LOCUS       sequence-422660-       12502 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12502)
  AUTHORS   Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM
  TITLE     The Brd4 Extraterminal Domain Confers Transcription Activation 
            Independent of pTEFb by Recruiting Multiple Proteins, Including 
            NSD3.
  JOURNAL   Mol Cell Biol. 2011 Jul . 31(13):2641-52.
  PUBMED    21555454
REFERENCE   2  (bases 1 to 12502)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2011 Jul . 31(13):2641-52."
COMMENT     Sequence Label: p6350 MSCV-CMV-CMV-Flag-HA-ATAD5
FEATURES             Location/Qualifiers
     source          1..12502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..517
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    581..922
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             989..1405
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1335..1357
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1373..1389
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1420..1799
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1800..2003
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1954..1974
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2005..2023
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2026..2044
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2054..2078
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2151..2160
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2160..2183
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2196..2222
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2238..2262
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2296..2305
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(7825..7849)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        7868..8367
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7903..7926)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8300..8319
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8388..8987
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8388..8407)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8884..8904
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    9573..9589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9597..9603,9604..9621,9622..9627))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9642..9663
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9780..9797)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9951..10539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10031..10050)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10710..11501,11502..11570))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11333..11352
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11571..11675)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11743..11761
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11799..11821)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11921..11940
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12134..12156
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12284..12303
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
      121 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      181 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
      241 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
      301 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
      361 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa
      421 taaagcctct tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct
      481 cagattgatt gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga
      541 gacccctgcc cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt
      601 cgtgtctgtc tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct
      661 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg
      721 aacacccggc cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc
      781 gacctgagga agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg
      841 agacgagaac ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg
      901 aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac
      961 tgtgtttctg tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac
     1021 cttaggtcac tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa
     1081 gagacgttgg gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg
     1141 agacggcacc tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg
     1201 cccgcatgga cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga
     1261 cccccctccc tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc
     1321 cgccccgtct ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc
     1381 agccctcact ccttctctag gcgccggaat tagatccttg acattgatta ttgactagtt
     1441 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     1501 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     1561 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     1621 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     1681 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     1741 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     1801 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     1861 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     1921 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     1981 gggaggtcta tataagcaga gctctcccta tcagtgatag agatctccct atcagtgata
     2041 gagatcgtcg acgagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     2101 gttttgacct ccatagaaga caccgggacc gatccagcct ccggggatct gccaccatgg
     2161 actacaagga tgacgatgac aagctcgatg gaggataccc atacgatgtt ccagattacg
     2221 ctgctcgagg ttatcaaaca agtttgtaca aaaaagcaga agggccgtca aggcccacca
     2281 tggtgggggt cctggccatg gcggctgcag ctgctccgcc tcccgtgaag gactgcgaga
     2341 ttgagccatg caaaaagcga aagaaagatg atgacacatc tacctgcaaa acaattacaa
     2401 aatatttatc accactaggg aagactagag acagggtttt tgctccacca aaacctagta
