LOCUS       sequence-422562-       10263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid used for the cloning of inmature gRNAs of the 
            Albarossa Library. The plasmid also encoded a puromycin resistance 
            cassette..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10263)
  AUTHORS   Montero JJ, Trozzo R, Sugden M, Ollinger R, Belka A, Zhigalova E, 
            Waetzig P, Engleitner T, Schmidt-Supprian M, Saur D, Rad R
  TITLE     Genome-scale pan-cancer interrogation of lncRNA dependencies using 
            CasRx.
  JOURNAL   Nat Methods. 2024 Feb 26. doi: 10.1038/s41592-024-02190-0.
  PUBMED    38409225
REFERENCE   2  (bases 1 to 10263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2024 Feb 26. doi: 10.1038/s41592-024-02190-0."
COMMENT     Sequence Label: plentiguide.U6.DR136(CasRX)_stuffer.puro
FEATURES             Location/Qualifiers
     source          1..10263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        82..1260
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          313..1251
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1208..1228
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1273..1872
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1273..1292)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1769..1789
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    1888..2476
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1941..1961)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2359..2370)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2548..2781
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2859..2980
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2896..2915)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2950..2969
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3020..3155
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3175..3194)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3176..3194)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3204..3221)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3204..3220)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3213..3235)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3362..3817
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3449..3468)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3659..3680
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3843..3947
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3948..4016,4017..4808)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4166..4185)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4979..5567
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5468..5487
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5721..5738
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5855..5876
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5891..5896,5897..5914,5915..5921)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5929..5945
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5934..5956
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5953..5969
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5953..5969
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5988..6008
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5990..6008
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6036..6262
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6263..6443
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6490..6615
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7108..7341
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7526..7570
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7748..7996
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7748..7768
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7919..7938
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7998..8033
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        9915..9950
                     /label=RfxCas13d DR36
                     /note="36-base direct repeat for an RfxCas13d pre-gRNA 
                     (Konermann et al., 2018)"
     misc_RNA        9958..10033
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    10089..10206
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gtgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
       61 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
      121 acagtccccg agaagttggg gggaggggtc ggcaattgat ccggtgccta gagaaggtgg
      181 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
      241 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
      301 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
      361 ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag
      421 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct
      481 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac
      541 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct
      601 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact
      661 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt
      721 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct
      781 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca
      841 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct
      901 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca
      961 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg
     1021 gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg
     1081 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg
     1141 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg
     1201 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
     1261 cgtacggcca ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     1321 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     1381 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     1441 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg
     1501 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     1561 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     1621 cggcccaagg agcccgcgtg gttcctggcc accgtcggag tctcgcccga ccaccagggc
     1681 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     1741 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     1801 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     1861 cccggtgcct gaacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     1921 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     1981 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2041 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2101 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2161 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2221 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2281 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     2341 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     2401 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     2461 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     2521 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     2581 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     2641 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     2701 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     2761 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     2821 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     2881 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     2941 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     3001 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     3061 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     3121 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     3181 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     3241 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     