LOCUS       sequence-422678-       15453 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic integration of genes (inserted in I-SceI-site) via 
            transposon Tn7 with GmR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15453)
  AUTHORS   Tiso T, Ihling N, Kubicki S, Biselli A, Schonhoff A, Bator I, Thies 
            S, Karmainski T, Kruth S, Willenbrink AL, Loeschcke A, Zapp P, Jupke
            A, Jaeger KE, Buchs J, Blank LM
  TITLE     Integration of Genetic and Process Engineering for Optimized 
            Rhamnolipid Production Using Pseudomonas putida.
  JOURNAL   Front Bioeng Biotechnol. 2020 Aug 20;8:976. doi: 
            10.3389/fbioe.2020.00976. eCollection 2020.
  PUBMED    32974309
REFERENCE   2  (bases 1 to 15453)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2020-08-20"; volume: "8:976. doi"; 
            pages: " 10.3389/fbioe.2020.00976. eCollection 2020"
COMMENT     Sequence Label: pYTSK01K_0G7
FEATURES             Location/Qualifiers
     source          1..15453
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  1204..1369
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     promoter        join(1939..1944,1945..1961,1962..1967)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2156..2689
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     mobile_element  8840..9064
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     misc_feature    9753..10045
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      10693..11146
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(11174..11196)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11282..11422
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11337..11356
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11437..11454)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11608..12196)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11688..11707)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12375..13169)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12486..12505)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     13096..13115
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        13607..13822
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             13823..14626
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaatgtgggc ggacaaaata gttgggaact gggaggggtg gaaatggagt ttttaaggat
     1261 tatttaggga agagtgacaa aatagatggg aactgggtgt agcgtcgtaa gctaatacga
     1321 aaattaaaaa tgacaaaata gtttggaact agatttcact tatctggttt atctgcagca
     1381 ctcaacagat ctccgtctac ttgcgacacc tctcttaagg tagctggacc ggattcgggt
     1441 ctagcctata tcgtcaatcc gttgatctat gtcgggtgcg gagaaagagg taatgaaatg
     1501 ggcgatcgcc cacagcttac acagacctaa tcgtagagtt cctagtaata cttagtggct
     1561 cgttacctcg ttcttccagg ccagggggcc gtacgcgctc ggcgaacagt ataagcagat
     1621 tcgactcact agggataaca gggtaatctc tgggtatcga ctcaatctcg cgctattgtg
     1681 ctatatccgt ggacggttgc ggtcaattct gtcggaaaat caacggatac gaacaattac
     1741 tcgcgacttc ctcaatacgt gctgcaacgc gcgcgtaact ataacggtcc taaggtagcg
     1801 agcttacttg tgctgttgtg taaggacacg cttaacccag agaattccag cgacctattc
     1861 tacgactacg cgtccatggc tcgaggagct cggtaccgcc ccactacccg atttatcaag
     1921 atttggcgga gagtcccatt gacataagcc tgttcggttc gtaaactgta atgcaagtag
