LOCUS       sequence-422658-       12306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic integration of genes (inserted in I-SceI-site) via 
            rrn-interposon/SacB with TcR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12306)
  AUTHORS   Weihmann R, Kubicki S, Bitzenhofer NL, Domrose A, Bator I, Kirschen 
            LM, Kofler F, Funk A, Tiso T, Blank LM, Jaeger KE, Drepper T, Thies 
            S, Loeschcke A
  TITLE     The modular pYT vector series employed for chromosomal gene 
            integration and expression to produce carbazoles and glycolipids in 
            P. putida.
  JOURNAL   FEMS Microbes. 2022 Dec 19;4:xtac030. doi: 10.1093/femsmc/xtac030. 
            eCollection 2023.
  PUBMED    37333445
REFERENCE   2  (bases 1 to 12306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbes."; date: "2022-12-19"; volume: "4:xtac030. doi"; pages: " 
            10.1093/femsmc/xtac030. eCollection 2023"
COMMENT     Sequence Label: pYTRW28K_0Ti1
FEATURES             Location/Qualifiers
     source          1..12306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(2317..2322,2323..2339,2340..2345)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             2393..3583
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(2745..2764)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    4723..5015
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      5663..6116
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(6144..6166)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6252..6392
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6307..6326
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6407..6424)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6578..7166)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6658..6677)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7345..8139)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7456..7475)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8066..8085
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        8577..8792
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             8793..9596
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        10438..10883
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(10884..10970,10971..12305)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gagacgccac gcgatcgaac
     1261 gtcgttaact ctggcaggca attaaaggga acgtgtatct gacgcaaaga aaactggaaa
     1321 ttggcgagag aatcttctct ctgtctatcg aagactgacc acgcggaggc gtgcgtcttg
     1381 ttagcgtgcg gggtactctt gctatccatt tgagtcacac gacactcgct gttctcgact
     1441 ttacacttgt aagcgacggt attgaacgac gcttctgccc accatcgtta caaccagact
     1501 gatactagat ctatactgtc agccattcag acgaaaccag ccggagatta ccgagcactc
     1561 tattacgtcg gcgaccacta ctgagctagt ggagccgtgg gtaacgatca taccactaag
     1621 aacctctctc gacgcaagcg attacactgc tgtcacatca taatcgtctt gctattcacg
     1681 agttgaccag acactggata gcttgtcact tgaagtattc tgcacgacag ggtgcgtgta
     1741 cctatctgca gcactcaaca gatctccgtc tacttgcgac acctctctta aggtagctgg
     1801 accggattcg ggtctagcct atatcgtcaa tccgttgatc tatgtcgggt gcggagaaag
     1861 aggtaatgaa atgggcgatc gcccacagct tacacagacc taatcgtaga gttcctagta
     1921 atacttagtg gctcgttacc tcgttcttcc aggccagggg gccgtacgcg ctcggcgaac
     1981 agtataagca gattcgactc actagggata acagggtaat ctctgggtat cgactcaatc
     2041 tcgcgctatt gtgctatatc cgtggacggt tgcggtcaat tctgtcggaa aatcaacgga
     2101 tacgaacaat tactcgcgac ttcctcaata cgtgctgcaa cgcgcgcgta actataacgg
     2161 tcctaaggta gcgagcttac ttgtgctgtt gtgtaaggac acgcttaacc cagagaattc
     2221 cagcgaccta ttctacgact acgcgtccat ggctcgagga gctcggtacc gccccactac
     2281 ccgatttatc aagatttggc ggagagtccc tcatgtttga cagcttatca tcgataagct
     2341 ttaatgcggt agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc
     2401 taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt
     2461 ggttatgccg gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gcatcgccag
