LOCUS       sequence-422673-       10795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic integration of genes (inserted in I-SceI-site) via 
            transposon Tn5 with SmR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10795)
  AUTHORS   Weihmann R, Kubicki S, Bitzenhofer NL, Domrose A, Bator I, Kirschen 
            LM, Kofler F, Funk A, Tiso T, Blank LM, Jaeger KE, Drepper T, Thies 
            S, Loeschcke A
  TITLE     The modular pYT vector series employed for chromosomal gene 
            integration and expression to produce carbazoles and glycolipids in 
            P. putida.
  JOURNAL   FEMS Microbes. 2022 Dec 19;4:xtac030. doi: 10.1093/femsmc/xtac030. 
            eCollection 2023.
  PUBMED    37333445
REFERENCE   2  (bases 1 to 10795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbes."; date: "2022-12-19"; volume: "4:xtac030. doi"; pages: " 
            10.1093/femsmc/xtac030. eCollection 2023"
COMMENT     Sequence Label: pYTRW11K_0S5
FEATURES             Location/Qualifiers
     source          1..10795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2130..2921
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    5096..5388
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      6036..6489
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(6517..6539)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6625..6765
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6680..6699
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6780..6797)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6951..7539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7031..7050)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7718..8512)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7829..7848)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8439..8458
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        8950..9165
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             9166..9969
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gactgactct tatacacaag
     1261 ttatctgcag cactcaacag atctccgtct acttgcgaca cctctcttaa ggtagctgga
     1321 ccggattcgg gtctagccta tatcgtcaat ccgttgatct atgtcgggtg cggagaaaga
     1381 ggtaatgaaa tgggcgatcg cccacagctt acacagacct aatcgtagag ttcctagtaa
     1441 tacttagtgg ctcgttacct cgttcttcca ggccaggggg ccgtacgcgc tcggcgaaca
     1501 gtataagcag attcgactca ctagggataa cagggtaatc tctgggtatc gactcaatct
     1561 cgcgctattg tgctatatcc gtggacggtt gcggtcaatt ctgtcggaaa atcaacggat
     1621 acgaacaatt actcgcgact tcctcaatac gtgctgcaac gcgcgcgtaa ctataacggt
     1681 cctaaggtag cgagcttact tgtgctgttg tgtaaggaca cgcttaaccc agagaattcc
     1741 agcgacctat tctacgacta cgcgtccatg gctcgaggag ctcggtaccg ccccactacc
     1801 cgatttatca agatttggcg gagagtcccc gcacaccgtg gaaacggatg aaggcacgaa
     1861 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     1921 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     1981 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     2041 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     2101 gcagtcgccc taaaacaaag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga
     2161 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     2221 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     2281 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     2341 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     2401 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     2461 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     2521 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     2581 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     2641 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     2701 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     2761 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     2821 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     2881 actacgtgaa aggcgagatc accaaggtag tcggcaaata agtcgactgg caaacagcta
     2941 ttatgggtat tatgggtgtg tatccagaac gacggatctt gacgcaattt ggcgtggctc
     3001 tcttaaggta gccctccgac caattgaggt gcgctctgac cgatcagttt agacttgtgt
     3061 ataagagtca gagcgtcctg aacggaacct ttcccgtttt ccaggatctg atcttccatg
     3121 tgacctccta acatggtaac gttcatgata acttctgctc ttcatcgtgc ggccgactgg
     3181 gctaaatctg tgttctcttc ggcggcgctg ggtgatcctc gccgtactgc ccgcttggtt
     3241 aacgtcgccg cccaattggc aaaatattct ggtaaatcaa taaccatctc atcagagggt
     3301 agtgaagcca tgcaggaagg cgcttaccga tttatccgca atcccaacgt ttctgccgag
