LOCUS       sequence-422651-       10977 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic integration of genes (inserted in I-SceI-site) via 
            transposon Tn5 with TcR .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10977)
  AUTHORS   Weihmann R, Kubicki S, Bitzenhofer NL, Domrose A, Bator I, Kirschen 
            LM, Kofler F, Funk A, Tiso T, Blank LM, Jaeger KE, Drepper T, Thies 
            S, Loeschcke A
  TITLE     The modular pYT vector series employed for chromosomal gene 
            integration and expression to produce carbazoles and glycolipids in 
            P. putida.
  JOURNAL   FEMS Microbes. 2022 Dec 19;4:xtac030. doi: 10.1093/femsmc/xtac030. 
            eCollection 2023.
  PUBMED    37333445
REFERENCE   2  (bases 1 to 10977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbes."; date: "2022-12-19"; volume: "4:xtac030. doi"; pages: " 
            10.1093/femsmc/xtac030. eCollection 2023"
COMMENT     Sequence Label: pYTRW09K_0T5
FEATURES             Location/Qualifiers
     source          1..10977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(1836..1841,1842..1858,1859..1864)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1912..3102
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(2264..2283)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    5277..5569
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      6217..6670
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(6698..6720)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6806..6946
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6861..6880
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6961..6978)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7132..7720)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7212..7231)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7899..8693)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8010..8029)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8620..8639
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        9132..9347
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             9348..10151
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgtaa ctataacggt
     1681 cctaaggtag cgagcttact tgtgctgttg tgtaaggaca cgcttaaccc agagaattcc
     1741 agcgacctat tctacgacta cgcgtccatg gctcgaggag ctcggtaccg ccccactacc
     1801 cgatttatca agatttggcg gagagtccct catgtttgac agcttatcat cgataagctt
     1861 taatgcggta gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct
     1921 aacaatgcgc tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg
     1981 gttatgccgg tactgccggg cctcttgcgg gatatcgtcc attccgacag catcgccagt
     2041 cactatggcg tgctgctagc gctatatgcg ttgatgcaat ttctatgcgc acccgttctc
     2101 ggagcactgt ccgaccgctt tggccgccgc ccagtcctgc tcgcttcgct acttggagcc
     2161 actatcgact acgcgatcat ggcgaccaca cccgtcctgt ggatcctcta cgccggacgc
     2221 atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat cgccgacatc
     2281 accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt cggcgtgggt
     2341 atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca tgcaccattc
     2401 cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct aatgcaggag
     2461 tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc
     2521 cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa
     2581 ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg
     2641 agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa
     2701 gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc
     2761 atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc
     2821 ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg
     2881 ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct cgcggctctt
     2941 accagcctaa cttcgatcat tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg
     3001 agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt ctgcctcccc
     3061 gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gagtcgactg gcaaacagct
     3121 attatgggta ttatgggtgt gtatccagaa cgacggatct tgacgcaatt tggcgtggct
     3181 ctcttaaggt agccctccga ccaattgagg tgcgctctga ccgatcagtt tagacttgtg
     3241 tataagagtc agagcgtcct gaacggaacc tttcccgttt tccaggatct gatcttccat
     3301 gtgacctcct aacatggtaa cgttcatgat aacttctgct cttcatcgtg cggccgactg
     3361 ggctaaatct gtgttctctt cggcggcgct gggtgatcct cgccgtactg cccgcttggt
     3421 taacgtcgcc gcccaattgg caaaatattc tggtaaatca ataaccatct catcagaggg
     3481 tagtgaagcc atgcaggaag gcgcttaccg atttatccgc aatcccaacg tttctgccga
