LOCUS       sequence-422570-        2136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty backbone with trimethoprim resistance for high expression in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Manigat FO, Connell LB, Stewart BN, LePabic AR, Tessier CJG, Emlaw 
            JR, Calvert ND, Rossl A, Shuhendler AJ, daCosta CJB, Campbell-Valois
            FX
  TITLE     pUdOs: Concise Plasmids for Bacterial and Mammalian Cells.
  JOURNAL   ACS Synth Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408.
  PUBMED    38235654
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Jan 18. doi: 10.1021/acssynbio.3c00408."
COMMENT     Sequence Label: pUdOm6.1
FEATURES             Location/Qualifiers
     source          1..2136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        71..450
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        451..654
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     605..625
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    829..963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(866..885)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     920..939
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1033..1050)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1033..1049)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1053..1100
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1119..1355
                     /codon_start=1
                     /gene="dhfrIIb from plasmid R388"
                     /product="E. coli plasmid-associated dihydrofolate 
                     reductase"
                     /label=TpR
                     /note="confers resistance to trimethoprim"
                     /translation="MGQSSDEANAPVAGQFALPLSATFGLGDRVRKKSGAAWQGQVVGW
                     YCTKLTPEGYAVESESHPGSVQIYPVAALERVA"
     primer_bind     1356..1375
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1356..1374
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1463..1481)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      1504..2092
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1993..2012
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2111..2127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2111..2127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gtaagtgact agcggaaaca cagaaaaaag cccgcacctg acagtgcggg cttttttttt
       61 cgaccaaagg gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
      121 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      181 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      241 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      301 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      361 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      421 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      481 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      541 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      601 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccga
      661 agagccgagt gtcttcggtt cgagaccgaa ttcgcgagct ctcgctagcc agtttaaaca
      721 attgcggccg cgtactagtc tatcgatttg gatccaccct gcaggaagtc tagaccgtcg
      781 acacggtctc tgagagaaga cacgtcgctc ttcgacgtaa gtaactgaaa cttgtttatt
      841 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
      901 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
      961 atcctcggta ccaaattcca gaaaagaggc ctcccgaaag gggggccttt tttcgttttg
     1021 gtcctaagct taactggccg tcgttttaca ctgttgacaa ttaatcatcg gcatagtata
     1081 tcggcatagt ataatacgac aaggtgagga actaaaccat gggtcaaagt agcgatgaag
     1141 ccaacgctcc cgttgcaggg cagtttgcgc ttcccctgag tgccaccttt ggcttagggg
     1201 atcgcgtacg caagaaatct ggtgccgctt ggcagggtca agtcgtcggt tggtattgca
     1261 caaaactcac tcctgaaggc tatgcggtcg agtccgaatc ccacccaggc tcagtgcaaa
     1321 tttatcctgt ggctgcactt gaacgtgtgg cctagtaata cgactcacta tagggtaact
     1381 aattgaggcg cgcctaccac cgtcaaaaaa aacggcgctt tttagcgccg tttttatttt
     1441 tcaaccttcc taggtaagtg accctttagt gagggttaat ttagaaaaga tcaaaggatc
     1501 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1561 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1621 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1681 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1741 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1801 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1861 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1921 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1981 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     2041 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     2101 caacgcggcc caggaaacag ctatgaccat gcccgg
//
