LOCUS       sequence-422665-       10461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic integration of genes (inserted in I-SceI-site) via 
            transposon Tn5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Weihmann R, Kubicki S, Bitzenhofer NL, Domrose A, Bator I, Kirschen 
            LM, Kofler F, Funk A, Tiso T, Blank LM, Jaeger KE, Drepper T, Thies 
            S, Loeschcke A
  TITLE     The modular pYT vector series employed for chromosomal gene 
            integration and expression to produce carbazoles and glycolipids in 
            P. putida.
  JOURNAL   FEMS Microbes. 2022 Dec 19;4:xtac030. doi: 10.1093/femsmc/xtac030. 
            eCollection 2023.
  PUBMED    37333445
REFERENCE   2  (bases 1 to 10461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbes."; date: "2022-12-19"; volume: "4:xtac030. doi"; pages: " 
            10.1093/femsmc/xtac030. eCollection 2023"
COMMENT     Sequence Label: yTREX-ApR
FEATURES             Location/Qualifiers
     source          1..10461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1353..1371
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1444..1460)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1444..1460)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        complement(1470..1488)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        join(1544..1549,1550..1566,1567..1572)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1620..2810
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1972..1991)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    4894..5186
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      5834..6287
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(6315..6337)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6423..6563
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6478..6497
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6578..6595)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6749..7337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6829..6848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7514..8305,8306..8374))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8137..8156
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8375..8479)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8656..8871
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             8872..9675
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 gccattctca tgaagaatat cttgaattta ttgtcatatt actagtcccg ggtccacata
       61 tccacgggct ggcaagggag cgcagcgacc gcgcagggcg aagcccggag agcaagcccg
      121 tagggcgccg cagccgccgt aggcggtcac gactttgcga agcaaagtct agtgagtata
      181 ctcaagcatt gagtggcccg ccggaggcac cgccttgcgc tgcccccgtc gagccggttg
      241 gacaccaaaa gggaggggca ggcatggcgg catacgcgat catgcgatgc aagaagctgg
      301 cgaaaatggg caacgtggcg gccagtctca agcacgccta ccgcgagcgc gagacgccca
      361 acgctgacgc cagcaggacg ccagagaacg agcactgggc ggccagcagc accgatgaag
      421 cgatgggccg actgcgcgag ttgctgccag agaagcggcg caaggacgct gtgttggcgg
      481 tcgagtacgt catgacggcc agcccggaat ggtggaagtc ggccagccaa gaacagcagg
      541 cggcgttctt cgagaaggcg cacaagtggc tggcggacaa gtacggggcg gatcgcatcg
      601 tgacggccag catccaccgt gacgaaacca gcccgcacat gaccgcgttc gtggtgccgc
      661 tgacgcagga cggcaggctg tcggccaagg agttcatcgg caacaaagcg cagatgaccc
      721 gcgaccagac cacgtttgcg gccgctgtgg ccgatctagg gctgcaacgg ggcatcgagg
      781 gcagcaaggc acgtcacacg cgcattcagg cgttctacga ggccctggag cggccaccag
      841 tgggccacgt caccatcagc ccgcaagcgg tcgagccacg cgcctatgca ccgcagggat
      901 tggccgaaaa gctgggaatc tcaaagcgcg ttgagacgcc ggaagccgtg gccgaccggc
      961 tgacaaaagc ggttcggcag gggtatgagc ctgccctaca ggccgccgca ggagcgcgtg
     1021 agatgcgcaa gaaggccgat caagcccaag agacggcccg agaccttcgg gagcgcctga
     1081 agcccgttct ggacgccctg gggccgttga atcgggatat gcaggccaag gccgccgcga
     1141 tcatcaaggc cgtgggcgaa aagctgctga cggaacagcg ggaagtccag cgccagaaac
     1201 aggcccagcg ccagcaggaa cgcgggcgcg cacatttccc cgaactgact cttatacaca
     1261 agtgttcttt cctgcgttat cccctgattc tgtggataac cgtgaattcg gtttcccgac
     1321 tggaaagcgg gcagtgagcg caacgcaata attaatacga ctcactatag gcctccaaac
     1381 tagaaatatt agctaattta atctctcaac ctagggataa cagggtaatt tggcgtaatc
     1441 atggtcatag ctgtttcctg tgtgaaattc ctatagtgag tcgtattaat taggttgggc
     1501 ttcggaatcg ttttccggga cgccggctgg agaattctca tgtttgacag cttatcatcg
     1561 ataagcttta atgcggtagt ttatcacagt taaattgcta acgcagtcag gcaccgtgta
     1621 tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt caccctggat gctgtaggca
     1681 taggcttggt tatgccggta ctgccgggcc tcttgcggga tatcgtccat tccgacagca
     1741 tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt gatgcaattt ctatgcgcac
     1801 ccgttctcgg agcactgtcc gaccgctttg gccgccgccc agtcctgctc gcttcgctac
     1861 ttggagccac tatcgactac gcgatcatgg cgaccacacc cgtcctgtgg atcctctacg
     1921 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
     1981 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
