LOCUS       sequence-422616-        3802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Arabinose-inducible himar1C9 transposase expression - cam 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   English MA, Alcantar MA, Collins JJ
  TITLE     A self-propagating, barcoded transposon system for the dynamic 
            rewiring of genomic networks.
  JOURNAL   Mol Syst Biol. 2023 Jun 12;19(6):e11398. doi: 
            10.15252/msb.202211398. Epub 2023 Mar 27.
  PUBMED    36970845
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2023-06-12"; volume: "19(6):e11398. doi"; pages: " 
            10.15252/msb.202211398. Epub 2023 Mar 27"
COMMENT     Sequence Label: pHelper_AraC/pBad-Tnpase (camR)
FEATURES             Location/Qualifiers
     source          1..3802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(1..546)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(81..100)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             662..1321
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(728..747)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1392..1426
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1431..2309)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2336..2620
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2543..2562
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             2638..2649
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg
       61 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg
      121 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc
      181 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc
      241 acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
      301 acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag
      361 ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc
      421 gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa
      481 ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga
      541 tctcaagaag atcatcttga tcgggcacgt aagaggttcc aactttcacc ataatgaaat
      601 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
      661 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
      721 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
      781 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
      841 tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa tgaaagacgg
      901 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
      961 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     1021 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     1081 gaatatgttt ttcgtttcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     1141 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     1201 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     1261 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     1321 atttgatatc gagctcgctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
     1381 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt ttatgacaac
     1441 ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct cgggctggcc
     1501 ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga
     1561 ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt
     1621 tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag atgtgacaga
     1681 cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc
     1741 aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg tggatggagc
     1801 gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt tatcgccagc
     1861 agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa caggtcgctg
     1921 aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa tattgacggc
     1981 cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg gtgataccat
     2041 tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa cagcaaaata
     2101 tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg accgcgaatg
     2161 gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa atcgagataa
     2221 ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga gtatcccggc
     2281 agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt cagagaagaa
     2341 accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg gctcttctcg
     2401 ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg gaccaaagcc
     2461 atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca cattgattat
     2521 ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt agcggatcct
     2581 acctgacgct ttttatcgca actctctact gtttctccat aagaattcaa aagatctaaa
     2641 gaggagaaag gatctatgga aaaaaaggaa tttcgtgttt tgataaaata ctgttttctg
     2701 aagggaaaaa atacagtgga agcaaaaact tggcttgata atgagtttcc ggactctgcc
     2761 ccggggaaat caacaataat tgattggtat gcaaaattca agcgtggtga aatgagcacg
     2821 gaggacggtg aacgcagtgg acgcccgaaa gaggtggtta ccgacgaaaa catcaaaaaa
     2881 atccacaaaa tgattttgaa tgaccgtaaa atgaagttga tcgagatagc agaggcctta
     2941 aagatatcaa aggaacgtgt tggtcatatc attcatcaat atttggatat gcggaagctc
     3001 tgtgcgaaat gggtgccgcg cgagctcaca tttgaccaaa aacaacgacg tgttgatgat
     3061 tctaagcggt gtttgcagct gttaactcgt aatacacccg agtttttccg tcgatatgtg
     3121 acaatggatg aaacatggct ccatcactac actcctgagt ccaatcgaca gtcggctgag
     3181 tggacagcga ccggtgaacc gtctccgaag cgtggaaaga ctcaaaagtc cgctggcaaa
     3241 gtaatggcct ctgttttttg ggatgcgcat ggaataattt ttatcgatta tcttgagaag
     3301 ggaaaaacca tcaacagtga ctattatatg gcgttattgg agcgtttgaa ggtcgaaatc
     3361 gcggcaaaac ggccccacat gaagaagaaa aaagtgttgt tccaccaaga caacgcaccg
     3421 tgccacaagt cattgagaac gatggcaaaa attcatgaat tgggcttcga attgcttccc
     3481 cacccgccgt attctccaga tctggccccc agcgactttt tcttgttctc agacctcaaa
     3541 aggatgctcg cagggaaaaa atttggctgc aatgaagagg tgatcgccga aactgaggcc
     3601 tattttgagg caaaaccgaa ggagtactac caaaatggta tcaaaaaatt ggaaggtcgt
     3661 tataatcgtt gtatcgctct tgaagggaac tatgttgaat aactgtaaca gagcattagc
     3721 gcaaggtgat ttttgtcttc ttgcgctaat tttttcagga agatacttaa tagggaagtg
     3781 agagggccgc ggcaaagccg tt
//