     1981 cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac gcagcggtgg taacggcgca
     2041 gtggcggttt tcatggcttg ttatgactgt ttttttgtac agtctatgcc tcgggcatcc
     2101 aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt
     2161 acgcagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taggtggctc
     2221 aagtatgggc atcattcgca catgtaggct cggccctgac caagtcaaat ccatgcgggc
     2281 tgctcttgat cttttcggtc gtgagttcgg agacgtagcc acctactccc aacatcagcc
     2341 ggactccgat tacctcggga acttgctccg tagtaagaca ttcatcgcgc ttgctgcctt
     2401 cgaccaagaa gcggttgttg gcgctctcgc ggcttacgtt ctgcccaagt ttgagcagcc
     2461 gcgtagtgag atctatatct atgatctcgc agtctccggc gagcaccgga ggcagggcat
     2521 tgccaccgcg ctcatcaatc tcctcaagca tgaggccaac gcgcttggtg cttatgtgat
     2581 ctacgtgcaa gcagattacg gtgacgatcc cgcagtggct ctctatacaa agttgggcat
     2641 acgggaagaa gtgatgcact ttgatatcga cccaagtacc gccacctaag tcgactggca
     2701 aacagctatt atgggtatta tgggtgtgta tccagaacga cggatcttga cgcaatttgg
     2761 cgtggctctc ttaaggtagc cctccgacca attgaggtgc gctctgaccg atcagtttag
     2821 tcactcttcc ccataaacca tttccaagaa tctttgtgct tcctcagtta tccgctcttt
     2881 agataacgtt gcacttctta ataatcgcca gcgcctaagc tcaacatctt cctgtttaag
     2941 cttaataaaa gcttggctaa tccgtctaat ttgataatct tccacactct ctgagtaacg
     3001 ctttaagcaa agcgctacca aaggcagttt ctgtagattt tttgcaagag aggttccgtt
     3061 aggagtttgc tttaacagcc agctcgatgt cgctcttggg tgatcaaggc tactatctag
     3121 acgctttatg attcttaata gttgtcgtac agcaattcga tctctttggt tccaatcaac
     3181 tctaggggcg ggtgccagac gctcttgttg atgctgttga ttccagtgaa ctagccaatc
     3241 cctgtcatgt cggtaaagcc aagcgtatag caccccaccc tctaaagact gtcttgccgc
     3301 cttaattcct tggtatttat gcactagttg ctgccagtct ttatgcttaa cagataaatc
     3361 ttcagagtta ggttgcacag actggctaac aggtcttgtc gttatagagt gctcagtaag
     3421 agcacttgcc tgctgtagcg cttctataac cgttagtttt ggcaataagg cttgccacac
     3481 aatactatgc tgtaaataac taaaggcttt tctatgctta cggaatatac ttttcagcca
     3541 acacgtgtcc ttgttctctg ccaactttaa atccagtttc tctagtgcct catcactaaa
     3601 agtctgcctc actctctccg ccaccaagtc atgacgaatg tgcttgcttt tggttagccc
     3661 tagatcctgc gctaagcgct gataaaacag cgtccactgc tcaaggcttg gggaaagctc
     3721 ttgcgctcgt ggagcatcta acagaggggc tatataagca gctagtgctg ttaattgaga
     3781 tagggagtct ttggggtagt ctgaaagcag ctcagtatga cccaaagccc aaaattgatg
     3841 tcggtgatca tctacagctc tatcaaagaa gactaaagca ccgtgttttg gacaatatgg
     3901 caaagcgggc aaataccaat ctcgttgcca aaaggcttcc ccatacctat ttagctgaag
     3961 agcaacgcaa tcagggcagt atctaaagcg gttatcgctc ttaactctag aagcagcgac
     4021 tcctagcatt aaatgcaccg caccttgcgc ttggtactcc attaaccgaa tagcttcgtc
     4081 tcggcgctcc ttgcctacaa acggagcata taaagggaat aaggtatgct cataaataag
     4141 ctgctgaaca gcgtaacgtc ctgtttgatg tagatgtctt gctatcacac ctaaatgcga
     4201 gggcagacct aaggtagcga ccaccttgcg gttgccatac acctcatcca acagctgctt
     4261 aggactaaca atcccttgat aaacgcctgc ccgtgcaata gtgctataaa tcagctcatt
     4321 cgagtacgga acaggaaagt ttctcatggc taacctgctt gtttaaataa gctcgccata
     4381 tccactatta cccctttccc ttttaaccgt tcatgcatgg ttttttcagg ttggcgttgt
     4441 gaatagatat aacgtaaatc cgtatcaggc aagctatccc agtcgctggg cttgactacc
     4501 tttatagccg aaaccttttt actcttggag ggcttttcta gttctgacac taccgtttcg
     4561 ccttccatca accactgcaa aacaagaggc agtaactttt gtcttgtcat cgttggattc
