LOCUS       sequence-422626-       11403 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic integration of genes (inserted in I-SceI-site) via 
            transposon Tn5 with GmR and PE-H.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11403)
  AUTHORS   Weihmann R, Kubicki S, Bitzenhofer NL, Domrose A, Bator I, Kirschen 
            LM, Kofler F, Funk A, Tiso T, Blank LM, Jaeger KE, Drepper T, Thies 
            S, Loeschcke A
  TITLE     The modular pYT vector series employed for chromosomal gene 
            integration and expression to produce carbazoles and glycolipids in 
            P. putida.
  JOURNAL   FEMS Microbes. 2022 Dec 19;4:xtac030. doi: 10.1093/femsmc/xtac030. 
            eCollection 2023.
  PUBMED    37333445
REFERENCE   2  (bases 1 to 11403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbes."; date: "2022-12-19"; volume: "4:xtac030. doi"; pages: " 
            10.1093/femsmc/xtac030. eCollection 2023"
COMMENT     Sequence Label: pYTRW17K_6G5
FEATURES             Location/Qualifiers
     source          1..11403
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(2778..2783,2784..2800,2801..2806)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2995..3528
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     misc_feature    5703..5995
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      6643..7096
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(7124..7146)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7232..7372
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7287..7306
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7387..7404)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7558..8146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7638..7657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8325..9119)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8436..8455)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     9046..9065
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        9558..9773
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             9774..10577
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgaac tttagaagga
     1681 gatataccat gccatttaac aagaaaagcg ttcttgccct ctgtggcgct ggcgcgctgc
     1741 tgttctcgat gtctgccctg gccaacaacc cggcaccgac cgatccgggt gattcgggcg
     1801 gtggttccgc ctatcagcgc ggtccggatc cgtccgtcag cttcctcgaa gccgatcgcg
     1861 gtcagtacag cgtccgttcc agccgtgtct ccagcctggt cagcggcttt ggcggcggca
     1921 ccatttacta tccgacaggc acaaccggca ctatgggtgc ggtcgttgta attcccggct
     1981 tcgtttctgc cgagtcttcg attgactggt ggggcccgaa actggcgtcc tatggtttcg
     2041 tggtcatgac cattgacacc aacaccggct ttgatcagcc gccgagccgt gcgcgtcaga
     2101 tcaacaacgc actggattat ctggttagcc agaacagccg cagctccagc ccggttcgcg
     2161 gcatgatcga caccaatcgt ctgggcgtca ttggctggtc gatgggcggt ggcggcaccc
     2221 tgcgtgttgc cagtgaaggt cgtatcaagg cagccattcc gctggcgccc tgggatacaa
     2281 ccagctacta cgccagccgc tcgcaggcac cgactctgat ctttgcctgt gagtcggatg
     2341 tgatcgcgcc ggtcctccag catgcctcac cgttctacaa ttccctgcct tccagcatcg
     2401 acaaggcctt tgtggaaatc aatggtggca gccactactg cggtaatggc ggcagcatct
     2461 acaacgatgt gctgagccgg ttcggggtgt cctggatgaa actgcatctg gatgaagaca
     2521 gccgctacaa gcagttcctc tgcggaccga accacacttc cgactctcag atctccgatt
     2581 atcgcggcaa ctgcccgtac taataacgcg cgcgtaacta taacggtcct aaggtagcga
     2641 gcttacttgt gctgttgtgt aaggacacgc ttaacccaga gaattccagc gacctattct
     2701 acgactacgc gtccatggct cgaggagctc ggtaccgccc cactacccga tttatcaaga