     2461 atattctgga ttattttaga aagacttcac ccacaaatga gaagacacaa ttagggaaag
     2521 agtgcaagat aaagtcacct gaatcagtac ctgttgacag caacaaagac tgtacgacac
     2581 ctttggaaat gttctcaaat gtagagttta agaagaaaag aaagagggtt aatttatctc
     2641 atcaactaaa taatattaaa actgaaaatg aagctccaat tgaaattagt agcgacgata
     2701 gcaaagaaga ctatagttta aataatgatt ttgtggaaag tagtacttct gttttacgtt
     2761 acaagaaaca agtagaggta cttgcagaaa acattcaaga tacaaaaagt caaccaaata
     2821 ctatgacctc cctgcaaaat tctaaaaaag taaatcctaa acaagggacc acaaaaaatg
     2881 acttcaaaaa gttgagaaaa aggaaatgca gagatgtagt agatctatct gaaagcttac
     2941 ccttggcaga ggaactaaat ttgcttaaaa aagatggtaa agatactaaa cagatggaga
     3001 atactacaag ccatgcaaac tctagagata acgtaactga agcagcccag ttaaatgata
     3061 gtataataac tgtctcatat gaggaatttt taaaaagtca caaggaaaat aaagtggaag
     3121 agataccaga ctctacaatg tcaatttgtg ttccttctga aactgtcgac gaaatagtca
     3181 aaagtggtta tataagtgaa tcagaaaact ccgaaatttc ccagcaggta cgctttaaga
     3241 cagttactgt tcttgcacag gttcacccta ttccgcccaa aaagacaggg aaaatacccc
     3301 gaattttctt gaaacaaaag caatttgaaa tggaaaatag tttatctgat cctgagaatg
     3361 aacagacagt tcagaaaaga aaatctaatg ttgttataca ggaggaagaa ttagaattgg
     3421 ctgttttgga agctggaagt tctgaagctg tgaaaccaaa atgcactcta gaagaaagac
     3481 agcaatttat gaaagcattt aggcagccag catcagatgc acttaaaaat ggagttaaaa
     3541 agtcttctga taagcagaaa gaccttaatg aaaaatgtct atatgaagta ggaagagatg
     3601 ataattctaa aaaaatcatg gaaaattctg gtatccaaat ggtttcaaaa aatggcaatt
     3661 tacagttaca cactgataaa ggaagttttc tgaaggagaa aaataaaaag ctaaagaaga
     3721 agaataagaa aacattagat actggggcta ttccaggcaa aaacagagag ggaaacactc
     3781 aaaagaaaga aacaaccttt ttcttaaaag agaaacaata tcaaaataga atgagtttaa
     3841 gacaaaggaa aacagagttt ttcaaaagca gcactttatt taacaatgaa agtcttgttt
     3901 atgaagatat agcaaatgat gaccttctaa aggtttcctc tctgtgtaac aataataaat
     3961 tgtcaagaaa aaccagcata ccagttaaag atattaagct tacacagtct aaagctgaat
     4021 ctgaagccag cttgctaaat gtttccacgc ccaagtcaac tagaagatct ggaagaatta
     4081 gcagcacacc tactacagaa accattagag gtattgattc tgacgatgta caagataata
     4141 gtcaactaaa ggcttccact caaaaagcag ccaacttatc ggaaaagcac agcttatata
     4201 cagcagaatt aataacagta ccctttgatt cagagagccc tattagaatg aaattcacca
     4261 gaattagtac tcccaaaaaa tctaagaaaa aatctaacaa aagatctgag aaatctgaag
     4321 caactgatgg aggttttact tctcagatta gaaaggcaag caatacttca aaaaacatat
     4381 caaaagcaaa acaattgatt gaaaaagcaa aagctttaca catcagtagg tcaaaggtga
     4441 ctgaagaaat agcgataccc ttaaggcgct cctctagaca tcagacactt cctgaaagga
     4501 agaaattgtc agaaacagaa gattctgtta taataataga ttcaagtcct actgctttaa
     4561 agcatccaga gaaaaatcag aagaaacttc agtgtttgaa tgatgtgcta ggaaaaaaac
     4621 ttaacacatc cactaaaaat gtacctggaa aaatgaaagt cgctccttta tttcttgtca
     4681 gaaaagcaca aaaagcagct gatcctgtcc ctagttttga tgaaagcagt caagatacat
     4741 ctgaaaaatc tcaggattgt gatgttcaat gtaaagcaaa gcgtgacttc ctaatgagtg
     4801 gtttgccaga tttgttgaaa cggcaaattg caaagaaagc tgctgcgctg gatgtgtaca
     4861 atgcagtgag taccagtttc cagagagtcg tacatgtgca acaaaaggat gatgggtgtt
     4921 gtttgtggca tttgaaacca ccctcttgtc ctctcttaac taaatttaaa gaactgaaca
     4981 ctaaagtaat agatctctca aaatgtggta ttgctcttgg tgaattttca acattgaatt
     5041 caaagttgaa aagcggtaac tctgctgctg tgttcatgag gacaaggaag gaatttactg
     5101 aagaagtaag aaatcttttg cttgaggaaa ttaggtggtc aaatcctgaa ttttcattga
     5161 aaaaatattt tcccttactc ctaaaaaaac aaattgagca ccaagtactt tcttccgagt
     5221 gtcatagtaa acaagaactg gaggctgatg tcagccataa agaaaccaaa aggaaactcg
     5281 tagaagcaga aaattctaag tcaaaaagaa agaaaccaaa tgagtattca aaaaatctgg
     5341 agaagaccaa taggaagtca gaagaactta gcaaaagaaa caactcttct gggataaagc
     5401 tagattcttc caaagattct ggaactgaag acatgctttg gacagaaaag tatcaacctc
     5461 agactgccag tgaacttata ggaaatgagt tagctataaa aaagttacat agttggttga
     5521 aagactggaa aagaagagct gaattggaag aaaggcagaa tctgaaggga aaaagagatg
     5581 agaaacatga agatttctcg ggtggcatag actttaaagg cagttcagat gatgaagaag
     5641 agagtcgtct ttgcaatact gtccttataa cagggccaac aggagtggga aaaactgctg
     5701 cagtgtatgc ttgtgcccag gagcttggat ttaagatatt tgaagtgaat gcctcttccc
     5761 agcgcagtgg tagacaaatt ctatctcagt tgaaggaagc tactcagtcc catcaagtag
     5821 acaaacaagg tgtaaactca caaaaaccct gtttttttaa tagctactac ataggcaagt
     5881 caccaaaaaa aataagctcc cctaagaaag ttgttacatc accaagaaaa gttcctccac
     5941 catcaccaaa aagtagtgga ccaaagcgag cacttcctcc caaaaccttg gcaaattatt
     6001 ttaaagtatc tcccaaacct aaaaataatg aagaaatagg aatgcttctg gaaaataata
     6061 aaggaataaa aaattctttt gaacagaaac aaattactca gactaaatct acaaatgcaa
     6121 ctaattcaaa tgtcaaagac gttggagctg aagaacccag cagaaaaaat gcaacatctc
     6181 ttattctttt tgaggaggtt gatgtaattt ttgatgaaga tgctgggttt ttgaatgcaa
     6241 tcaaaacatt catggcaaca actaaacgac ctgtaatcct tactacaagt gacccaacat
     6301 ttagtttaat gtttgatggc tgctttgaag aaatcaagtt cagtactcct tccctgctaa
     6361 atgttgccag ctacctacaa atgatttgct taactgagaa ttttagaact gatgtaaaag
     6421 actttgtaac cttgttaact gcaaatactt gtgatatcag aaaaagtatc ctttacttac
     6481 aattctggat tagaagtgga ggtggagttt tagaagaacg accattaacc ctttatcgtg
     6541 gaaatagcag aaatgtacaa ctagtttgct ctgaacatgg ccttgataac aaaatttacc
     6601 ctaaaaatac taaaaagaaa cgtgtagacc ttccaaaatg tgacagtggc tgtgctgaga
     6661 ccttgtttgg ccttaagaac attttttccc catctgaaga cttattttca tttttaaagc
     6721 acaaaatcac aatgaaggaa gaatggcata aattcatcca gcttcttaca gaattccaaa
     6781 tgcggaatgt agatttttta tatagtaatc ttgagtttat tctaccatta ccagttgata
     6841 ccattccaga aactaaaaac ttttgtggcc catcagtaac tgtggatgcc agtgcagcaa
     6901 caaaaagtat gaattgtctt gctaggaaac actctgaaag agaacagcca ttgaaaaagt
     6961 cccagaaaaa gaaacaaaag aaaacattgg taatattaga tgatagtgat ctatttgaca
     7021 ctgacttgga ctttcctgat caatctatta gcctgtcctc tgtatcatct tcctcaaatg