     3541 ggcgatcaga aaggctggcg ccatgcaaac agtcaagttg gctcaggagt ttcccgaact
     3601 gctggccatt gaggacacca cctctttgag ttatcgccac caggtcgccg aagagcttgg
     3661 caagctgggc tctattcagg ataaatcccg cggatggtgg gttcactccg ttctcttgct
     3721 cgaggccacc acattccgca ccgtaggatt actgcatcag gagtggtgga tgcgcccgga
     3781 tgaccctgcc gatgcggatg aaaaggagag tggcaaatgg ctggcagcgg ccgcaactag
     3841 ccggttacgc atgggcagca tgatgagcaa cgtgattgcg gtctgtgacc gcgaagccga
     3901 tattcatgct tatctgcagg acaaactggc gcataacgag cgcttcgtgg tgcgctccaa
     3961 gcacccacgc aaggacgtag agtctgggtt gtatctgtac gaccatctga agaaccaacc
     4021 ggagttgggt ggctatcaga tcagcattcc gcaaaagggc gtggtggata aacgcggtaa
     4081 acgtaaaaat cgaccagccc gcaaggcgag cttgagcctg cgcagtgggc gcatcacgct
     4141 aaaacagggg aatatcacgc tcaacgcggt gctggccgag gagattaacc cgcccaaggg
     4201 tgagaccccg ttgaaatggt tgttgctgac cagcgaaccg gtcgagtcgc tagcccaagc
     4261 cttgcgcgtc atcgacattt atacccatcg ctggcggatc gaggagttcc ataaggcatg
     4321 gaaaaccgga gcaggagccg agaggcaacg catggaggag ccggataatc tggagcggat
     4381 ggtctcgatc ctctcgtttg ttgcggtcag gctgttacag ctcagagaaa gcttcacgct
     4441 gccgcaagca ctcagggcgc aagggctgct aaaggaagcg gaacacgtag aaagccagtc
     4501 cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa
     4561 acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact
     4621 gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc tctgggaagg
     4681 ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca
     4741 ggggatcaag atctgacccg ggactagttg gtgtggaagt cctaatatcg gtgatcaata
     4801 tagtggttga catgctggct agtcaacatt gagccttttg atcatgcaaa tatattacgg
     4861 tattttacaa tcaaatatca aacttaacta ttgactttat aacttattta ggtggtaaca
     4921 ttcttataaa aaagaaaaaa attactgcaa aacagtacta gcttttaact tgtatcctag
     4981 gttatctatg ctgtctcacc atagagaata ttacctattt cagaatgtat gtccatgatt
     5041 cgccgggtaa atacatataa tacacaaatc tggcttaata aagtctataa tatatctcat
     5101 aaagaagtgc taaattggct agtgctatat atttttaaga aaatttcttt tgactaagtc
     5161 catatcgact ttgtaaaagt tcacattagc atacatatat tacacgagcc agaaatagta
     5221 acttttgcct aaatcacaaa ttgcaaaatt taattgcttg caaaaggtca catgcttata
     5281 atcaactttt ttaaaaattt aaaatacttt tttatttttt atttttaaac ataaatgaaa
     5341 taatttattt attgtttatg attaccgaaa cataaaacct gctcaagaaa aagaaactgt
     5401 tttgtccttg gaaaaaaagc actacctagg agcggccaaa atgccgaggc tttcatagct
     5461 taaactcttt acagaaaata ggcattatag atcagttcga gttttcttat tcttccttcc
     5521 ggttttatcg tcacagtttt acagtaaata agtatcacct cttagagtta acctatacta
     5581 aaatttatgc cagttaacta tgagataagc aagtatcatc tcatttcatt acctgaagtc
     5641 gagtaaacag aaaatccaat tgttgatgaa cctcaatgac ttagaactat ctatcggcag
     5701 atcatataaa gaggatttag gtactcgaga acgctccgta catgcagcta aacctccaag
     5761 catctctaac ttgggtagtg ctttctccac cattttctgt gcttgctcct tcgtggcaag
     5821 tccagcccat aatgcccaga atgtagttgc ggattcgtat gacgttctgt gcttgatttt
     5881 tgtgttgtag tcaaagaaaa ggtccggtta aacggatctc gcattgatga ggcaacgcta
     5941 attatcaaca tatagattgt tatctatctg catgaacacg aaatctttac ttgacgactt
     6001 gaggctgatg gtgtttatgc aaagaaacca ctgtgtttaa tatgtgtcac tgtttgatat
     6061 tactgtcagc gtagaagata atagtaaaag cggttaataa gtgtatttga gataagtgtg
     6121 ataaagtttt tacagcgaaa agacgataaa tacaagaaaa tgattacgag gatacggaga
     6181 gaggtatgta catgtgtatt tatatactaa gctgccggcg gttgtttgca agaccgagaa
     6241 aaggctagca agaatcgggt cattgtagcg tatgcgcctg tgaacattct cttcaacaag
     6301 tttgattcca ttgcggtgaa atggtaaaag tcaaccccct gcgatgtata ttttcctgta
     6361 caatcaatca aaaagccaaa tgatttagca ttatctttac atcttgttat tttacagatt
     6421 ttatgtttag atcttttatg cttgcttttc aaaaggcctg caggcaagtg cacaaacaat
     6481 acttaaataa atactactca gtaataacct atttcttagc atttttgacg aaatttgcta
     6541 ttttgttaga gtcttttaca ccatttgtct ccacacctcc gcttacatca acaccaataa
     6601 cgccatttaa tctaagcgca tcaccaacat tttctggcgt cagtccacca gctaacataa
     6661 aatgttgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     6721 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     6781 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     6841 tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
     6901 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc
     6961 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     7021 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     7081 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     7141 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     7201 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     7261 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     7321 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     7381 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     7441 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     7501 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     7561 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     7621 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     7681 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     7741 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     7801 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     7861 aagtatatat gagtaaactt ggtctgacag gagctcgctc agaagaactc gtcaagaagg
     7921 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     7981 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     8041 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     8101 accatgatat tcggcaagca ggcatcgcca tgggtcacga cgagatcctc gccgtcgggc
     8161 atccgcgcct tgagcctggc gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc
     8221 agatcatcct gatcgacaag accggcttcc atccgagtac gtgctcgctc gatgcgatgt
     8281 ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca
     8341 tcagccatga tggatacttt ctcggcagga gcaaggtgag atgacaggag atcctgcccc
     8401 ggcacttcgc ccaatagcag ccagtccctt cccgcttcag tgacaacgtc gagcacagct
     8461 gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg ctgcctcgtc ttggagttca
     8521 ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc
     8581 cggaacacgg cggcatcaga gcagccgatt gtctgttgtg cccagtcata gccgaatagc
     8641 ctctccaccc aagcggccgg agaacctgcg tgcaatccat cttgttcaat catgcgaaac
     8701 gatcctcatc ctgtctcttg atcagatctt gatcccctgc gccatcagat ccttggcggc
     8761 aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg cgccccagct
     8821 ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg ccatgtaagc
     8881 ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca gatagcccag
     8941 tagctgacat tcatccgggc gatcgatgga agccggcggc acctcgctaa cggattcacc
     9001 actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct
     9061 tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catcgggggg
     9121 gggggggggn tttcaattca tcattttttt tttattcttt tttttgattt cggtttcctt
     9181 gaaatttttt tgattcggta atctccgaac agaaggaaga acgaaggaag gagcacagac
     9241 ttagattggt atatatacgc atatgtagtg ttgaagaaac atgaaattgc ccagtattct
     9301 taacccaact gcacagaaca aaaacctgca ggaaacgaag ataaatcatg tcgaaagcta
     9361 catataagga acgtgctgct actcatccta gtcctgttgc tgccaagcta tttaatatca
     9421 tgcacgaaaa gcaaacaaac ttgtgtgctt cattggatgt tcgtaccacc aaggaattac
     9481 tggagttagt tgaagcatta ggtcccaaaa tttgtttact aaaaacacat gtggatatct
     9541 tgactgattt ttccatggag ggcacagtta agccgctaaa ggcattatcc gccaagtaca
     9601 attttttact cttcgaagac agaaaatttg ctgacattgg taatacagtc aaattgcagt
     9661 actctgcggg tgtatacaga atagcagaat gggcagacat tacgaatgca cacggtgtgg
     9721 tgggcccagg tattgttagc ggtttgaagc aggcggcaga agaagtaaca aaggaaccta
     9781 gaggcctttt gatgttagca gaattgtcat gcaagggctc cctatctact ggagaatata
     9841 ctaagggtac tgttgacatt gcgaagagcg acaaagattt tgttatcggc tttattgctc
     9901 aaagagacat gggtggaaga gatgaaggtt acgattggtt gattatgaca cccggtgtgg
     9961 gtttagatga caagggagac gcattgggtc aacagtatag aaccgtggat gatgtggtct
    10021 ctacaggatc tgacattatt attgttggaa gaggactatt tgcaaaggga agggatgcta
    10081 aggtagaggg tgaacgttac agaaaagcag gctgggaagc atatttgaga agatgcggcc
    10141 agcaaaacta aaaaactgta ttataagtaa atgcatgtat actaaactca caaattagag
    10201 cttcaattta attatatcag ttattacccg ggaatctcgg tcgtaatgat ttttataatg
    10261 acgaaaaaaa aaaaattgga aagaaaaccc ccccccccgc agcgttgggt cctggccacg
    10321 ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac
    10381 tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac
    10441 gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga
    10501 aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg
    10561 gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat
    10621 ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa
    10681 ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat
    10741 cattaccccc atgaacagaa atccccctta cacggaggca tcagtgacca aacaggaaaa
    10801 aaccgccctt aacatggccc gctttatcag aagccagaca ttaacgcttc tggagaaact
    10861 caacgagctg gacgcggatg aacaggcaga catctgtgaa tcgcttcacg accacgctga
    10921 tgagctttac cgctgggcca ttctcatgaa gaatatcttg aatttattgt catatta
//