     2521 tcactatggc gtgctgctag cgctatatgc gttgatgcaa tttctatgcg cacccgttct
     2581 cggagcactg tccgaccgct ttggccgccg cccagtcctg ctcgcttcgc tacttggagc
     2641 cactatcgac tacgcgatca tggcgaccac acccgtcctg tggatcctct acgccggacg
     2701 catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata tcgccgacat
     2761 caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg
     2821 tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc atgcaccatt
     2881 ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc taatgcagga
     2941 gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     3001 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     3061 actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg
     3121 gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca
     3181 agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg
     3241 catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc
     3301 cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat
     3361 gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc tcgcggctct
     3421 taccagccta acttcgatca ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc
     3481 gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg tctgcctccc
     3541 cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgagtcgact ggcaaacagc
     3601 tattatgggt attatgggtg tgtatccaga acgacggatc ttgacgcaat ttggcgtggc
     3661 tctcttaagg tagccctccg accaattgag gtgcgctctg accgatcagt ttagggtgag
     3721 ccaacccggc cgatccggag agacgggcct caaagccgcg tgacgacggc tgtgggcccg
     3781 taacaaaatc cccgcaataa gctcccgtga gcgtcggttg aacagccctg gtcggcccca
     3841 tcagtagccc gaatacttcg ctttacgggt cctgggccgg ggtgcgatac cttgcagaaa
     3901 tcgaggccgt tcgttaattc ctgttgcatt cgtaccgcct atatttgtct ctttgccggc
     3961 ttatcatcac aagcatagca tagccattta tcggagcgcc tccgtacacg gtatgatcgg
     4021 acgcctcgtg agatcaatac gtataccagg tgtcctgtga gcagcgaaag cctatacgcg
     4081 agatacactg ccaaaaatcc gcgtgattac gagtcgtggc aaatttggtc tggctgtggt
     4141 ctagacattc caggcggtgc gtctgctctc gggtgcctct agtggctggc tagatagact
     4201 agccgctggt aaactggtgt ggaagtccat atatcggtga tcaatatagt ggttgacatg
     4261 ctggctagtc aacattgagc cttttgatca tgcaaatata ttacggtatt ttacaatcaa
     4321 atatcaaact taactattga ctttataact tatttaggtg gtaacattct tataaaaaag
     4381 aaaaaaatta ctgcaaaaca gtactagctt ttaacttgta tcctaggtta tctatgctgt
     4441 ctcaccatag agaatattac ctatttcaga atgtatgtcc atgattcgcc gggtaaatac
     4501 atataataca caaatctggc ttaataaagt ctataatata tctcataaag aagtgctaaa
     4561 ttggctagtg ctatatattt ttaagaaaat ttcttttgac taagtccata tcgactttgt
     4621 aaaagttcac attagcatac atatattaca cgagccagaa atagtaactt ttgcctaaat
     4681 cacaaattgc aaaatttaat tgcttgcaaa aggtcacatg cttataatca acttttttaa
     4741 aaatttaaaa tactttttta ttttttattt ttaaacataa atgaaataat ttatttattg
     4801 tttatgatta ccgaaacata aaacctgctc aagaaaaaga aactgttttg tccttggaaa
     4861 aaaagcacta cctaggagcg gccaaaatgc cgaggctttc atagcttaaa ctctttacag
     4921 aaaataggca ttatagatca gttcgagttt tcttattctt ccttccggtt ttatcgtcac
     4981 agttttacag taaataagta tcacctctta gagttaacct atactaaaat ttatgccagt
     5041 taactatgag ataagcaagt atcatctcat ttcattacct gaagtcgagt aaacagaaaa
     5101 tccaattgtt gatgaacctc aatgacttag aactatctat cggcagatca tataaagagg
     5161 atttaggtac tcgagaacgc tccgtacatg cagctaaact tccaagcatc tctaacttgg
     5221 gtagtgcttt ctccaccatt ttctgtgctt gctccttcgt ggcaagtcca gcccataatg
     5281 cccagaatgt agttgcggat tcgtatgacg ttctgtgctt gatttttgtg ttgtagtcaa
     5341 agaaaaggtc cggttaaacg gatctcgcat tgatgaggca acgctaatta tcaacatata
     5401 gattgttatc tatctgcatg aacacgaaat ctttacttga cgacttgagg ctgatggtgt
     5461 ttatgcaaag aaaccactgt gtttaatatg tgtcactgtt tgatattact gtcagcgtag
     5521 aagataatag taaaagcggt taataagtgt atttgagata agtgtgataa agtttttaca
     5581 gcgaaaagac gataaataca agaaaatgat tacgaggata cggagagagg tatgtacatg
     5641 tgtatttata tactaagctg ccggcggttg tttgcaagac cgagaaaagg ctagcaagaa
