LOCUS       sequence-42259-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of human L-MYC and LIN28.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Okita K, Matsumura Y, Sato Y, Okada A, Morizane A, Okamoto S, Hong 
            H, Nakagawa M, Tanabe K, Tezuka KI, Shibata T, Kunisada T, Takahashi
            M, Takahashi J, Saji H, Yamanaka S
  TITLE     A more efficient method to generate integration-free human iPS 
            cells.
  JOURNAL   Nat Methods. 2011 May;8(5):409-12
  PUBMED    21460823
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-05"; volume: "8(5)"; pages: "409-1"
COMMENT     Sequence Label: pCXLE-hUL
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(735..800)
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
ORIGIN
        1 tctttaaatt ttgtaatcca gaggttgatt atcatatgac tagtccccga agcttgaatt
       61 cctgcaggca attcgccctt tcaattctgt gcctccggga gcagggtagg gctgtggatt
      121 tcttcttctt cctctcgaaa gtaggttggc tttccctgtg cactagggcc ctgctgggcc
      181 ttcagcggac atgaggctac catatggctg atgctctggc agaagtggca cttcttgggc
      241 tggggtggca gcttgcattc cttggcatga tgatctagac ctccacagtt gtagcacctg
      301 tctccttttg atctgcgctt ctgcatgctc tttccttttg gccgcctctc actcccaata
      361 cagaatactc caccaggtcc ggtgacacgg atggattcca gacccttggc tgacttctta
      421 aaggtgaact ccactgcctc accctccttc aagctccgga acccttccat gtgcagctta
      481 ctctggtgca caaagacatc cactgggggg tcgagcgcga ccccggcgcg ggcggtcatg
      541 gacaggaagc cgaaccccat gcgcacgttg aaccacttac agatgcccgc accgtgcagc
      601 agctgaggct cgtccgccgc ccgggccgcg tcctccggcg cctcctcggg cgcctcttct
      661 gccgccttgg cgcagccacc tgcaaactgc tggttggaca cggagcccat ggtgaagggc
      721 gaattgttca tgagtggacc tggattgctt tctacatccc cagccagttt gagtaaatca
      781 aagttaagag tttgtttgac aggagcgaca attttgagat ctccgtagcc agtgaggta
//
