LOCUS       sequence-422563-        2973 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone with ampicillin resistance for high expression in 
            mammalian cells with an added 5'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdOm1.0
FEATURES             Location/Qualifiers
     source          1..2973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      23..611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     512..531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     630..646
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..646
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        726..1105
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1106..1309
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1260..1280
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        1354..1372
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1354..1371
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    1605..1829
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1899..1916)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1899..1915)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1915..1986
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1987..2055,2056..2847)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2205..2224)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     2848..2867
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2848..2866
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2955..2973)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggccc aggaaacagc tatgaccatg cccgggtaag
      661 tgactagcgg aaacacagaa aaaagcccgc acctgacagt gcgggctttt tttttcgacc
      721 aaagggacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      781 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      841 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      901 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      961 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1021 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1081 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1141 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     1201 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     1261 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac
     1321 tagagaaccc actgcttact ggcttatcga aatatttagg tgacactata gagacccaag
     1381 cttggcattc cggtactgtt ggtaaaactc tcgaaacttg gctagctccc ctccgaagag
     1441 ccgagtgtct tcggttcgag accgaattcg cgagctctcg ctagccagtt taaacaattg
     1501 cggccgcgta ctagtctatc gatttggatc caccctgcag gaagtctaga ccgtcgacac
     1561 ggtctctgag agaagacacg tcgctcttcg acgtaagtaa ctgactgtgc cttctagttg
     1621 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     1681 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     1741 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     1801 gcatgctggg gatgcggtgg gctctatggc tcggtaccaa attccagaaa agaggcctcc
     1861 cgaaaggggg gccttttttc gttttggtcc taagcttaac tggccgtcgt tttacattca
     1921 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     1981 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     2041 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     2101 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     2161 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     2221 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     2281 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     2341 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     2401 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     2461 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     2521 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     2581 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     2641 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     2701 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     2761 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     2821 ggtgcctcac tgattaagca ttggtaataa tacgactcac tatagggtaa ctaattgagg
     2881 cgcgcctacc accgtcaaaa aaaacggcgc tttttagcgc cgtttttatt tttcaacctt
     2941 cctaggtaag tgacccttta gtgagggtta att
//
