LOCUS       sequence-422561-        2223 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone with ampicillin resistance for low expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdOm2.5
FEATURES             Location/Qualifiers
     source          1..2223
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(20..88,89..880)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(238..257)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     881..900
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        881..899
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(988..1006)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      1029..1617
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1518..1537
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1636..1652
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1636..1652
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1732..1776
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     polyA_signal    1951..2085
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1988..2007)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2042..2061
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2155..2172)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2155..2171)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        join(2171..2223,1..19)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
       61 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      121 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      181 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      241 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      301 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      361 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
      421 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
      481 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
      541 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
      601 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
      661 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
      721 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
      781 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
      841 gatcgctgag ataggtgcct cactgattaa gcattggtaa taatacgact cactataggg
      901 taactaattg aggcgcgcct accaccgtca aaaaaaacgg cgctttttag cgccgttttt
      961 atttttcaac cttcctaggt aagtgaccct ttagtgaggg ttaatttaga aaagatcaaa
     1021 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1081 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1141 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1201 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1261 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1321 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1381 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1441 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1501 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1561 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1621 gccagcaacg cggcccagga aacagctatg accatgcccg ggtaagtgac tagcggaaac
     1681 acagaaaaaa gcccgcacct gacagtgcgg gctttttttt tcgaccaaag gatgggcggt
     1741 aggcgtgtac ggtgggaggt ctatataagc agagctctcc gaagagccga gtgtcttcgg
     1801 ttcgagaccg aattcgcgag ctctcgctag ccagtttaaa caattgcggc cgcgtactag
     1861 tctatcgatt tggatccacc ctgcaggaag tctagaccgt cgacacggtc tctgagagaa
     1921 gacacgtcgc tcttcgacgt aagtaactga aacttgttta ttgcagctta taatggttac
     1981 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2041 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcctcgg taccaaattc
     2101 cagaaaagag gcctcccgaa aggggggcct tttttcgttt tggtcctaag cttaactggc
     2161 cgtcgtttta cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     2221 ata
//