     3361 gcgatcagaa aggctggcgc catgcaaaca gtcaagttgg ctcaggagtt tcccgaactg
     3421 ctggccattg aggacaccac ctctttgagt tatcgccacc aggtcgccga agagcttggc
     3481 aagctgggct ctattcagga taaatcccgc ggatggtggg ttcactccgt tctcttgctc
     3541 gaggccacca cattccgcac cgtaggatta ctgcatcagg agtggtggat gcgcccggat
     3601 gaccctgccg atgcggatga aaaggagagt ggcaaatggc tggcagcggc cgcaactagc
     3661 cggttacgca tgggcagcat gatgagcaac gtgattgcgg tctgtgaccg cgaagccgat
     3721 attcatgctt atctgcagga caaactggcg cataacgagc gcttcgtggt gcgctccaag
     3781 cacccacgca aggacgtaga gtctgggttg tatctgtacg accatctgaa gaaccaaccg
     3841 gagttgggtg gctatcagat cagcattccg caaaagggcg tggtggataa acgcggtaaa
     3901 cgtaaaaatc gaccagcccg caaggcgagc ttgagcctgc gcagtgggcg catcacgcta
     3961 aaacagggga atatcacgct caacgcggtg ctggccgagg agattaaccc gcccaagggt
     4021 gagaccccgt tgaaatggtt gttgctgacc agcgaaccgg tcgagtcgct agcccaagcc
     4081 ttgcgcgtca tcgacattta tacccatcgc tggcggatcg aggagttcca taaggcatgg
     4141 aaaaccggag caggagccga gaggcaacgc atggaggagc cggataatct ggagcggatg
     4201 gtctcgatcc tctcgtttgt tgcggtcagg ctgttacagc tcagagaaag cttcacgctg
     4261 ccgcaagcac tcagggcgca agggctgcta aaggaagcgg aacacgtaga aagccagtcc
     4321 gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa
     4381 cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat agctagactg
     4441 ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct ctgggaaggt
     4501 tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct gatggcgcag
     4561 gggatcaaga tctgacccgg gactagttgg tgtggaagtc ctaatatcgg tgatcaatat
     4621 agtggttgac atgctggcta gtcaacattg agccttttga tcatgcaaat atattacggt
     4681 attttacaat caaatatcaa acttaactat tgactttata acttatttag gtggtaacat
     4741 tcttataaaa aagaaaaaaa ttactgcaaa acagtactag cttttaactt gtatcctagg
     4801 ttatctatgc tgtctcacca tagagaatat tacctatttc agaatgtatg tccatgattc
     4861 gccgggtaaa tacatataat acacaaatct ggcttaataa agtctataat atatctcata
     4921 aagaagtgct aaattggcta gtgctatata tttttaagaa aatttctttt gactaagtcc
     4981 atatcgactt tgtaaaagtt cacattagca tacatatatt acacgagcca gaaatagtaa
     5041 cttttgccta aatcacaaat tgcaaaattt aattgcttgc aaaaggtcac atgcttataa
     5101 tcaacttttt taaaaattta aaatactttt ttatttttta tttttaaaca taaatgaaat
     5161 aatttattta ttgtttatga ttaccgaaac ataaaacctg ctcaagaaaa agaaactgtt
     5221 ttgtccttgg aaaaaaagca ctacctagga gcggccaaaa tgccgaggct ttcatagctt
     5281 aaactcttta cagaaaatag gcattataga tcagttcgag ttttcttatt cttccttccg
     5341 gttttatcgt cacagtttta cagtaaataa gtatcacctc ttagagttaa cctatactaa
     5401 aatttatgcc agttaactat gagataagca agtatcatct catttcatta cctgaagtcg
     5461 agtaaacaga aaatccaatt gttgatgaac ctcaatgact tagaactatc tatcggcaga
     5521 tcatataaag aggatttagg tactcgagaa cgctccgtac atgcagctaa acctccaagc
     5581 atctctaact tgggtagtgc tttctccacc attttctgtg cttgctcctt cgtggcaagt
     5641 ccagcccata atgcccagaa tgtagttgcg gattcgtatg acgttctgtg cttgattttt
     5701 gtgttgtagt caaagaaaag gtccggttaa acggatctcg cattgatgag gcaacgctaa
     5761 ttatcaacat atagattgtt atctatctgc atgaacacga aatctttact tgacgacttg
     5821 aggctgatgg tgtttatgca aagaaaccac tgtgtttaat atgtgtcact gtttgatatt
     5881 actgtcagcg tagaagataa tagtaaaagc ggttaataag tgtatttgag ataagtgtga
     5941 taaagttttt acagcgaaaa gacgataaat acaagaaaat gattacgagg atacggagag
     6001 aggtatgtac atgtgtattt atatactaag ctgccggcgg ttgtttgcaa gaccgagaaa
     6061 aggctagcaa gaatcgggtc attgtagcgt atgcgcctgt gaacattctc ttcaacaagt
     6121 ttgattccat tgcggtgaaa tggtaaaagt caaccccctg cgatgtatat tttcctgtac
     6181 aatcaatcaa aaagccaaat gatttagcat tatctttaca tcttgttatt ttacagattt
     6241 tatgtttaga tcttttatgc ttgcttttca aaaggcctgc aggcaagtgc acaaacaata
     6301 cttaaataaa tactactcag taataaccta tttcttagca tttttgacga aatttgctat
     6361 tttgttagag tcttttacac catttgtctc cacacctccg cttacatcaa caccaataac
     6421 gccatttaat ctaagcgcat caccaacatt ttctggcgtc agtccaccag ctaacataaa