     5701 tcgggtcatt gtagcgtatg cgcctgtgaa cattctcttc aacaagtttg attccattgc
     5761 ggtgaaatgg taaaagtcaa ccccctgcga tgtatatttt cctgtacaat caatcaaaaa
     5821 gccaaatgat ttagcattat ctttacatct tgttatttta cagattttat gtttagatct
     5881 tttatgcttg cttttcaaaa ggcctgcagg caagtgcaca aacaatactt aaataaatac
     5941 tactcagtaa taacctattt cttagcattt ttgacgaaat ttgctatttt gttagagtct
     6001 tttacaccat ttgtctccac acctccgctt acatcaacac caataacgcc atttaatcta
     6061 agcgcatcac caacattttc tggcgtcagt ccaccagcta acataaaatg ttgcctcgcg
     6121 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     6181 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     6241 gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta
     6301 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     6361 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     6421 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6481 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6541 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6601 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6661 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6721 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6781 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6841 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6901 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6961 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     7021 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7081 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7141 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7201 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7261 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7321 aaacttggtc tgacaggagc tcgctcagaa gaactcgtca agaaggcgat agaaggcgat
     7381 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     7441 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     7501 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     7561 caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatcc gcgccttgag
     7621 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     7681 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     7741 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     7801 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     7861 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     7921 cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgg agttcattca gggcaccgga
     7981 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     8041 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     8101 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt
     8161 ctcttgatca gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca
     8221 gtttactttg cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc
     8281 gcttgctgtc cataaaaccg cccagtctag ctatcgccat gtaagcccac tgcaagctac
     8341 ctgctttctc tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat
     8401 ccgggcgatc gatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg
     8461 agccaatcaa ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc
     8521 catcgcgtcc gccatctcca gcagccgcac gcggcgcatc gggggggggg gggggtttca
     8581 attcatcatt ttttttttat tctttttttt gatttcggtt tccttgaaat ttttttgttt
     8641 cggtaatctc cgaacagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata
     8701 tacgcatatg tagtgttgaa gaaacatgaa attgcccagt attcttaacc caactgcaca
     8761 gaacaaaaac ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat aaggaacgtg
     8821 ctgctactca tcctagtcct gttgctgcca agctatttaa tatcatgcac gaaaagcaaa
     8881 caaacttgtg tgcttcattg gatgttcgta ccaccaagga attactggag ttagttgaag
     8941 cattaggtcc caaaatttgt ttactaaaaa cacatgtgga tatcttgact gatttttcca
     9001 tggagggcac agttaagccg ctaaaggcat tatccgccaa gtacaatttt ttactcttcg
     9061 aagacagaaa atttgctgac attggtaata cagtcaaatt gcagtactct gcgggtgtat
     9121 acagaatagc agaatgggca gacattacga atgcacacgg tgtggtgggc ccaggtattg