     7081 cagaagaaag caaaaccgga gacgaagaaa gcaaagccag agacaaagga aacaatccag
     7141 agacaaagaa atctattcct tgtcctccta aaacaactgc aggaaaaaaa tgttctgccc
     7201 ttgtttctca ttgtttaaat tctctctctg agttcatgga taacatgtcc ttcttagatg
     7261 cacttttaac tgatgtaagg gaacaaaaca aatacggtag aaatgacttt agttggacaa
     7321 atggaaaggt tacaagtgga ctttgtgatg agtttagtct tgagagtaat gatggatgga
     7381 cttctcaaag ctctggagaa ttaaaggcag ctgcagaagc tctcagcttt actaaatgtt
     7441 cttctgctat ttcaaaagca ttggaaacct tgaattcttg caagaaatta ggaagagatc
     7501 caaccaacga tcttactttt tatgtttcac aaaagcgcaa taatgtatac tttagtcagt
     7561 cagcagctaa tttagacaat gcttggaaga ggatatcagt cattaaaagt gtattttcga
     7621 gtcgatctct tctctatgtg ggtaatagac aagctagtat aattgaatac ctgccaaccc
     7681 ttcgaaacat ctgtaagact gagaagctaa aagaacaagg aaaaagtaaa agaagattcc
     7741 tgcactattt tgaaggaatt catcttgaca ttccaaaaga gactgtgaat actttggcag
     7801 ctgacttccc ttcaggcctc atgggcccag ctttcttgta caaagtggtt cgataacgaa
     7861 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     7921 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     7981 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     8041 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     8101 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     8161 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     8221 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     8281 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     8341 tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg accgagtaca
     8401 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     8461 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     8521 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     8581 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     8641 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     8701 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     8761 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     8821 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     8881 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     8941 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
     9001 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgataaaa taaaagattt
     9061 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     9121 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
     9181 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
     9241 aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc gtcgcccggg
     9301 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
     9361 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tgggtaacag
     9421 tttcttgaag ttggagaaca acattctgag ggtaggagtc gaatattaag taatcctgac
     9481 tcaattagcc actgttttga atccacatac tccaatactc ctgaaatagt tcattatgga
     9541 cagcgcagaa agagctgggg agaattgtga aattgttatc cgctcacaat tccacacaac
     9601 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9661 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9721 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9781 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9841 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9901 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9961 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    10021 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    10081 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10141 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10201 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10261 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10321 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10381 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10441 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10501 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10561 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10621 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10681 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10741 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10801 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10861 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10921 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10981 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    11041 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    11101 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    11161 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    11221 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    11281 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    11341 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    11401 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    11461 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    11521 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11581 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11641 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11701 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
    11761 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
    11821 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
    11881 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
    11941 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
    12001 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
    12061 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
    12121 acgccagggt tttcccagtc acgacgttgt aaaacgacgg cgcaaggaat ggtgcatgca
    12181 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
    12241 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
    12301 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
    12361 ggcgattagt ccaatttgtt aaagacagga tatcagtggt ccaggctcta gttttgactc
    12421 aacaatatca ccagctgaag cctatagagt acgagccata gataaaataa aagattttat
    12481 ttagtctcca gaaaaagggg gg
//
