LOCUS       sequence-422656-       11204 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic integration of genes (inserted in I-SceI-site) via 
            transposon Tn5 with GmR and eYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11204)
  AUTHORS   Weihmann R, Kubicki S, Bitzenhofer NL, Domrose A, Bator I, Kirschen 
            LM, Kofler F, Funk A, Tiso T, Blank LM, Jaeger KE, Drepper T, Thies 
            S, Loeschcke A
  TITLE     The modular pYT vector series employed for chromosomal gene 
            integration and expression to produce carbazoles and glycolipids in 
            P. putida.
  JOURNAL   FEMS Microbes. 2022 Dec 19;4:xtac030. doi: 10.1093/femsmc/xtac030. 
            eCollection 2023.
  PUBMED    37333445
REFERENCE   2  (bases 1 to 11204)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbes."; date: "2022-12-19"; volume: "4:xtac030. doi"; pages: " 
            10.1093/femsmc/xtac030. eCollection 2023"
COMMENT     Sequence Label: pYTRW16K_1G5
FEATURES             Location/Qualifiers
     source          1..11204
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1689..1691,1692..1694,1695..2408)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1734..1755)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1995..2014)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2342..2363
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        join(2580..2585,2586..2602,2603..2608)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2797..3330
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     misc_feature    5505..5797
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      6445..6898
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(6926..6948)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7034..7174
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7089..7108
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7189..7206)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7360..7948)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7440..7459)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8127..8921)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8238..8257)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8848..8867
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        9359..9574
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             9575..10378
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgaac tttagaagga
     1681 gatataccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1741 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1801 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1861 ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc taccccgacc
     1921 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1981 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     2041 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     2101 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     2161 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2221 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2281 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2341 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2401 acaagtaacg cgcgcgtaac tataacggtc ctaaggtagc gagcttactt gtgctgttgt
     2461 gtaaggacac gcttaaccca gagaattcca gcgacctatt ctacgactac gcgtccatgg
     2521 ctcgaggagc tcggtaccgc cccactaccc gatttatcaa gatttggcgg agagtcccat
     2581 tgacataagc ctgttcggtt cgtaaactgt aatgcaagta gcgtatgcgc tcacgcaact
     2641 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     2701 gttatgactg tttttttgta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg
     2761 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag caacgatgtt
     2821 acgcagcagg gcagtcgccc taaaacaaag ttaggtggct caagtatggg catcattcgc
     2881 acatgtaggc tcggccctga ccaagtcaaa tccatgcggg ctgctcttga tcttttcggt
     2941 cgtgagttcg gagacgtagc cacctactcc caacatcagc cggactccga ttacctcggg
     3001 aacttgctcc gtagtaagac attcatcgcg cttgctgcct tcgaccaaga agcggttgtt
     3061 ggcgctctcg cggcttacgt tctgcccaag tttgagcagc cgcgtagtga gatctatatc
     3121 tatgatctcg cagtctccgg cgagcaccgg aggcagggca ttgccaccgc gctcatcaat
     3181 ctcctcaagc atgaggccaa cgcgcttggt gcttatgtga tctacgtgca agcagattac
     3241 ggtgacgatc ccgcagtggc tctctataca aagttgggca tacgggaaga agtgatgcac
     3301 tttgatatcg acccaagtac cgccacctaa gtcgactggc aaacagctat tatgggtatt
     3361 atgggtgtgt atccagaacg acggatcttg acgcaatttg gcgtggctct cttaaggtag
     3421 ccctccgacc aattgaggtg cgctctgacc gatcagttta gacttgtgta taagagtcag
     3481 agcgtcctga acggaacctt tcccgttttc caggatctga tcttccatgt gacctcctaa