     4621 tggctaaacg cttttttaat agtgggaatt aacaggcttg aatcgtaatc ctctttcagc
     4681 atcagatata aatgacgctg atcttcggta tctaactctt gaagggcttt ttcttctggt
     4741 gtttgttctt gtatcgctgc gatatctagc tgaagttgga ttaaccgttt atcaatctcg
     4801 ggaacgacta gatcagaata acgagcaatg cgttctggga tacccgagcg taatgcctct
     4861 agcatggggt gcacaggctt taactcatct tgatacactt gccgcaataa accagcggta
     4921 atacgctcat tgcctaaagc tagcgcacgg agctgagcga gtacaaaaag ttttactaca
     4981 atgtccatca ctccttggct tagctcatac cacacatcac ggacctcatc cgataacagc
     5041 gcatctttgc gttgtaaaag ctgtaattgc caaagattat ccgtaaaagc gatccactct
     5101 tgattgggct ttccacgttg cgtttgttgt ataggatccc agaatatagc tccaaaccct
     5161 gcccctctac gtgcagaccg caaatcagcc tcaaaaatct ctcgtgcttt aggggtacca
     5221 atcaacatca ctggtacgcc aataatattc accatcgtca caaaaaagtt cagcatctct
     5281 tgagatccac ccgaacgaga gcggcttaaa tgctgaattt catcaataac caacaaccct
     5341 aaagcatgtg cattggctat ttgcgacatc aaagccaaca tggtttctat accatgacgt
     5401 tttaagccat aacgacgctc atagttcgag cccaaggctc gatccaacgc tctgaaaaaa
     5461 ttcaagcaga tttcttttag cgaaccatta tgcgagcagt ctattttcaa atacaccacc
     5521 tgctctacat tgagttcacg atggtaaatc acctgaggat acgtggctag aatacgatga
     5581 agagaggtcg tcttcccact accagaacaa ccaattaaca ataagctttg tgccgtagat
     5641 cgtgcctcct caaagcgaaa tgtctccaac tctcccgttt gaacacgctc ataaccattt
     5701 tgtaaatgct tttgtaaatc tcctgtttta ggatttctgc ctacgtagcc acctcgaatc
     5761 atgaccgaaa tacgctcact tagtagcaaa tgcgtaccta atggctgaaa atagtcatct
     5821 ggaatacgac aaatggtatg agctctgata acacgggact tttgcaagtc agaggaagta
     5881 agctgtaaag aggatttcag tgatgcagca ctattcactg actcttgtaa tggtggtaag
     5941 gcctcgataa aaggattatc acgataagcc tctacccccg tatcacgata aactgcttga
     6001 atccgggtag cactcatgac tcatcctttt ctggtggatc ctgaaataat tcaggcacgt
     6061 atgtgggtag gctgtaatct tcttgatcat ccgcttccac tgcgttgaaa ggaataactt
     6121 tagcctcatc acctgaagag cttggcttca aatgctccgc acgtttttta cgctccgagg
     6181 tcacggcttc ttttttatta gttttaatct gcttaatgcg tgttgatttg ggctcagtag
     6241 tactgggcgt taacttattc gctttctgaa tggtttgctg aataaacgcc tcaagctccc
     6301 tgcgtttagt taactcatcc tgcttcgcat tggctttatt gtgtttttct tgtgcttgta
     6361 tatcccaaac ctcccaaaat gagagacctt taaactgccg actacgttcc gtcagattac
     6421 agcgccaaaa tacacggctg ccaacttgcg gaaacaaata aatcgtatca accagcactg
     6481 ggtcataagc cgcttctaaa tgttgaggtc tagctatatc agtgctccgc tgcaaccaac
     6541 cctcacgcag aatctctgac cccgagtaat acaaacccca caaattaacg ccaaatgaag
     6601 aaatagagac ctttcggcga ggcagtaacg ctactcgcaa ctgctcttgc tccacagccc
     6661 ttaaactacc tgtacgatgc tgcatacccc attgccatag ctggacagga atagacggta
     6721 aatctgtagg aaaatcagca tctcgatcgt atttatccat caccagatgg ttatttctga
     6781 ataagatcgt acgcaaaata atttgtgtga actcaaatac cgacagagat gcatctaacc
     6841 tatagtctgt ttcaccatgg cttttgatcc gactgccctc tacaatgcca ggtgcaaagg
     6901 acttaaactc ggcttgtagt gttctaaaag tgctttccac tatgccttta gcatcgccac
     6961 gtctaggtgg agcactttcc actcgcacat taaaactaga aactaaggct tcgacctgat
     7021 gactcattaa ttcgccacgg tccgctagca acacatctgg caaacctaca cacggccagt
     7081 ctgagctact aatctcaata tcatgctggg cacaaatggc cgttttgtca gagcaagcat
     7141 ttacaaaagc ctgcatcgcc accacataag acggattttc aaagccgata taaaagcccg