     9181 ttagcggttt gaagcaggcg gcagaagaag taacaaagga acctagaggc cttttgatgt
     9241 tagcagaatt gtcatgcaag ggctccctat ctactggaga atatactaag ggtactgttg
     9301 acattgcgaa gagcgacaaa gattttgtta tcggctttat tgctcaaaga gacatgggtg
     9361 gaagagatga aggttacgat tggttgatta tgacacccgg tgtgggttta gatgacaagg
     9421 gagacgcatt gggtcaacag tatagaaccg tggatgatgt ggtctctaca ggatctgaca
     9481 ttattattgt tggaagagga ctatttgcaa agggaaggga tgctaaggta gagggtgaac
     9541 gttacagaaa agcaggctgg gaagcatatt tgagaagatg cggccagcaa aactaaaaaa
     9601 ctgtattata agtaaatgca tgtatactaa actcacaaat tagagcttca atttaattat
     9661 atcagttatt acccgggaat ctcggtcgta atgattttta taatgacgaa aaaaaaaaaa
     9721 ttggaaagaa aacccccccc cccgcagcgt tgggtcctgg ccacgggtgc gcatgatcgt
     9781 gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc cttactggtt agcagaatga
     9841 atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc aaaacgtctg cgacctgagc
     9901 aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt ctggaaacgc ggaagtcagc
     9961 gccctgcacc attatgttcc ggatctgcat cgcaggatgc tgctggctac cctgtggaac
    10021 acctacatct gtattaacga agcgctggca ttgaccctga gtgatttttc tctggtcccg
    10081 ccgcatccat accgccagtt gtttaccctc acaacgttcc agtaaccggg catgttcatc
    10141 atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc ggtatcatta cccccatgaa
    10201 cagaaatccc ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat
    10261 ggcccgcttt atcagaagcc agacattaac gcttctggag aaactcaacg agctggacgc
    10321 ggatgaacag gcagacatct gtgaatcgct tcacgaccac gctgatgagc tttaccgctg
    10381 ggccattctc atgaagaata tcttgaattt attgtcatat tactagcacg tgcccatcac
    10441 atatacctgc cgttcactat tatttagtga aatgagatat tatgatattt tctgaattgt
    10501 gattaaaaag gcaactttat gcccatgcaa cagaaactat aaaaaataca gagaatgaaa
    10561 agaaacagat agatttttta gttctttagg cccgtagtct gcaaatcctt ttatgatttt
    10621 ctatcaaaca aaagaggaaa atagaccagt tgcaatccaa acgagagtct aatagaatga
    10681 ggtcgaaaag taaatcgcgc gggtttgtta ctgataaagc aggcaagacc taaaatgtgt
    10741 aaagggcaaa gtgtatactt tggcgtcacc ccttacatat tttaggtctt tttttattgt
    10801 gcgtaactaa cttgccatct tcaaacagga gggctggaag aagcagaccg ctaacacagt
    10861 acataaaaaa ggagacatga acgatgaaca tcaaaaagtt tgcaaaacaa gcaacagtat
    10921 taacctttac taccgcactg ctggcaggag gcgcaactca agcgtttgcg aaagaaacga
    10981 accaaaagcc atataaggaa acatacggca tttcccatat tacacgccat gatatgctgc
    11041 aaatccctga acagcaaaaa aatgaaaaat atcaagtttc tgaatttgat tcgtccacaa
    11101 ttaaaaatat ctcttctgca aaaggcctgg acgtttggga cagctggcca ttacaaaacg
    11161 ctgacggcac tgtcgcaaac tatcacggct accacatcgt ctttgcatta gccggagatc
    11221 ctaaaaatgc ggatgacaca tcgatttaca tgttctatca aaaagtcggc gaaacttcta
    11281 ttgacagctg gaaaaacgct ggccgcgtct ttaaagacag cgacaaattc gatgcaaatg
    11341 attctatcct aaaagaccaa acacaagaat ggtcaggttc agccacattt acatctgacg
    11401 gaaaaatccg tttattctac actgatttct ccggtaaaca ttacggcaaa caaacactga
    11461 caactgcaca agttaacgta tcagcatcag acagctcttt gaacatcaac ggtgtagagg
    11521 attataaatc aatctttgac ggtgacggaa aaacgtatca aaatgtacag cagttcatcg
    11581 atgaaggcaa ctacagctca ggcgacaacc atacgctgag agatcctcac tacgtagaag
    11641 ataaaggcca caaatactta gtatttgaag caaacactgg aactgaagat ggctaccaag
    11701 gcgaagaatc tttatttaac aaagcatact atggcaaaag cacatcattc ttccgtcaag
    11761 aaagtcaaaa acttctgcaa agcgataaaa aacgcacggc tgagttagca aacggcgctc
    11821 tcggtatgat tgagctaaac gatgattaca cactgaaaaa agtgatgaaa ccgctgattg
    11881 catctaacac agtaacagat gaaattgaac gcgcgaacgt ctttaaaatg aacggcaaat
    11941 ggtacctgtt cactgactcc cgcggatcaa aaatgacgat tgacggcatt acgtctaacg
    12001 atatttacat gcttggttat gtttctaatt ctttaactgg cccatacaag ccgctgaaca
    12061 aaactggcct tgtgttaaaa atggatcttg atcctaacga tgtaaccttt acttactcac
    12121 acttcgctgt acctcaagcg aaaggaaaca atgtcgtgat tacaagctat atgacaaaca
    12181 gaggattcta cgcagacaaa caatcaacgt ttgcgccgag cttcctgctg aacatcaaag
    12241 gcaagaaaac atctgttgtc aaagacagca tccttgaaca aggacaatta acagttaaca
    12301 aataaa
//
