LOCUS       sequence-422569-        2226 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone with trimethoprim resistance for high expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdOm5.1
FEATURES             Location/Qualifiers
     source          1..2226
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        70..449
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        450..653
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     604..624
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    828..1052
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(1122..1139)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1122..1138)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1142..1189
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1208..1444
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     primer_bind     1445..1464
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1445..1463
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1552..1570)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      1593..2181
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2082..2101
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2200..2216
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2200..2216
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 taagtgacta gcggaaacac agaaaaaagc ccgcacctga cagtgcgggc tttttttttc
       61 gaccaaaggg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      121 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      181 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      241 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      301 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      361 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      421 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      481 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      541 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      601 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctccgaa
      661 gagccgagtg tcttcggttc gagaccgaat tcgcgagctc tcgctagcca gtttaaacaa
      721 ttgcggccgc gtactagtct atcgatttgg atccaccctg caggaagtct agaccgtcga
      781 cacggtctct gagagaagac acgtcgctct tcgacgtaag taactgactg tgccttctag
      841 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
      901 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
      961 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     1021 caggcatgct ggggatgcgg tgggctctat ggctcggtac caaattccag aaaagaggcc
     1081 tcccgaaagg ggggcctttt ttcgttttgg tcctaagctt aactggccgt cgttttacac
     1141 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     1201 ctaaaccatg ggtcaaagta gcgatgaagc caacgctccc gttgcagggc agtttgcgct
     1261 tcccctgagt gccacctttg gcttagggga tcgcgtacgc aagaaatctg gtgccgcttg
     1321 gcagggtcaa gtcgtcggtt ggtattgcac aaaactcact cctgaaggct atgcggtcga
     1381 gtccgaatcc cacccaggct cagtgcaaat ttatcctgtg gctgcacttg aacgtgtggc
     1441 ctagtaatac gactcactat agggtaacta attgaggcgc gcctaccacc gtcaaaaaaa
     1501 acggcgcttt ttagcgccgt ttttattttt caaccttcct aggtaagtga ccctttagtg
     1561 agggttaatt tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1621 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1681 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1741 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1801 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1861 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1921 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     1981 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2041 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2101 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2161 ggggggcgga gcctatggaa aaacgccagc aacgcggccc aggaaacagc tatgaccatg
     2221 cccggg
//