     7201 tgatcatccg actaaacaca tcaatcacaa tgtaaagcgt tggtcttcct atgatttttt
     7261 ggcgatcatg atgatccact aaataaatat cagcaatcgt ggcatcaatc tcataacgac
     7321 tcccagggcc taacgcctga gaagtggctg tactacttaa gggtcgtacg tcttttttat
     7381 atacccctgc tttaactcta gactttaagc gctgagcttt agggtattct cgatcataaa
     7441 aataacgaaa ctgacgtagt gttgggtaat cctcttgggg aatgcgagga aaatactgag
     7501 caaacaagtc cacaaatcgt ctataggcaa cggtggtctt tgtacctttt tgatttaaca
     7561 ggtgcttttc tatggtcaac ctaaaaaggc gttcaatctc gggcgttacc ttggttcctt
     7621 cacccttacc atattctctg gctcggccaa tctttgctgt tcctgtcgct gaacgtcttt
     7681 cccctggtgc accgctgttt ttgtagtcag gaattaatgc attaggcgtt tgaccacgct
     7741 gccagtaacg gcgtaacaac ttataaactg tagccttagt aaccttatgt tcttggacca
     7801 catgctcaac gagttcgctt ctgactttag ggtcgaaacg atccttacta ttaataatag
     7861 gaagaatttt tcgataatcc tcctcgcgct tctggaagct gacagaatct acagacggct
     7921 cttctaaatc aagatgtaca taagggtcat cagttctaac taagcggcct tcatctaaat
     7981 actgcattag caacaactca gccctagctt gtggaactcc tttatcagcg cttatttgca
     8041 tccagacaac ttggccatcc tctatagcca aaatgcgata cggatcatta tcaaatagca
     8101 caacctcatt aatttgccac atagcgcaac tcctccatat tcactacttg gctaatacag
     8161 agatctgcac acttatttgc cctgaaagac ttataaatat tgaacttaat aaaaccattt
     8221 gcggttaagg ctcgtatctc actcaatgtt ttgcctaact ccaaatcata agcaatatca
     8281 acctgcttac agacattgat aatgttttca tctccttttt cttgcaggat atgggccaat
     8341 ggggatagtt gtgctaaaag ctccgcagaa acttcttctg ttttcactga ataaagccat
     8401 tcaatatttt cttttactac gggatttatt tctttatcag taaaaatgaa ccaaggaatt
     8461 tgcttttgct gccaatagcg acgctctagt tctagttttt ctaaggtacg ctcgtcttgt
     8521 aaggctgctg caggtttgac ttgaatagca aactgctcaa aaggaccatc tttgcagtcc
     8581 actaaaaaat cagtagacat aacctgatct acaccacgaa taacaggatg cttaatacca
     8641 ctatctattg caatctgcct ggtatcacta ggtaataagg ggaactgctc gcgtatatct
     8701 agcacgctgc tctcccactc aagactgaga aaaacagcaa gctctaagtc agatagcaaa
     8761 tgatggactc gtcccgtctt atgagaataa atacggtggg aacgacctga agaaggaact
     8821 tcttgtactg ttagccatgg aatatagtct ttaccatgcc cttggccacg cccctcttta
     8881 atacgacggg caatttgcac ttcagaaaat gaagagtttg ctttagccat aacaaaagtc
     8941 cagtatgctt tttcacagca taactggact gatttcagtt tacaactatt ctgtctagtt
     9001 taagacttta ttgtcatagt ttagatctat tttgttcagt ttaagacttt attgtccgcc
     9061 cacacccgct tacgcagggc atccatttat tactcaaccg taaccgattt tgccaggtta
     9121 cgcggctggt caacgtcggt gcctttgatc agcgcgacat ggtaagccag cagctgcagc
     9181 ggaacggtgt agaagatcgg tgcaatcacc tcttccacat gcggcatctc gacccgggac
     9241 tagttggtgt ggaagtccta atatcggtga tcaatatagt ggttgacatg ctggctagtc
     9301 aacattgagc cttttgatca tgcaaatata ttacggtatt ttacaatcaa atatcaaact
     9361 taactattga ctttataact tatttaggtg gtaacattct tataaaaaag aaaaaaatta
     9421 ctgcaaaaca gtactagctt ttaacttgta tcctaggtta tctatgctgt ctcaccatag
     9481 agaatattac ctatttcaga atgtatgtcc atgattcgcc gggtaaatac atataataca
     9541 caaatctggc ttaataaagt ctataatata tctcataaag aagtgctaaa ttggctagtg
     9601 ctatatattt ttaagaaaat ttcttttgac taagtccata tcgactttgt aaaagttcac
     9661 attagcatac atatattaca cgagccagaa atagtaactt ttgcctaaat cacaaattgc
     9721 aaaatttaat tgcttgcaaa aggtcacatg cttataatca acttttttaa aaatttaaaa
     9781 tactttttta ttttttattt ttaaacataa atgaaataat ttatttattg tttatgatta
