atatatatat aataagttat cacgtaagta
     7861 gaacatgaaa taacaatata attatcgtat gagttaaatc ttaaaagtca cgtaaaagat
     7921 aatcatgcgt cattttgact cacgcggtcg ttatagttca aaatcagtga cacttaccgc
     7981 attgacaagc acgcctcacg ggagctccaa gcggcgactg agatgtccta aatgcacagc
     8041 gacggattcg cgctatttag aaagagagag caatatttca agaatgcatg cgtcaatttt
     8101 acgcagacta tctttctagg gttaaaaaag atttgcgctt tactcgacct aaactttaaa
     8161 cacgtcatag aatcttcgtt tgacaaaaac cacattgtgg ccaagctgtg tgacgcgacg
     8221 cgcgctaaag aatggcaaac caagtcgcgc gagccagctt ttgttccctt tagtgagggt
     8281 taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8341 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8401 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8461 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8521 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8581 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8641 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8701 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8761 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8821 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8881 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8941 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9001 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9061 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9121 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     9181 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9241 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9301 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9361 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9421 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9481 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9541 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9601 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9661 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9721 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9781 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9841 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     9901 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     9961 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10021 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10081 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10141 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10201 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10261 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10321 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10381 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10441 cccgaaaagt gccac
//
