LOCUS       sequence-422668-        5629 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This binary vector has an empty backbone where you can insert a gene
            into a cassette with a strong promoter (original 35sCAMV).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5629)
  TITLE     Curtis lab plasmids
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLSU1/o35S[]:MCS:NosT
FEATURES             Location/Qualifiers
     source          1..5629
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..195
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        885..1230
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1173..1193
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      1263..1515
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1691..1710)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1702..2517
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1702..2517
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1775..1794)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2603..3191
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3092..3111
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3433..4062
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4491..5564
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatgatat cgcatgcaga ttagcctttt caatttcaga
      421 aagaatgcta acccacagat ggttagagag gcttacgcag caggtctcat caagacgatc
      481 tacccgagca ataatctcca ggaaatcaaa taccttccca agaaggttaa agatgcagtc
      541 aaaagattca ggactaactg catcaagaac acagagaaag atatatttct caagatcaga
      601 agtactattc cagtatggac gattcaaggc ttgcttcaca aaccaaggca agtaatagag
      661 attggagtct ctaaaaaggt agttcccact gaatcaaagg ccatggagtc aaagattcaa
      721 atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca gagtctctta
      781 cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac acttgtctac
      841 tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caattgagac ttttcaacaa
      901 agggtaatat ccggaaacct cctcggattc cattgcccag ctatctgtca ctttattgtg
      961 aagatagtgg aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggcc
     1021 atcgttgaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     1081 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgatatc
     1141 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     1201 taaggaagtt catttcattt ggagagaaca cgctcgaggg taccgaattc ggatccctgc
     1261 aggatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     1321 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     1381 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     1441 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     1501 ctatgttact agatcgggga gctctggcag gatatatgcg gttgtaattc atttttattg
     1561 tctaaatttc tgtatttgtt tgtttgcaat tagatccacg ttgtgtctca aaatctctga
     1621 tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata
     1681 aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcttg ttcgaggccg
     1741 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
     1801 gggcaatcag gtgcgacaat ctaccgattg tatgggaagc ccgatgcgcc agagttgttt
     1861 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     1921 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     1981 gcatggttac tcaccactgc gatcccaggg aaaacagcat tccaggtatt agaagaatat
     2041 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     2101 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     2161 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     2221 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     2281 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     2341 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     2401 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     2461 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     2521 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcggc
     2581 tttgttgaat caattgatct tcttgaaatc ctttttttct gcgcgtaatc tgctgcttgc
     2641 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     2701 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     2761 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     2821 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     2881 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     2941 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3001 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3061 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3121 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3181 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3241 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3301 ttgagtgatc tctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     3361 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     3421 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     3481 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     3541 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     3601 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     3661 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     3721 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
     3781 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
     3841 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
     3901 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
     3961 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
     4021 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
     4081 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
     4141 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
     4201 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
     4261 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
     4321 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
     4381 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
     4441 gtctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
     4501 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
     4561 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
     4621 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
     4681 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
     4741 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
     4801 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
     4861 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
     4921 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
     4981 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
     5041 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
     5101 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
     5161 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
     5221 agctagttga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
     5281 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
     5341 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
     5401 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
     5461 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
     5521 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
     5581 tagggcaaat tgccctagca ggggaaaaag gtcgaaaaga tctctttcc
//
