LOCUS       sequence-422568-        2762 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone with ampicillin resistance for high expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdOm2.1
FEATURES             Location/Qualifiers
     source          1..2762
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        68..447
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        448..651
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     602..622
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    826..960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(863..882)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     917..936
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1030..1047)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1030..1046)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1046..1117
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1118..1186,1187..1978)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1336..1355)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     primer_bind     1979..1998
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1979..1997
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2086..2104)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      2127..2715
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2616..2635
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2734..2750
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2734..2750
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agtgactagc ggaaacacag aaaaaagccc gcacctgaca gtgcgggctt tttttttcga
       61 ccaaagggac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      121 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      181 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      241 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      301 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      361 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      421 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      481 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      541 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      601 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctccgaaga
      661 gccgagtgtc ttcggttcga gaccgaattc gcgagctctc gctagccagt ttaaacaatt
      721 gcggccgcgt actagtctat cgatttggat ccaccctgca ggaagtctag accgtcgaca
      781 cggtctctga gagaagacac gtcgctcttc gacgtaagta actgaaactt gtttattgca
      841 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
      901 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
      961 ctcggtacca aattccagaa aagaggcctc ccgaaagggg ggcctttttt cgttttggtc
     1021 ctaagcttaa ctggccgtcg ttttacattc aaatatgtat ccgctcatga gacaataacc
     1081 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     1141 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     1201 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     1261 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     1321 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     1381 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     1441 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     1501 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     1561 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     1621 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     1681 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     1741 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     1801 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     1861 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     1921 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaata
     1981 atacgactca ctatagggta actaattgag gcgcgcctac caccgtcaaa aaaaacggcg
     2041 ctttttagcg ccgtttttat ttttcaacct tcctaggtaa gtgacccttt agtgagggtt
     2101 aatttagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2161 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2221 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2281 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2341 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2401 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2461 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2521 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2581 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2641 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2701 cggagcctat ggaaaaacgc cagcaacgcg gcccaggaaa cagctatgac catgcccggg
     2761 ta
//