     9841 ccgaaacata aaacctgctc aagaaaaaga aactgttttg tccttggaaa aaaagcacta
     9901 cctaggagcg gccaaaatgc cgaggctttc atagcttaaa ctctttacag aaaataggca
     9961 ttatagatca gttcgagttt tcttattctt ccttccggtt ttatcgtcac agttttacag
    10021 taaataagta tcacctctta gagttaacct atactaaaat ttatgccagt taactatgag
    10081 ataagcaagt atcatctcat ttcattacct gaagtcgagt aaacagaaaa tccaattgtt
    10141 gatgaacctc aatgacttag aactatctat cggcagatca tataaagagg atttaggtac
    10201 tcgagaacgc tccgtacatg cagctaaacc tccaagcatc tctaacttgg gtagtgcttt
    10261 ctccaccatt ttctgtgctt gctccttcgt ggcaagtcca gcccataatg cccagaatgt
    10321 agttgcggat tcgtatgacg ttctgtgctt gatttttgtg ttgtagtcaa agaaaaggtc
    10381 cggttaaacg gatctcgcat tgatgaggca acgctaatta tcaacatata gattgttatc
    10441 tatctgcatg aacacgaaat ctttacttga cgacttgagg ctgatggtgt ttatgcaaag
    10501 aaaccactgt gtttaatatg tgtcactgtt tgatattact gtcagcgtag aagataatag
    10561 taaaagcggt taataagtgt atttgagata agtgtgataa agtttttaca gcgaaaagac
    10621 gataaataca agaaaatgat tacgaggata cggagagagg tatgtacatg tgtatttata
    10681 tactaagctg ccggcggttg tttgcaagac cgagaaaagg ctagcaagaa tcgggtcatt
    10741 gtagcgtatg cgcctgtgaa cattctcttc aacaagtttg attccattgc ggtgaaatgg
    10801 taaaagtcaa ccccctgcga tgtatatttt cctgtacaat caatcaaaaa gccaaatgat
    10861 ttagcattat ctttacatct tgttatttta cagattttat gtttagatct tttatgcttg
    10921 cttttcaaaa ggcctgcagg caagtgcaca aacaatactt aaataaatac tactcagtaa
    10981 taacctattt cttagcattt ttgacgaaat ttgctatttt gttagagtct tttacaccat
    11041 ttgtctccac acctccgctt acatcaacac caataacgcc atttaatcta agcgcatcac
    11101 caacattttc tggcgtcagt ccaccagcta acataaaatg ttgcctcgcg cgtttcggtg
    11161 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
    11221 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
    11281 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
    11341 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
    11401 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    11461 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    11521 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    11581 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    11641 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    11701 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    11761 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    11821 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    11881 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    11941 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    12001 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    12061 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    12121 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    12181 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    12241 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    12301 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    12361 tgacaggagc tcgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
    12421 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
    12481 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
    12541 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
    12601 tcgccatggg tcacgacgag atcctcgccg tcgggcatcc gcgccttgag cctggcgaac
    12661 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