     2761 tttggcggag agtcccattg acataagcct gttcggttcg taaactgtaa tgcaagtagc
     2821 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     2881 tggcggtttt catggcttgt tatgactgtt tttttgtaca gtctatgcct cgggcatcca
     2941 agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta
     3001 cgcagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aggtggctca
     3061 agtatgggca tcattcgcac atgtaggctc ggccctgacc aagtcaaatc catgcgggct
     3121 gctcttgatc ttttcggtcg tgagttcgga gacgtagcca cctactccca acatcagccg
     3181 gactccgatt acctcgggaa cttgctccgt agtaagacat tcatcgcgct tgctgccttc
     3241 gaccaagaag cggttgttgg cgctctcgcg gcttacgttc tgcccaagtt tgagcagccg
     3301 cgtagtgaga tctatatcta tgatctcgca gtctccggcg agcaccggag gcagggcatt
     3361 gccaccgcgc tcatcaatct cctcaagcat gaggccaacg cgcttggtgc ttatgtgatc
     3421 tacgtgcaag cagattacgg tgacgatccc gcagtggctc tctatacaaa gttgggcata
     3481 cgggaagaag tgatgcactt tgatatcgac ccaagtaccg ccacctaagt cgactggcaa
     3541 acagctatta tgggtattat gggtgtgtat ccagaacgac ggatcttgac gcaatttggc
     3601 gtggctctct taaggtagcc ctccgaccaa ttgaggtgcg ctctgaccga tcagtttaga
     3661 cttgtgtata agagtcagag cgtcctgaac ggaacctttc ccgttttcca ggatctgatc
     3721 ttccatgtga cctcctaaca tggtaacgtt catgataact tctgctcttc atcgtgcggc
     3781 cgactgggct aaatctgtgt tctcttcggc ggcgctgggt gatcctcgcc gtactgcccg
     3841 cttggttaac gtcgccgccc aattggcaaa atattctggt aaatcaataa ccatctcatc
     3901 agagggtagt gaagccatgc aggaaggcgc ttaccgattt atccgcaatc ccaacgtttc
     3961 tgccgaggcg atcagaaagg ctggcgccat gcaaacagtc aagttggctc aggagtttcc
     4021 cgaactgctg gccattgagg acaccacctc tttgagttat cgccaccagg tcgccgaaga
     4081 gcttggcaag ctgggctcta ttcaggataa atcccgcgga tggtgggttc actccgttct
     4141 cttgctcgag gccaccacat tccgcaccgt aggattactg catcaggagt ggtggatgcg
     4201 cccggatgac cctgccgatg cggatgaaaa ggagagtggc aaatggctgg cagcggccgc
     4261 aactagccgg ttacgcatgg gcagcatgat gagcaacgtg attgcggtct gtgaccgcga
     4321 agccgatatt catgcttatc tgcaggacaa actggcgcat aacgagcgct tcgtggtgcg
     4381 ctccaagcac ccacgcaagg acgtagagtc tgggttgtat ctgtacgacc atctgaagaa
     4441 ccaaccggag ttgggtggct atcagatcag cattccgcaa aagggcgtgg tggataaacg
     4501 cggtaaacgt aaaaatcgac cagcccgcaa ggcgagcttg agcctgcgca gtgggcgcat
     4561 cacgctaaaa caggggaata tcacgctcaa cgcggtgctg gccgaggaga ttaacccgcc
     4621 caagggtgag accccgttga aatggttgtt gctgaccagc gaaccggtcg agtcgctagc
     4681 ccaagccttg cgcgtcatcg acatttatac ccatcgctgg cggatcgagg agttccataa
     4741 ggcatggaaa accggagcag gagccgagag gcaacgcatg gaggagccgg ataatctgga
     4801 gcggatggtc tcgatcctct cgtttgttgc ggtcaggctg ttacagctca gagaaagctt
     4861 cacgctgccg caagcactca gggcgcaagg gctgctaaag gaagcggaac acgtagaaag
     4921 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa
     4981 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc
     5041 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg
     5101 ggaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat
     5161 ggcgcagggg atcaagatct gacccgggac tagttggtgt ggaagtccta atatcggtga
     5221 tcaatatagt ggttgacatg ctggctagtc aacattgagc cttttgatca tgcaaatata
     5281 ttacggtatt ttacaatcaa atatcaaact taactattga ctttataact tatttaggtg
     5341 gtaacattct tataaaaaag aaaaaaatta ctgcaaaaca gtactagctt ttaacttgta
     5401 tcctaggtta tctatgctgt ctcaccatag agaatattac ctatttcaga atgtatgtcc
     5461 atgattcgcc gggtaaatac atataataca caaatctggc ttaataaagt ctataatata
     5521 tctcataaag aagtgctaaa ttggctagtg ctatatattt ttaagaaaat ttcttttgac