     3541 catggtaacg ttcatgataa cttctgctct tcatcgtgcg gccgactggg ctaaatctgt
     3601 gttctcttcg gcggcgctgg gtgatcctcg ccgtactgcc cgcttggtta acgtcgccgc
     3661 ccaattggca aaatattctg gtaaatcaat aaccatctca tcagagggta gtgaagccat
     3721 gcaggaaggc gcttaccgat ttatccgcaa tcccaacgtt tctgccgagg cgatcagaaa
     3781 ggctggcgcc atgcaaacag tcaagttggc tcaggagttt cccgaactgc tggccattga
     3841 ggacaccacc tctttgagtt atcgccacca ggtcgccgaa gagcttggca agctgggctc
     3901 tattcaggat aaatcccgcg gatggtgggt tcactccgtt ctcttgctcg aggccaccac
     3961 attccgcacc gtaggattac tgcatcagga gtggtggatg cgcccggatg accctgccga
     4021 tgcggatgaa aaggagagtg gcaaatggct ggcagcggcc gcaactagcc ggttacgcat
     4081 gggcagcatg atgagcaacg tgattgcggt ctgtgaccgc gaagccgata ttcatgctta
     4141 tctgcaggac aaactggcgc ataacgagcg cttcgtggtg cgctccaagc acccacgcaa
     4201 ggacgtagag tctgggttgt atctgtacga ccatctgaag aaccaaccgg agttgggtgg
     4261 ctatcagatc agcattccgc aaaagggcgt ggtggataaa cgcggtaaac gtaaaaatcg
     4321 accagcccgc aaggcgagct tgagcctgcg cagtgggcgc atcacgctaa aacaggggaa
     4381 tatcacgctc aacgcggtgc tggccgagga gattaacccg cccaagggtg agaccccgtt
     4441 gaaatggttg ttgctgacca gcgaaccggt cgagtcgcta gcccaagcct tgcgcgtcat
     4501 cgacatttat acccatcgct ggcggatcga ggagttccat aaggcatgga aaaccggagc
     4561 aggagccgag aggcaacgca tggaggagcc ggataatctg gagcggatgg tctcgatcct
     4621 ctcgtttgtt gcggtcaggc tgttacagct cagagaaagc ttcacgctgc cgcaagcact
     4681 cagggcgcaa gggctgctaa aggaagcgga acacgtagaa agccagtccg cagaaacggt
     4741 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa
     4801 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat
     4861 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tgggaaggtt gggaagccct
     4921 gcaaagtaaa ctggatggct ttcttgccgc caaggatctg atggcgcagg ggatcaagat
     4981 ctgacccggg actagttggt gtggaagtcc taatatcggt gatcaatata gtggttgaca
     5041 tgctggctag tcaacattga gccttttgat catgcaaata tattacggta ttttacaatc
     5101 aaatatcaaa cttaactatt gactttataa cttatttagg tggtaacatt cttataaaaa
     5161 agaaaaaaat tactgcaaaa cagtactagc ttttaacttg tatcctaggt tatctatgct
     5221 gtctcaccat agagaatatt acctatttca gaatgtatgt ccatgattcg ccgggtaaat
     5281 acatataata cacaaatctg gcttaataaa gtctataata tatctcataa agaagtgcta
     5341 aattggctag tgctatatat ttttaagaaa atttcttttg actaagtcca tatcgacttt
     5401 gtaaaagttc acattagcat acatatatta cacgagccag aaatagtaac ttttgcctaa
     5461 atcacaaatt gcaaaattta attgcttgca aaaggtcaca tgcttataat caactttttt
     5521 aaaaatttaa aatacttttt tattttttat ttttaaacat aaatgaaata atttatttat
     5581 tgtttatgat taccgaaaca taaaacctgc tcaagaaaaa gaaactgttt tgtccttgga
     5641 aaaaaagcac tacctaggag cggccaaaat gccgaggctt tcatagctta aactctttac
     5701 agaaaatagg cattatagat cagttcgagt tttcttattc ttccttccgg ttttatcgtc
     5761 acagttttac agtaaataag tatcacctct tagagttaac ctatactaaa atttatgcca
     5821 gttaactatg agataagcaa gtatcatctc atttcattac ctgaagtcga gtaaacagaa
     5881 aatccaattg ttgatgaacc tcaatgactt agaactatct atcggcagat catataaaga
     5941 ggatttaggt actcgagaac gctccgtaca tgcagctaaa cctccaagca tctctaactt
     6001 gggtagtgct ttctccacca ttttctgtgc ttgctccttc gtggcaagtc cagcccataa
     6061 tgcccagaat gtagttgcgg attcgtatga cgttctgtgc ttgatttttg tgttgtagtc
     6121 aaagaaaagg tccggttaaa cggatctcgc attgatgagg caacgctaat tatcaacata
     6181 tagattgtta tctatctgca tgaacacgaa atctttactt gacgacttga ggctgatggt
     6241 gtttatgcaa agaaaccact gtgtttaata tgtgtcactg tttgatatta ctgtcagcgt
     6301 agaagataat agtaaaagcg gttaataagt gtatttgaga taagtgtgat aaagttttta
     6361 cagcgaaaag acgataaata caagaaaatg attacgagga tacggagaga ggtatgtaca
     6421 tgtgtattta tatactaagc tgccggcggt tgtttgcaag accgagaaaa ggctagcaag
     6481 aatcgggtca ttgtagcgta tgcgcctgtg aacattctct tcaacaagtt tgattccatt
     6541 gcggtgaaat ggtaaaagtc aaccccctgc gatgtatatt ttcctgtaca atcaatcaaa
     6601 aagccaaatg atttagcatt atctttacat cttgttattt tacagatttt atgtttagat
     6661 cttttatgct tgcttttcaa aaggcctgca ggcaagtgca caaacaatac ttaaataaat
     6721 actactcagt aataacctat ttcttagcat ttttgacgaa atttgctatt ttgttagagt
     6781 cttttacacc atttgtctcc acacctccgc ttacatcaac accaataacg ccatttaatc
     6841 taagcgcatc accaacattt tctggcgtca gtccaccagc taacataaaa tgttgcctcg
     6901 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
     6961 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     7021 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct
     7081 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     7141 gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga
     7201 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7261 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7321 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7381 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7441 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7501 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7561 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7621 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7681 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7741 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7801 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     7861 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     7921 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     7981 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8041 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8101 gtaaacttgg tctgacagga gctcgctcag aagaactcgt caagaaggcg atagaaggcg
     8161 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     8221 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     8281 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     8341 ggcaagcagg catcgccatg ggtcacgacg agatcctcgc cgtcgggcat ccgcgccttg
     8401 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     8461 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     8521 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     8581 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     8641 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     8701 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt ggagttcatt cagggcaccg
     8761 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     8821 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     8881 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
     8941 gtctcttgat cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc
     9001 cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt
     9061 tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct
     9121 acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc
     9181 atccgggcga tcgatggaag ccggcggcac ctcgctaacg gattcaccac tccaagaatt
     9241 ggagccaatc aattcttgcg gagaactgtg aatgcgcaaa ccaacccttg gcagaacata
     9301 tccatcgcgt ccgccatctc cagcagccgc acgcggcgca tcgggggggg gggkkggktt
     9361 caattcatca tttttttttt attctttttt ttgatttcgg tttccttgaa atttttttga
     9421 ttcggtaatc tccgaacaga aggaagaacg aaggaaggag cacagactta gattggtata
     9481 tatacgcata tgtagtgttg aagaaacatg aaattgccca gtattcttaa cccaactgca
     9541 cagaacaaaa acctgcagga aacgaagata aatcatgtcg aaagctacat ataaggaacg
     9601 tgctgctact catcctagtc ctgttgctgc caagctattt aatatcatgc acgaaaagca
     9661 aacaaacttg tgtgcttcat tggatgttcg taccaccaag gaattactgg agttagttga
     9721 agcattaggt cccaaaattt gtttactaaa aacacatgtg gatatcttga ctgatttttc
     9781 catggagggc acagttaagc cgctaaaggc attatccgcc aagtacaatt ttttactctt
     9841 cgaagacaga aaatttgctg acattggtaa tacagtcaaa ttgcagtact ctgcgggtgt
     9901 atacagaata gcagaatggg cagacattac gaatgcacac ggtgtggtgg gcccaggtat
     9961 tgttagcggt ttgaagcagg cggcagaaga agtaacaaag gaacctagag gccttttgat
    10021 gttagcagaa ttgtcatgca agggctccct atctactgga gaatatacta agggtactgt
    10081 tgacattgcg aagagcgaca aagattttgt tatcggcttt attgctcaaa gagacatggg
    10141 tggaagagat gaaggttacg attggttgat tatgacaccc ggtgtgggtt tagatgacaa
    10201 gggagacgca ttgggtcaac agtatagaac cgtggatgat gtggtctcta caggatctga
    10261 cattattatt gttggaagag gactatttgc aaagggaagg gatgctaagg tagagggtga
    10321 acgttacaga aaagcaggct gggaagcata tttgagaaga tgcggccagc aaaactaaaa
    10381 aactgtatta taagtaaatg catgtatact aaactcacaa attagagctt caatttaatt
    10441 atatcagtta ttacccggga atctcggtcg taatgatttt tataatgacg aaaaaaaaaa
    10501 aattggaaag aaaacccccc cccccgcagc gttgggtcct ggccacgggt gcgcatgatc
    10561 gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat
    10621 gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga
    10681 gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca
    10741 gcgccctgca ccattatgtt ccggatctgc atcgcaggat gctgctggct accctgtgga
    10801 acacctacat ctgtattaac gaagcgctgg cattgaccct gagtgatttt tctctggtcc
    10861 cgccgcatcc ataccgccag ttgtttaccc tcacaacgtt ccagtaaccg ggcatgttca
    10921 tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat tacccccatg
    10981 aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac cgcccttaac
    11041 atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa cgagctggac
    11101 gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga gctttaccgc
    11161 tgggccattc tcatgaagaa tatcttgaat ttattgtcat atta
//
