LOCUS       sequence-422623-        4047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mariner transposon expressing mCherry with cam selection casette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   English MA, Alcantar MA, Collins JJ
  TITLE     A self-propagating, barcoded transposon system for the dynamic 
            rewiring of genomic networks.
  JOURNAL   Mol Syst Biol. 2023 Jun 12;19(6):e11398. doi: 
            10.15252/msb.202211398. Epub 2023 Mar 27.
  PUBMED    36970845
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2023-06-12"; volume: "19(6):e11398. doi"; pages: " 
            10.15252/msb.202211398. Epub 2023 Mar 27"
COMMENT     Sequence Label: pDonor_Tn[RFP]
FEATURES             Location/Qualifiers
     source          1..4047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(18..809,810..878))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     641..660
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(879..983)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1051..1069
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             2291..2950
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2357..2376)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      3021..3055
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     rep_origin      3289..3677
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     oriT            3792..3901
                     /note="incP origin of transfer"
ORIGIN
        1 ccctcgaatt gacgcgttta ccaatgctta atcagtgagg cacctatctc agcgatctgt
       61 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
      121 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
      181 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
      241 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
      301 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
      361 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
      421 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
      481 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
      541 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
      601 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
      661 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
      721 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
      781 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
      841 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
      901 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
      961 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     1021 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtcac
     1081 gcgtcctcgg tacctaacag gttggatgat aagtccccgg tcttcgtatg ccgtcttctg
     1141 cttggcgcgc cctcgacgtc tccagcttcg tacgcggccg ttgacaatta atcatccggc
     1201 tcgtaactta tgtggactcg agagcgctca acgggtgtgc ttcccgttct gatgagtccg
     1261 tgaggacgaa agcgcctcta caaataattt tgtttaaaaa tttaatgatg agtacaaaga
     1321 agtactaagg aaggaaaata cccaatggtt tctaagggcg aagaagataa tatggccatt
     1381 atcaaggagt ttatgcgctt taaagtgcac atggagggct cagtaaacgg tcacgaattt
     1441 gaaatcgaag gagaaggcga gggccgtccg tacgagggga ctcagacggc gaagctcaaa
     1501 gtcacaaaag gtggcccatt gccattcgcc tgggatattc tgtcgccgca gttcatgtat
     1561 ggcagcaaag cgtacgtaaa acacccggca gatattccgg attatctgaa attatctttc
     1621 ccggaaggtt tcaaatggga acgcgttatg aatttcgagg acgggggcgt agtgacggtc
     1681 acgcaggata gtagcctgca ggacggcgaa ttcatttaca aagtcaagct gcgtggcaca
     1741 aatcttccgt ctgatggccc ggtgatgcag aagaaaacca tgggttggga ggcttccagc
     1801 gaacgcatgt atcccgaaga tggagcattg aaaggcgaaa tcaaacagcg cctgaaatta
     1861 aaagacggcg gacattacga cgccgaggtc aagaccactt acaaagcgaa aaaaccagtt
     1921 cagctgccag gcgcctacaa cgttaatatc aagctggata ttacctcgca taatgaagat
     1981 tatactatcg tggaacaata cgagcgcgcc gaaggccgcc actccactgg tggtatggat
     2041 gaattatata aataaggatc ctaaacattt aataaaaaaa gggcggtcgc aagatcgccc
     2101 ttttttacgt atgacacctc gtctcaacca aagcaatcaa ccagtgaaaa atggcgccca
     2161 tcggcgccat ttttttatgt ctagattgat cgggcacgta agaggttcca actttcacca
     2221 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
     2281 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
     2341 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
     2401 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
     2461 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat
     2521 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
     2581 gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct
     2641 acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg
     2701 gtttattgag aatatgtttt tcgtttcagc caatccctgg gtgagtttca ccagttttga
     2761 tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta
     2821 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     2881 tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg
     2941 cggggcgtaa tttgatatcg agctcgcttg gactcctgtt gatagatcca gtaatgacct
     3001 cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtc
     3061 tggtctcaca attgtccggc cattaaaaaa gcggctaacc acgccgcttt ttttacgtct
     3121 gcactcggta ccaaattcca gaaaagaggc ctcccgaaag gggggccttt tttcgttttg
     3181 gtcccccggg aacctgcagg gcgcgccaag cagaagacgg catacgaaga ccggggactt
     3241 atcatccaac ctgttaggat cagtgagcgc aacggaatct aattcccatg tcagccgtta
     3301 agtgttcctg tgtcactcaa aattgctttg agaggctcta agggcttctc agtgcgttac
     3361 atccctggct tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc
     3421 ttttttttct tataaaactt aaaaccttag aggctattta agttgctgat ttatattaat
     3481 tttattgttc aaacatgaga gcttagtacg tgaaacatga gagcttagta cgttagccat
     3541 gagagcttag tacgttagcc atgagggttt agttcgttaa acatgagagc ttagtacgtt
     3601 aaacatgaga gcttagtacg tgaaacatga gagcttagta cgtactatca acaggttgaa
     3661 ctgctgatct tcagatcctc tacgccggac gcatcgtggc cggatcaatt ccgtgatagg
     3721 tgggctgccc ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac
     3781 gccggcggta gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga
     3841 ataagggaca gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc
     3901 cggctgacgc cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt
     3961 atatcgtgcg aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg
     4021 ttatgcagcg gaaaacggaa ttgatcc
//