     5581 taagtccata tcgactttgt aaaagttcac attagcatac atatattaca cgagccagaa
     5641 atagtaactt ttgcctaaat cacaaattgc aaaatttaat tgcttgcaaa aggtcacatg
     5701 cttataatca acttttttaa aaatttaaaa tactttttta ttttttattt ttaaacataa
     5761 atgaaataat ttatttattg tttatgatta ccgaaacata aaacctgctc aagaaaaaga
     5821 aactgttttg tccttggaaa aaaagcacta cctaggagcg gccaaaatgc cgaggctttc
     5881 atagcttaaa ctctttacag aaaataggca ttatagatca gttcgagttt tcttattctt
     5941 ccttccggtt ttatcgtcac agttttacag taaataagta tcacctctta gagttaacct
     6001 atactaaaat ttatgccagt taactatgag ataagcaagt atcatctcat ttcattacct
     6061 gaagtcgagt aaacagaaaa tccaattgtt gatgaacctc aatgacttag aactatctat
     6121 cggcagatca tataaagagg atttaggtac tcgagaacgc tccgtacatg cagctaaacc
     6181 tccaagcatc tctaacttgg gtagtgcttt ctccaccatt ttctgtgctt gctccttcgt
     6241 ggcaagtcca gcccataatg cccagaatgt agttgcggat tcgtatgacg ttctgtgctt
     6301 gatttttgtg ttgtagtcaa agaaaaggtc cggttaaacg gatctcgcat tgatgaggca
     6361 acgctaatta tcaacatata gattgttatc tatctgcatg aacacgaaat ctttacttga
     6421 cgacttgagg ctgatggtgt ttatgcaaag aaaccactgt gtttaatatg tgtcactgtt
     6481 tgatattact gtcagcgtag aagataatag taaaagcggt taataagtgt atttgagata
     6541 agtgtgataa agtttttaca gcgaaaagac gataaataca agaaaatgat tacgaggata
     6601 cggagagagg tatgtacatg tgtatttata tactaagctg ccggcggttg tttgcaagac
     6661 cgagaaaagg ctagcaagaa tcgggtcatt gtagcgtatg cgcctgtgaa cattctcttc
     6721 aacaagtttg attccattgc ggtgaaatgg taaaagtcaa ccccctgcga tgtatatttt
     6781 cctgtacaat caatcaaaaa gccaaatgat ttagcattat ctttacatct tgttatttta
     6841 cagattttat gtttagatct tttatgcttg cttttcaaaa ggcctgcagg caagtgcaca
     6901 aacaatactt aaataaatac tactcagtaa taacctattt cttagcattt ttgacgaaat
     6961 ttgctatttt gttagagtct tttacaccat ttgtctccac acctccgctt acatcaacac
     7021 caataacgcc atttaatcta agcgcatcac caacattttc tggcgtcagt ccaccagcta
     7081 acataaaatg ttgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     7141 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     7201 gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata
     7261 gcggagtgta tactggctta actatgcggc atcagagcag attgtactga gagtgcacca
     7321 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc
     7381 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7441 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7501 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7561 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7621 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7681 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7741 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7801 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7861 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7921 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7981 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     8041 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     8101 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     8161 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     8221 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     8281 aatctaaagt atatatgagt aaacttggtc tgacaggagc tcgctcagaa gaactcgtca
     8341 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
     8401 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
     8461 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
     8521 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg
     8581 tcgggcatcc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
     8641 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
     8701 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
     8761 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
     8821 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
     8881 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgg
     8941 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
     9001 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
     9061 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
     9121 cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca tcagatcctt
     9181 ggcggcaaga aagccatcca gtttactttg cagggcttcc caaccttacc agagggcgcc
     9241 ccagctggca attccggttc gcttgctgtc cataaaaccg cccagtctag ctatcgccat
     9301 gtaagcccac tgcaagctac ctgctttctc tttgcgcttg cgttttccct tgtccagata
     9361 gcccagtagc tgacattcat ccgggcgatc gatggaagcc ggcggcacct cgctaacgga
     9421 ttcaccactc caagaattgg agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc
     9481 aacccttggc agaacatatc catcgcgtcc gccatctcca gcagccgcac gcggcgcatc
     9541 gggggggggg gggggntttc aattcatcat ttttttttta ttcttttttt tgatttcggt
     9601 ttccttgaaa tttttttgat tcggtaatct ccgaacagaa ggaagaacga aggaaggagc
     9661 acagacttag attggtatat atacgcatat gtagtgttga agaaacatga aattgcccag
     9721 tattcttaac ccaactgcac agaacaaaaa cctgcaggaa acgaagataa atcatgtcga
     9781 aagctacata taaggaacgt gctgctactc atcctagtcc tgttgctgcc aagctattta
     9841 atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt accaccaagg
     9901 aattactgga gttagttgaa gcattaggtc ccaaaatttg tttactaaaa acacatgtgg
     9961 atatcttgac tgatttttcc atggagggca cagttaagcc gctaaaggca ttatccgcca
    10021 agtacaattt tttactcttc gaagacagaa aatttgctga cattggtaat acagtcaaat
    10081 tgcagtactc tgcgggtgta tacagaatag cagaatgggc agacattacg aatgcacacg
    10141 gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg
    10201 aacctagagg ccttttgatg ttagcagaat tgtcatgcaa gggctcccta tctactggag
    10261 aatatactaa gggtactgtt gacattgcga agagcgacaa agattttgtt atcggcttta
    10321 ttgctcaaag agacatgggt ggaagagatg aaggttacga ttggttgatt atgacacccg
    10381 gtgtgggttt agatgacaag ggagacgcat tgggtcaaca gtatagaacc gtggatgatg
    10441 tggtctctac aggatctgac attattattg ttggaagagg actatttgca aagggaaggg
    10501 atgctaaggt agagggtgaa cgttacagaa aagcaggctg ggaagcatat ttgagaagat
    10561 gcggccagca aaactaaaaa actgtattat aagtaaatgc atgtatacta aactcacaaa
    10621 ttagagcttc aatttaatta tatcagttat tacccgggaa tctcggtcgt aatgattttt
    10681 ataatgacga aaaaaaaaaa attggaaaga aaaccccccc ccccscmgcg ttgggtcctg
    10741 gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg
    10801 ccttactggt tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg
    10861 caaaacgtct gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag
    10921 tctggaaacg cggaagtcag cgccctgcac cattatgttc cggatctgca tcgcaggatg
    10981 ctgctggcta ccctgtggaa cacctacatc tgtattaacg aagcgctggc attgaccctg
    11041 agtgattttt ctctggtccc gccgcatcca taccgccagt tgtttaccct cacaacgttc
    11101 cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg agcatcctct ctcgtttcat
    11161 cggtatcatt acccccatga acagaaatcc cccttacacg gaggcatcag tgaccaaaca
    11221 ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga
    11281 gaaactcaac gagctggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca
    11341 cgctgatgag ctttaccgct gggccattct catgaagaat atcttgaatt tattgtcata
    11401 tta
//
