LOCUS       sequence-422667-        5906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This binary vector expresses mNeonGreen with a truncated 35sCAMV 
            promoter with strong UTR (Tomato Mosaic Virus).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5906)
  TITLE     Curtis lab plasmids
REFERENCE   2  (bases 1 to 5906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLSU1/t35S[TMV]:mNeonGreen:NosT
FEATURES             Location/Qualifiers
     source          1..5906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..195
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     658..678
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        667..713
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      1540..1792
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1968..1987)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1979..2794
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1979..2794
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2052..2071)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2880..3468
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3369..3388
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3710..4339
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4768..5841
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatgatat cgcatgcgga aacctcctcg gattccattg
      421 cccagctatc tgtcacttta ttgagaagat agtggaaaag gaaggtggct cctacaaatg
      481 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
      541 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      601 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
      661 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
      721 gagtataaga gctcattttt acaacaatta ccaacaacaa caaacaacaa acaacattac
      781 aattacattt acaattatcg atacatcgag ggtaccgaat tcatggtttc caaaggtgag
      841 gaagataata tggctagttt acctgccaca catgagctac acatattcgg cagcataaac
      901 ggtgtagatt ttgatatggt tggacaaggg acaggtaatc ctaatgatgg atatgaggag
      961 ttgaacctca aatctaccaa gggggacctt caattctccc catggatcct agtccctcac
     1021 attggatacg gcttccacca gtacttacca tacccagatg gaatgtcccc tttccaagca
     1081 gccatggttg acggttccgg gtaccaggta cataggacaa tgcaatttga ggacggggct
     1141 tctctgactg tgaattaccg atatacctat gaaggcagtc acataaaggg cgaagcacaa
     1201 gtgaagggca ccggatttcc tgccgacggg cctgtaatga ctaatagctt gaccgccgca
     1261 gattggtgca ggtcaaaaaa gacctaccca aatgacaaga caataatctc tacatttaag
     1321 tggagctaca ctacagggaa tggtaaacga tatagatcaa ctgctagaac cacatatacc
     1381 ttcgctaaac ctatggcagc caattatctg aagaaccagc ccatgtatgt gttccgaaag
     1441 actgaactca agcatagcaa aaccgaactc aacttcaaag agtggcaaaa ggctttcact
     1501 gacgtgatgg gaatggacga gttatataag taactgcagg atcgttcaaa catttggcaa
     1561 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     1621 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     1681 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     1741 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcggggagct
     1801 ctggcaggat atatgcggtt gtaattcatt tttattgtct aaatttctgt atttgtttgt
     1861 ttgcaattag atccacgttg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     1921 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     1981 gagccatatt caacgggaaa cgtcttgttc gaggccgcga ttaaattcca acatggatgc
     2041 tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg cgacaatcta
     2101 ccgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca aaggtagcgt
     2161 tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat ttatgcctct
     2221 tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca ccactgcgat
     2281 cccagggaaa acagcattcc aggtattaga agaatatcct gattcaggtg aaaatattgt
     2341 tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta attgtccttt
     2401 taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata acggtttggt
     2461 tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag tctggaaaga
     2521 aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg atttctcact
     2581 tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg gacgagtcgg
     2641 aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg agttttctcc
     2701 ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata tgaataaatt
     2761 gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt ggttgtaaca
     2821 ctggcagagc attacgctga cttgacggga cggcggcttt gttgaatcaa ttgatcttct
     2881 tgaaatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     2941 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     3001 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     3061 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     3121 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     3181 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     3241 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     3301 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     3361 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     3421 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     3481 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     3541 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgatctct gaagaaaccg
     3601 agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg
     3661 ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat
     3721 cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg
     3781 cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc
     3841 ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc
     3901 gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc
     3961 gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc
     4021 ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca
     4081 gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa
     4141 aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct
     4201 tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt
     4261 tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat
     4321 taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg
     4381 ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca
     4441 gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg
     4501 aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg
     4561 cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg
     4621 aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg
     4681 aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac
     4741 tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa
     4801 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag
     4861 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga
     4921 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc
     4981 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat
     5041 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc
     5101 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc
     5161 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
     5221 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
     5281 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
     5341 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
     5401 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
     5461 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagttgattg gatgtaccgc
     5521 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
     5581 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
     5641 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
     5701 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
     5761 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
     5821 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
     5881 gaaaaaggtc gaaaagatct ctttcc
//