     2041 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
     2101 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
     2161 tgcaggagtc gcataaggga gagcgtcgac cgatgccctt gagagccttc aacccagtca
     2221 gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact gtcttcttta
     2281 tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc gaggaccgct
     2341 ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc ttgcacgccc
     2401 tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag caggccatta
     2461 tcgccggcat ggcggccgac gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct
     2521 ggatggcctt ccccattatg attcttctcg cttccggcgg catcgggatg cccgcgttgc
     2581 aggccatgct gtccaggcag gtagatgacg accatcaggg acagcttcaa ggatcgctcg
     2641 cggctcttac cagcctaact tcgatcattg gaccgctgat cgtcacggcg atttatgccg
     2701 cctcggcgag cacatggaac gggttggcat ggattgtagg cgccgcccta taccttgtct
     2761 gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac ctcgacctga agagcgccca
     2821 atacgcaaac cgcctctccc cgcgcgttgg acttgtgtat aagagtcaga gcgtcctgaa
     2881 cggaaccttt cccgttttcc aggatctgat cttccatgtg acctcctaac atggtaacgt
     2941 tcatgataac ttctgctctt catcgtgcgg ccgactgggc taaatctgtg ttctcttcgg
     3001 cggcgctggg tgatcctcgc cgtactgccc gcttggttaa cgtcgccgcc caattggcaa
     3061 aatattctgg taaatcaata accatctcat cagagggtag tgaagccatg caggaaggcg
     3121 cttaccgatt tatccgcaat cccaacgttt ctgccgaggc gatcagaaag gctggcgcca
     3181 tgcaaacagt caagttggct caggagtttc ccgaactgct ggccattgag gacaccacct
     3241 ctttgagtta tcgccaccag gtcgccgaag agcttggcaa gctgggctct attcaggata
     3301 aatcccgcgg atggtgggtt cactccgttc tcttgctcga ggccaccaca ttccgcaccg
     3361 taggattact gcatcaggag tggtggatgc gcccggatga ccctgccgat gcggatgaaa
     3421 aggagagtgg caaatggctg gcagcggccg caactagccg gttacgcatg ggcagcatga
     3481 tgagcaacgt gattgcggtc tgtgaccgcg aagccgatat tcatgcttat ctgcaggaca
     3541 aactggcgca taacgagcgc ttcgtggtgc gctccaagca cccacgcaag gacgtagagt
     3601 ctgggttgta tctgtacgac catctgaaga accaaccgga gttgggtggc tatcagatca
     3661 gcattccgca aaagggcgtg gtggataaac gcggtaaacg taaaaatcga ccagcccgca
     3721 aggcgagctt gagcctgcgc agtgggcgca tcacgctaaa acaggggaat atcacgctca
     3781 acgcggtgct ggccgaggag attaacccgc ccaagggtga gaccccgttg aaatggttgt
     3841 tgctgaccag cgaaccggtc gagtcgctag cccaagcctt gcgcgtcatc gacatttata
     3901 cccatcgctg gcggatcgag gagttccata aggcatggaa aaccggagca ggagccgaga
     3961 ggcaacgcat ggaggagccg gataatctgg agcggatggt ctcgatcctc tcgtttgttg
     4021 cggtcaggct gttacagctc agagaaagct tcacgctgcc gcaagcactc agggcgcaag
     4081 ggctgctaaa ggaagcggaa cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg
     4141 atgaatgtca gctactgggc tatctggaca agggaaaacg caagcgcaaa gagaaagcag
     4201 gtagcttgca gtgggcttac atggcgatag ctagactggg cggttttatg gacagcaagc
     4261 gaaccggaat tgccagctgg ggcgccctct gggaaggttg ggaagccctg caaagtaaac
     4321 tggatggctt tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgacccggga
     4381 ctagttggtg tggaagtcct aatatcggtg atcaatatag tggttgacat gctggctagt
     4441 caacattgag ccttttgatc atgcaaatat attacggtat tttacaatca aatatcaaac
     4501 ttaactattg actttataac ttatttaggt ggtaacattc ttataaaaaa gaaaaaaatt
     4561 actgcaaaac agtactagct tttaacttgt atcctaggtt atctatgctg tctcaccata
     4621 gagaatatta cctatttcag aatgtatgtc catgattcgc cgggtaaata catataatac
     4681 acaaatctgg cttaataaag tctataatat atctcataaa gaagtgctaa attggctagt
     4741 gctatatatt tttaagaaaa tttcttttga ctaagtccat atcgactttg taaaagttca
     4801 cattagcata catatattac acgagccaga aatagtaact tttgcctaaa tcacaaattg
     4861 caaaatttaa ttgcttgcaa aaggtcacat gcttataatc aactttttta aaaatttaaa
     4921 atactttttt attttttatt tttaaacata aatgaaataa tttatttatt gtttatgatt
     4981 accgaaacat aaaacctgct caagaaaaag aaactgtttt gtccttggaa aaaaagcact
     5041 acctaggagc ggccaaaatg ccgaggcttt catagcttaa actctttaca gaaaataggc
     5101 attatagatc agttcgagtt ttcttattct tccttccggt tttatcgtca cagttttaca
     5161 gtaaataagt atcacctctt agagttaacc tatactaaaa tttatgccag ttaactatga
     5221 gataagcaag tatcatctca tttcattacc tgaagtcgag taaacagaaa atccaattgt
     5281 tgatgaacct caatgactta gaactatcta tcggcagatc atataaagag gatttaggta
     5341 ctcgagaacg ctccgtacat gcagctaaac ctccaagcat ctctaacttg ggtagtgctt
     5401 tctccaccat tttctgtgct tgctccttcg tggcaagtcc agcccataat gcccagaatg
     5461 tagttgcgga ttcgtatgac gttctgtgct tgatttttgt gttgtagtca aagaaaaggt
     5521 ccggttaaac ggatctcgca ttgatgaggc aacgctaatt atcaacatat agattgttat
     5581 ctatctgcat gaacacgaaa tctttacttg acgacttgag gctgatggtg tttatgcaaa
     5641 gaaaccactg tgtttaatat gtgtcactgt ttgatattac tgtcagcgta gaagataata
     5701 gtaaaagcgg ttaataagtg tatttgagat aagtgtgata aagtttttac agcgaaaaga
     5761 cgataaatac aagaaaatga ttacgaggat acggagagag gtatgtacat gtgtatttat
     5821 atactaagct gccggcggtt gtttgcaaga ccgagaaaag gctagcaaga atcgggtcat
     5881 tgtagcgtat gcgcctgtga acattctctt caacaagttt gattccattg cggtgaaatg