3301 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     3361 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     3421 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     3481 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     3541 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     3601 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     3661 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     3721 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     3781 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     3841 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3901 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3961 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4021 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4081 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4141 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4201 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4261 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4321 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4381 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     4441 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     4501 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4561 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4621 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     4681 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4741 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4801 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4861 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4921 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4981 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     5041 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     5101 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     5161 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     5221 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     5281 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     5341 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     5401 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     5461 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     5521 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     5581 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     5641 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     5701 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     5761 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     5821 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     5881 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     5941 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     6001 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     6061 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     6121 tgcatgccga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     6181 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     6241 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     6301 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6361 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6421 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     6481 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     6541 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     6601 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     6661 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     6721 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     6781 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     6841 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     6901 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     6961 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     7021 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     7081 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     7141 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     7201 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     7261 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     7321 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     7381 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     7441 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     7501 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     7561 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     7621 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     7681 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     7741 acccagagag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
     7801 tagagagata attagaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
     7861 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
     7921 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
     7981 gaaaggacga aacaccgcaa gtatacccct accaactggt cggggtttga aacggagacg
     8041 gttgtaaatg agcacacaaa atacacatgc taaaatatca tattctatga cctttataaa
     8101 atcaaccaaa atcttctttt taataacttt agtatcaata attagaattt ttatgttcct
     8161 ttttgcaaac ttttaataaa aatgagcaaa ataaaaaaac gctagtttta gtaactcgcg
     8221 ttgttttctt cacctttaat aatagctact ccaccacttg ttcctaagcg gtcagctcct
     8281 gcttcaatca ttttttgagc atcttcaaat gttctaactc caccagctgc tttaactaaa
     8341 gcattgtctt taacaactga cttcattagt ttaacatctt caaatgttgc acctgatttt
     8401 gaaaatcctg ttgatgtttt aacaaattct aatccagctt caacagctat ttcacaagct
     8461 ttcatgattt cttcttttgt taataaacaa ttttccataa tacatttaac aacatgtgat
     8521 ccagctgctt tttttacagc tttcatgtct tctaaaacta attcataatt tttgtctttt
     8581 aatgcaccaa tatttaatac catatcaatt tctgttgcac catctttaat tgcttcagaa
     8641 acttcgaatg cttttgtagc tgttgtgcat gcacctagag gaaaacctac aacatttgtt
     8701 attcctacat ttgtgccttt taataattct ttacaatagc ttgttcaata tgaattaaca
     8761 caaactgttg caaaatcaaa ttcaattgct tcatcacata attgtttaat ttcagctttc
     8821 gtagcatctt gttttaataa tgtgtgatct atatatttgt ttagtttcat tttttctcct
     8881 atatattcat ttttaatttt aattctttaa taatttcgtc tactttaact ttagcgtttt
     8941 gaacagattc accaacacct ataaaataaa tttttagttt aggttcagtt ccacttgggc
     9001 gaacagcaaa tcatgactta tcttctaaat aaaattttag taagtcttgt cctggcatat
     9061 tatacattcc atcgatgtag tcttcaacat taacaacttt aagtccagca atttgagtta
     9121 agggtgttgc tctcaatgat ttcattaatg gttcaatttt taatttcttt tcttctggtt
     9181 taaaattcaa gtttaaagtg aaagtgtaat atgcacccat ttctttaaat aaatcttcta
     9241 aatagtctac taatgtttta ttttgttttt tataaaatca agcagcctct gctattaata
     9301 tagaagcttg tattccatct ttatctctag ctgagtcatc aattacatat ccataacttt
     9361 cttcataagc aaaaacaaaa tttaatccgt tatcttcttc tttagcaatt tctctaccca
     9421 ttcatttaaa tccagttaaa gtttttacaa tattaactcc atatttttca tgagcgattc
     9481 tatcacccaa atcacttgtt acaaaacttg aatatagagc cggatttttt ggaatgctat
     9541 ttaagcgttt tagatttgat aattttcaat caattaaaat tggtcctgtt tgatttccat
     9601 ctaatcttac aaaatgacca tcatgtttta ttgccattcc aaatctgtca gcatctgggt
     9661 cattcataat aataatatct gcatcatgtt taataccata ttcaagcggt atttttcatg
     9721 caggatcaaa ttctggattt ggatttacaa catttttaaa tgtttcatct tcaaatgcat
     9781 gctcttcaac ctcaataacg ttatatcctg attcacgtaa tatttttggg gtaaatttag
     9841 ttcctgttcc attaactgcg ctaaaaataa tttttaaatc ttttttagct tcttgctctt
     9901 ttttgtacgt ctctcaagta tacccctacc aactggtcgg ggtttgaaac tttttttgtt
     9961 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
    10021 accgagtcgg tgctttttta agcttggcgt aactagatct tgagacaaat ggcagtattc
    10081 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
    10141 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
    10201 aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct cgagggggcc
    10261 cgg
//
