LOCUS       sequence-422564-        2320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone with ampicillin resistance for low expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdOm1.4
FEATURES             Location/Qualifiers
     source          1..2320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        67..118
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     69..89
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    293..517
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(587..604)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(587..603)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        603..674
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(675..743,744..1535)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(893..912)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     1536..1555
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1536..1554
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1643..1661)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      1684..2272
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2173..2192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2291..2307
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2291..2307
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtgactagcg gaaacacaga aaaaagcccg cacctgacag tgcgggcttt ttttttcgac
       61 caaagggacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
      121 ccgaagagcc gagtgtcttc ggttcgagac cgaattcgcg agctctcgct agccagttta
      181 aacaattgcg gccgcgtact agtctatcga tttggatcca ccctgcagga agtctagacc
      241 gtcgacacgg tctctgagag aagacacgtc gctcttcgac gtaagtaact gactgtgcct
      301 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
      361 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
      421 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
      481 aatagcaggc atgctgggga tgcggtgggc tctatggctc ggtaccaaat tccagaaaag
      541 aggcctcccg aaaggggggc cttttttcgt tttggtccta agcttaactg gccgtcgttt
      601 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      661 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      721 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      781 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      841 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
      901 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
      961 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     1021 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     1081 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     1141 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     1201 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     1261 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     1321 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     1381 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1441 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1501 ctgagatagg tgcctcactg attaagcatt ggtaataata cgactcacta tagggtaact
     1561 aattgaggcg cgcctaccac cgtcaaaaaa aacggcgctt tttagcgccg tttttatttt
     1621 tcaaccttcc taggtaagtg accctttagt gagggttaat ttagaaaaga tcaaaggatc
     1681 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1741 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1801 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1861 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1921 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1981 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     2041 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     2101 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     2161 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2221 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2281 caacgcggcc caggaaacag ctatgaccat gcccgggtaa
//
