LOCUS       sequence-422674-        6477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses TALED_Left-ND6-1397N in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Cho SI, Lim K, Hong S, Lee J, Kim A, Lim CJ, Ryou S, Lee JM, Mok YG,
            Chung E, Kim S, Han S, Cho SM, Kim J, Kim EK, Nam KH, Oh Y, Choi M, 
            An TH, Oh KJ, Lee S, Lee H, Kim JS
  TITLE     Engineering TALE-linked deaminases to facilitate precision adenine 
            base editing in mitochondrial DNA.
  JOURNAL   Cell. 2024 Jan 4;187(1):95-109.e26. doi: 10.1016/j.cell.2023.11.035.
  PUBMED    38181745
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.11"; journalName: "Cell"; date: "2024-01-4- 4";
            volume: "187"; issue: "1"; pages: "95-109.e26"
COMMENT     Sequence Label: TALED_Left-ND6-1397N
FEATURES             Location/Qualifiers
     source          1..6477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             978..1004
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1008..1034
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1038..1064
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(3651..3669)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3652..3669)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3689..3706)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3695..3919
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    4150..4271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4187..4206)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4241..4260
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4320..4336)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4320..4336)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4333..4355)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4344..4360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4368..4374,4375..4392,4393..4398))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4413..4434
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4551..4568)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4722..5310)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4802..4821)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5481..6272,6273..6341))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6104..6123
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6342..6446)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagatggct cttagtcgag
      901 ccgtctgtgg tacttcccga cagcttgctc cagtcttggg gtaccttggc tcccgacaaa
      961 agcactctct ccctgattac ccttacgatg tacctgacta cgccggctac ccttacgacg
     1021 taccagatta cgctggatat ccatacgatg tccctgatta tgctgggatc cgaattcaag
     1081 atctacgcac gctcggctac agccagcagc aacaggagaa gatcaaaccg aaggttcgtt
     1141 cgacagtggc gcagcaccac gaggcactgg tcggccatgg gtttacacac gcgcacatcg
     1201 ttgcgctcag ccaacacccg gcagcgttag ggaccgtcgc tgtcaagtat caggacatga
     1261 tcgcagcgtt gccagaggcg acacacgaag cgatcgttgg cgtcggcaaa cagtggtccg
     1321 gcgcacgcgc tctggaggcc ttgctcacgg tggcgggaga gttgagaggt ccaccgttac
     1381 agttggacac aggccaactt ctcaagattg caaaacgtgg cggcgtgacc gcagtggagg
     1441 cagtgcatgc atggcgcaat gcactgacgg gtgcccccct gaaccttaca cctgctcagg
     1501 tcgttgctat tgcatcacac gatgggggca aacaggcact cgagacagtg cagcggttgc
     1561 tgccagtgct gtgtcaagat cacggtttga ccccagagca agtggtggcc atcgcttcaa
     1621 acataggagg aaagcaagcc ctcgagactg ttcaaagact gcttcccgtt ctctgtcaag
     1681 cacatgggtt gactccagca caggttgttg ccattgctag taatattggt ggtaaacagg
     1741 cattggaaac tgtgcaaaga ctccttcctg tcctgtgtca ggaccatggg ttgaccccag
     1801 ctcaagtcgt cgctatcgct agtcatgatg gggggaaaca ggctctcgaa actgtgcaga
     1861 ggctgctccc cgtgctttgt caggctcacg gtttgacccc cgaccaggtc gttgcaatcg
     1921 cctctcatga cggcggcaag caagccctcg agactgtgca aaggctgctt cccgtcttgt
     1981 gccaagatca tggcctcact cctgatcagg tggtggccat tgcttcacac gatgggggca
     2041 agcaggctct tgaaaccgtt cagagacttt tgccagtcct gtgtcaggac catgggctca
     2101 caccagccca agtcgtcgca atcgcctccc acgacggcgg caagcaggca ttggaaaccg
     2161 tgcaaagact tttgccagtg ctgtgccaag cacacggcct caccccagca caagtggtgg
     2221 ctatcgctag caacggtggg ggcaagcagg cccttgagac agttcagagg ctccttcctg
     2281 ttctctgtca ggatcatggt ttgacaccag atcaggtggt cgctattgct tccaacaatg
     2341 gcgggaaaca agctttggag actgtgcaac ggttgctgcc tgttctctgc caagcacatg
     2401 gcttgacccc cgagcaggtg gtcgccatcg catccaatat tggtggaaaa caagctctgg
     2461 aaactgtcca gagacttttg ccagtgctct gtcaagctca tggcctcact cctgctcagg
     2521 ttgtggccat tgccagccac gatgggggta agcaagcact tgaaacagtt caaagactgc
     2581 ttcccgtgct ttgtcaggca cacgggctga ctcccgcaca agtcgtcgcc atcgcctcac
     2641 atgacggagg caaacaagca ctggagacag ttcaacgcct cctccctgtc ttgtgccaag
     2701 accacggact gactcctgca caggtggttg caatcgcaag ccatgatggc ggtaagcaag
     2761 ctttggagac agtccagcgc ttgcttcccg tgctctgtca agaccacggt ctgacccccg
     2821 ctcaagttgt ggctattgct tcccatgacg ggggcaagca ggctttggag actgtgcaaa
     2881 gattgcttcc tgtgttgtgc caggctcatg gactgacccc tgcccaggtg gtggccattg
     2941 cctcccacga tggaggtaag caggctctgg agacagttca aagattgctt ccagttcttt
     3001 gccaggatca cggtctgaca cctgatcagg tcgtggccat cgcatcaaat atagggggta
     3061 aacaagcttt ggagactgtc cagaggctcc tccccgtctt gtgtcaagcc cacggtctga
     3121 ctccggaaca ggtggtggcg attgcaagca acggcggcgg caaacaggct ctagagagca
     3181 ttgttgccca gctctccaga cctgatccgg cgctagccgc gttgacaaat gatcatcttg
     3241 ttgctcttgc ttgtcttggc ggacgacctg ccctggatgc tgtgaaaaag gggttggggg
     3301 gctctggatc aggtagctac gcacttggtc cttaccagat tagcgcaccc caactccccg
     3361 cctataatgg tcaaaccgtc gggacctttt actacgtaaa cgatgctggt gggctggaat
     3421 ccaaagtatt ctcctcaggg ggccctacac cctaccccaa ctacgccaat gctggtcatg
     3481 tagaagggca gtcagcactg tttatgcgcg ataatggtat aagcgagggg ttggtcttcc
     3541 ataacaaccc agagggtact tgtggcttct gtgtgaatat gactgaaacc cttctgcccg
     3601 aaaatgccaa gatgactgtc gtcccacctg aaggctagtc agggccctat tctatagtgt
     3661 cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     3721 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     3781 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     3841 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     3901 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat
     3961 ccccacgctg cagtgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     4021 ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     4081 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     4141 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4201 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4261 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     4321 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4381 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     4441 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     4501 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     4561 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4621 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4681 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4741 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4801 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     4861 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     4921 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     4981 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5041 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5101 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5161 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5221 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5281 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5341 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5401 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     5461 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     5521 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     5581 gagggcttac catctggccc cagtgctgca atgataccgc gagatccacg ctcaccggct
     5641 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     5701 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     5761 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     5821 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5881 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5941 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6001 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6061 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6121 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6181 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6241 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6301 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     6361 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6421 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc
//
