LOCUS       sequence-422560-        2230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone with ampicillin resistance for low expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdOm2.4
FEATURES             Location/Qualifiers
     source          1..2230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(18..86,87..878)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(236..255)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     879..898
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        879..897
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(986..1004)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      1027..1615
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1516..1535
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1634..1650
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1634..1650
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1730..1781
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     1732..1752
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    1956..2090
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1993..2012)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2047..2066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2160..2177)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2160..2176)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(2176..2230,1..17)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
       61 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      121 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      181 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
      241 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
      301 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
      361 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
      421 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
      481 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
      541 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
      601 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
      661 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
      721 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
      781 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
      841 tcgctgagat aggtgcctca ctgattaagc attggtaata atacgactca ctatagggta
      901 actaattgag gcgcgcctac caccgtcaaa aaaaacggcg ctttttagcg ccgtttttat
      961 ttttcaacct tcctaggtaa gtgacccttt agtgagggtt aatttagaaa agatcaaagg
     1021 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     1081 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     1141 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     1201 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     1261 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     1321 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     1381 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     1441 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     1501 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     1561 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     1621 cagcaacgcg gcccaggaaa cagctatgac catgcccggg taagtgacta gcggaaacac
     1681 agaaaaaagc ccgcacctga cagtgcgggc tttttttttc gaccaaaggg acgcaaatgg
     1741 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctccgaaga gccgagtgtc
     1801 ttcggttcga gaccgaattc gcgagctctc gctagccagt ttaaacaatt gcggccgcgt
     1861 actagtctat cgatttggat ccaccctgca ggaagtctag accgtcgaca cggtctctga
     1921 gagaagacac gtcgctcttc gacgtaagta actgaaactt gtttattgca gcttataatg
     1981 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     2041 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc ctcggtacca
     2101 aattccagaa aagaggcctc ccgaaagggg ggcctttttt cgttttggtc ctaagcttaa
     2161 ctggccgtcg ttttacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2221 cttcaataat
//
