LOCUS       sequence-422682-       13056 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic integration of genes (inserted in I-SceI-site) via 
            rrn-interposon/SacB with TcR and eYFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13056)
  AUTHORS   Weihmann R, Kubicki S, Bitzenhofer NL, Domrose A, Bator I, Kirschen 
            LM, Kofler F, Funk A, Tiso T, Blank LM, Jaeger KE, Drepper T, Thies 
            S, Loeschcke A
  TITLE     The modular pYT vector series employed for chromosomal gene 
            integration and expression to produce carbazoles and glycolipids in 
            P. putida.
  JOURNAL   FEMS Microbes. 2022 Dec 19;4:xtac030. doi: 10.1093/femsmc/xtac030. 
            eCollection 2023.
  PUBMED    37333445
REFERENCE   2  (bases 1 to 13056)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbes."; date: "2022-12-19"; volume: "4:xtac030. doi"; pages: " 
            10.1093/femsmc/xtac030. eCollection 2023"
COMMENT     Sequence Label: pYTRW26K_1Ti1
FEATURES             Location/Qualifiers
     source          1..13056
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(2170..2172,2173..2175,2176..2889)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2215..2236)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2476..2495)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2823..2844
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        join(3066..3071,3072..3088,3089..3094)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             3142..4332
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(3494..3513)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    5472..5764
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      6412..6865
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(6893..6915)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7001..7141
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7056..7075
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7156..7173)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7327..7915)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7407..7426)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8094..8888)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8205..8224)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8815..8834
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        9327..9542
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             9543..10346
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        11188..11633
                     /gene="sacR"
                     /label=sacB promoter
                     /note="sacB promoter and control region"
     CDS             join(11634..11720,11721..13055)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVSEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gagacgccac gcgatcgaac
     1261 gtcgttaact ctggcaggca attaaaggga acgtgtatct gacgcaaaga aaactggaaa
     1321 ttggcgagag aatcttctct ctgtctatcg aagactgacc acgcggaggc gtgcgtcttg
     1381 ttagcgtgcg gggtactctt gctatccatt tgagtcacac gacactcgct gttctcgact
     1441 ttacacttgt aagcgacggt attgaacgac gcttctgccc accatcgtta caaccagact
     1501 gatactagat ctatactgtc agccattcag acgaaaccag ccggagatta ccgagcactc
     1561 tattacgtcg gcgaccacta ctgagctagt ggagccgtgg gtaacgatca taccactaag
     1621 aacctctctc gacgcaagcg attacactgc tgtcacatca taatcgtctt gctattcacg
     1681 agttgaccag acactggata gcttgtcact tgaagtattc tgcacgacag ggtgcgtgta
     1741 cctatctgca gcactcaaca gatctccgtc tacttgcgac acctctctta aggtagctgg
     1801 accggattcg ggtctagcct atatcgtcaa tccgttgatc tatgtcgggt gcggagaaag
     1861 aggtaatgaa atgggcgatc gcccacagct tacacagacc taatcgtaga gttcctagta
     1921 atacttagtg gctcgttacc tcgttcttcc aggccagggg gccgtacgcg ctcggcgaac
     1981 agtataagca gattcgactc actagggata acagggtaat ctctgggtat cgactcaatc
     2041 tcgcgctatt gtgctatatc cgtggacggt tgcggtcaat tctgtcggaa aatcaacgga
     2101 tacgaacaat tactcgcgac ttcctcaata cgtgctgcaa cgcgcgcgaa ctttagaagg
     2161 agatatacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2221 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2281 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2341 tggcccaccc tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac
     2401 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2461 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2521 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2581 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     2641 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     2701 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2761 gacaaccact acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     2821 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     2881 tacaagtaac gcgcgcgtaa ctataacggt cctaaggtag cgagcttact tgtgctgttg
     2941 tgtaaggaca cgcttaaccc agagaattcc agcgacctat tctacgacta cgcgtccatg
     3001 gctcgaggag ctcggtaccg ccccactacc cgatttatca agatttggcg gagagtccct
     3061 catgtttgac agcttatcat cgataagctt taatgcggta gtttatcaca gttaaattgc
     3121 taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat cctcggcacc
     3181 gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg cctcttgcgg
     3241 gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc gctatatgcg
     3301 ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt tggccgccgc
     3361 ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat ggcgaccaca