    12721 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
    12781 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
    12841 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
    12901 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
    12961 agccacgata gccgcgctgc ctcgtcttgg agttcattca gggcaccgga caggtcggtc
    13021 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
    13081 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
    13141 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
    13201 gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg
    13261 cagggcttcc caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc
    13321 cataaaaccg cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc
    13381 tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat ccgggcgatc
    13441 gatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa
    13501 ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc
    13561 gccatctcca gcagccgcac gcggcgcatc gggggggggg gkksrkttca attcatcatt
    13621 ttttttttat tctttttttt gatttcggtt tccttgaaat ttttttgatt cggtaatctc
    13681 cgaacagaag gaagaacgaa ggaaggagca cagacttaga ttggtatata tacgcatatg
    13741 tagtgttgaa gaaacatgaa attgcccagt attcttaacc caactgcaca gaacaaaaac
    13801 ctgcaggaaa cgaagataaa tcatgtcgaa agctacatat aaggaacgtg ctgctactca
    13861 tcctagtcct gttgctgcca agctatttaa tatcatgcac gaaaagcaaa caaacttgtg
    13921 tgcttcattg gatgttcgta ccaccaagga attactggag ttagttgaag cattaggtcc
    13981 caaaatttgt ttactaaaaa cacatgtgga tatcttgact gatttttcca tggagggcac
    14041 agttaagccg ctaaaggcat tatccgccaa gtacaatttt ttactcttcg aagacagaaa
    14101 atttgctgac attggtaata cagtcaaatt gcagtactct gcgggtgtat acagaatagc
    14161 agaatgggca gacattacga atgcacacgg tgtggtgggc ccaggtattg ttagcggttt
    14221 gaagcaggcg gcagaagaag taacaaagga acctagaggc cttttgatgt tagcagaatt
    14281 gtcatgcaag ggctccctat ctactggaga atatactaag ggtactgttg acattgcgaa
    14341 gagcgacaaa gattttgtta tcggctttat tgctcaaaga gacatgggtg gaagagatga
    14401 aggttacgat tggttgatta tgacacccgg tgtgggttta gatgacaagg gagacgcatt
    14461 gggtcaacag tatagaaccg tggatgatgt ggtctctaca ggatctgaca ttattattgt
    14521 tggaagagga ctatttgcaa agggaaggga tgctaaggta gagggtgaac gttacagaaa
    14581 agcaggctgg gaagcatatt tgagaagatg cggccagcaa aactaaaaaa ctgtattata
    14641 agtaaatgca tgtatactaa actcacaaat tagagcttca atttaattat atcagttatt
    14701 acccgggaat ctcggtcgta atgattttta taatgacgaa aaaaaaaaaa ttggaaagaa
    14761 aacccccccc ccccgcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc
    14821 gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat
    14881 acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg
    14941 aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac
    15001 cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc
    15061 tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca
    15121 taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac
    15181 ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc
    15241 cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt
    15301 tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca
    15361 ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgct gggccattct
    15421 catgaagaat atcttgaatt tattgtcata tta
//