     6481 atgttgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     6541 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     6601 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     6661 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     6721 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     6781 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     6841 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     6901 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     6961 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7021 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7081 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7141 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7201 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7261 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7321 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7381 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7441 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7501 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7561 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7621 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7681 agtatatatg agtaaacttg gtctgacagg agctcgctca gaagaactcg tcaagaaggc
     7741 gatagaaggc gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt
     7801 cagcccattc gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat
     7861 agcggtccgc cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca
     7921 ccatgatatt cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca
     7981 tccgcgcctt gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca
     8041 gatcatcctg atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt
     8101 tcgcttggtg gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat
     8161 cagccatgat ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg
     8221 gcacttcgcc caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg
     8281 cgcaaggaac gcccgtcgtg gccagccacg atagccgcgc tgcctcgtct tggagttcat
     8341 tcagggcacc ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc
     8401 ggaacacggc ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc
     8461 tctccaccca agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg
     8521 atcctcatcc tgtctcttga tcagatcttg atcccctgcg ccatcagatc cttggcggca
     8581 agaaagccat ccagtttact ttgcagggct tcccaacctt accagagggc gccccagctg
     8641 gcaattccgg ttcgcttgct gtccataaaa ccgcccagtc tagctatcgc catgtaagcc
     8701 cactgcaagc tacctgcttt ctctttgcgc ttgcgttttc ccttgtccag atagcccagt
     8761 agctgacatt catccgggcg atcgatggaa gccggcggca cctcgctaac ggattcacca
     8821 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt
     8881 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atcggggggg
     8941 ggggkkggkt tcaattcatc attttttttt tattcttttt tttgatttcg gtttccttga
     9001 aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga gcacagactt
     9061 agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc agtattctta
     9121 acccaactgc acagaacaaa aacctgcagg aaacgaagat aaatcatgtc gaaagctaca
     9181 tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt taatatcatg
     9241 cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa ggaattactg
     9301 gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt ggatatcttg
     9361 actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc caagtacaat
     9421 tttttactct tcgaagacag aaaatttgct gacattggta atacagtcaa attgcagtac
     9481 tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca cggtgtggtg
     9541 ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa ggaacctaga
     9601 ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg agaatatact
     9661 aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt tattgctcaa
     9721 agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc cggtgtgggt
     9781 ttagatgaca agggagacgc attgggtcaa cagtatagaa ccgtggatga tgtggtctct
     9841 acaggatctg acattattat tgttggaaga ggactatttg caaagggaag ggatgctaag
     9901 gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag atgcggccag
     9961 caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca aattagagct
    10021 tcaatttaat tatatcagtt attacccggg aatctcggtc gtaatgattt ttataatgac
    10081 gaaaaaaaaa aaattggaaa gaaaaccccc ccccccscmg cgttgggtcc tggccacggg
    10141 tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg
    10201 gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt
    10261 ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa
    10321 cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc
    10381 taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt
    10441 ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc
    10501 gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca
    10561 ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa
    10621 ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca
    10681 acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg
    10741 agctttaccg ctgggccatt ctcatgaaga atatcttgaa tttattgtca tatta
//