     5941 gtaaaagtca accccctgcg atgtatattt tcctgtacaa tcaatcaaaa agccaaatga
     6001 tttagcatta tctttacatc ttgttatttt acagatttta tgtttagatc ttttatgctt
     6061 gcttttcaaa aggcctgcag gcaagtgcac aaacaatact taaataaata ctactcagta
     6121 ataacctatt tcttagcatt tttgacgaaa tttgctattt tgttagagtc ttttacacca
     6181 tttgtctcca cacctccgct tacatcaaca ccaataacgc catttaatct aagcgcatca
     6241 ccaacatttt ctggcgtcag tccaccagct aacataaaat gttgcctcgc gcgtttcggt
     6301 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     6361 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     6421 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     6481 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     6541 taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6601 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6661 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6721 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6781 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6841 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6901 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6961 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7021 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7081 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7141 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7201 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7261 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7321 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7381 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7441 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7501 ctgacaggag ctcttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7561 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7621 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7681 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7741 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7801 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     7861 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7921 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7981 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8041 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8101 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     8161 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8221 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8281 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8341 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8401 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8461 aacaaatagg ggttccgcgc accgatcgat ggaagccggc ggcacctcgc taacggattc
     8521 accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac
     8581 ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatcggg
     8641 gggggggggg ggggtttcaa ttcatcattt tttttttttt tttttttttg ttttcggttt
     8701 ccttgaaatt tttttgtttc ggtaatctcc gaacagaagg aagaacgaag gaaggagcac
     8761 agacttagat tggtatatat acgcatatgt agtgttgaag aaacatgaaa ttgcccagta
     8821 ttcttaaccc aactgcacag aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa
     8881 gctacatata aggaacgtgc tgctactcat cctagtcctg ttgctgccaa gctatttaat
     8941 atcatgcacg aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac caccaaggaa
     9001 ttactggagt tagttgaagc attaggtccc aaaatttgtt tactaaaaac acatgtggat
     9061 atcttgactg atttttccat ggagggcaca gttaagccgc taaaggcatt atccgccaag
     9121 tacaattttt tactcttcga agacagaaaa tttgctgaca ttggtaatac agtcaaattg
     9181 cagtactctg cgggtgtata cagaatagca gaatgggcag acattacgaa tgcacacggt
     9241 gtggtgggcc caggtattgt tagcggtttg aagcaggcgg cagaagaagt aacaaaggaa
     9301 cctagaggcc ttttgatgtt agcagaattg tcatgcaagg gctccctatc tactggagaa
     9361 tatactaagg gtactgttga cattgcgaag agcgacaaag attttgttat cggctttatt
     9421 gctcaaagag acatgggtgg aagagatgaa ggttacgatt ggttgattat gacacccggt
     9481 gtgggtttag atgacaaggg agacgcattg ggtcaacagt atagaaccgt ggatgatgtg
     9541 gtctctacag gatctgacat tattattgtt ggaagaggac tatttgcaaa gggaagggat
     9601 gctaaggtag agggtgaacg ttacagaaaa gcaggctggg aagcatattt gagaagatgc
     9661 ggccagcaaa actaaaaaac tgtattataa gtaaatgcat gtatactaaa ctcacaaatt
     9721 agagcttcaa tttaattata tcagttatta cccgggaatc tcggtcgtaa tgatttttat
     9781 aatgacgaaa aaaaaaaaat tggaaagaaa accccccccc cccgcagcgt tgggtcctgg
     9841 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc
     9901 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc
     9961 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt
    10021 ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc
    10081 tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga
    10141 gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc
    10201 agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc
    10261 ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag
    10321 gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag
    10381 aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac
    10441 gctgatgagc tttaccgctg g
//