     3421 cccgtcctgt ggatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     3481 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     3541 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     3601 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     3661 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg accgatgccc
     3721 ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc
     3781 gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg
     3841 gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg
     3901 gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt
     3961 ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg
     4021 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc
     4081 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag
     4141 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat tggaccgctg
     4201 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta
     4261 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc
     4321 acctcgacct gagtcgactg gcaaacagct attatgggta ttatgggtgt gtatccagaa
     4381 cgacggatct tgacgcaatt tggcgtggct ctcttaaggt agccctccga ccaattgagg
     4441 tgcgctctga ccgatcagtt tagggtgagc caacccggcc gatccggaga gacgggcctc
     4501 aaagccgcgt gacgacggct gtgggcccgt aacaaaatcc ccgcaataag ctcccgtgag
     4561 cgtcggttga acagccctgg tcggccccat cagtagcccg aatacttcgc tttacgggtc
     4621 ctgggccggg gtgcgatacc ttgcagaaat cgaggccgtt cgttaattcc tgttgcattc
     4681 gtaccgccta tatttgtctc tttgccggct tatcatcaca agcatagcat agccatttat
     4741 cggagcgcct ccgtacacgg tatgatcgga cgcctcgtga gatcaatacg tataccaggt
     4801 gtcctgtgag cagcgaaagc ctatacgcga gatacactgc caaaaatccg cgtgattacg
     4861 agtcgtggca aatttggtct ggctgtggtc tagacattcc aggcggtgcg tctgctctcg
     4921 ggtgcctcta gttgctggct agatagacta gccgctggta aactggtgtg gaagtccata
     4981 tatcggtgat caatatagtg gttgacatgc tggctagtca acattgagcc ttttgatcat
     5041 gcaaatatat tacggtattt tacaatcaaa tatcaaactt aactattgac tttataactt
     5101 atttaggtgg taacattctt ataaaaaaga aaaaaattac tgcaaaacag tactagcttt
     5161 taacttgtat cctaggttat ctatgctgtc tcaccataga gaatattacc tatttcagaa
     5221 tgtatgtcca tgattcgccg ggtaaataca tataatacac aaatctggct taataaagtc
     5281 tataatatat ctcataaaga agtgctaaat tggctagtgc tatatatttt taagaaaatt
     5341 tcttttgact aagtccatat cgactttgta aaagttcaca ttagcataca tatattacac
     5401 gagccagaaa tagtaacttt tgcctaaatc acaaattgca aaatttaatt gcttgcaaaa
     5461 ggtcacatgc ttataatcaa cttttttaaa aatttaaaat acttttttat tttttatttt
     5521 taaacataaa tgaaataatt tatttattgt ttatgattac cgaaacataa aacctgctca
     5581 agaaaaagaa actgttttgt ccttggaaaa aaagcactac ctaggagcgg ccaaaatgcc
     5641 gaggctttca tagcttaaac tctttacaga aaataggcat tatagatcag ttcgagtttt
     5701 cttattcttc cttccggttt tatcgtcaca gttttacagt aaataagtat cacctcttag
     5761 agttaaccta tactaaaatt tatgccagtt aactatgaga taagcaagta tcatctcatt
     5821 tcattacctg aagtcgagta aacagaaaat ccaattgttg atgaacctca atgacttaga
     5881 actatctatc ggcagatcat ataaagagga tttaggtact cgagaacgct ccgtacatgc
     5941 agctaaactt ccaagcatct ctaacttggg tagtgctttc tccaccattt tctgtgcttg
     6001 ctccttcgtg gcaagtccag cccataatgc ccagaatgta gttgcggatt cgtatgacgt
     6061 tctgtgcttg atttttgtgt tgtagtcaaa gaaaaggtcc ggttaaacgg atctcgcatt
     6121 gatgaggcaa cgctaattat caacatatag attgttatct atctgcatga acacgaaatc
     6181 tttacttgac gacttgaggc tgatggtgtt tatgcaaaga aaccactgtg tttaatatgt
     6241 gtcactgttt gatattactg tcagcgtaga agataatagt aaaagcggtt aataagtgta
     6301 tttgagataa gtgtgataaa gtttttacag cgaaaagacg ataaatacaa gaaaatgatt
     6361 acgaggatac ggagagaggt atgtacatgt gtatttatat actaagctgc cggcggttgt
     6421 ttgcaagacc gagaaaaggc tagcaagaat cgggtcattg tagcgtatgc gcctgtgaac
     6481 attctcttca acaagtttga ttccattgcg gtgaaatggt aaaagtcaac cccctgcgat
     6541 gtatattttc ctgtacaatc aatcaaaaag ccaaatgatt tagcattatc tttacatctt
     6601 gttattttac agattttatg tttagatctt ttatgcttgc ttttcaaaag gcctgcaggc
     6661 aagtgcacaa acaatactta aataaatact actcagtaat aacctatttc ttagcatttt
     6721 tgacgaaatt tgctattttg ttagagtctt ttacaccatt tgtctccaca cctccgctta
     6781 catcaacacc aataacgcca tttaatctaa gcgcatcacc aacattttct ggcgtcagtc
     6841 caccagctaa cataaaatgt tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
     6901 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     6961 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
     7021 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
     7081 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     7141 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7201 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7261 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7321 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7381 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7441 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7501 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7561 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7621 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7681 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7741 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     7801 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7861 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7921 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7981 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8041 ttttaaatca atctaaagta tatatgagta aacttggtct gacaggagct cgctcagaag
     8101 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     8161 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     8221 aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa
     8281 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     8341 tcctcgccgt cgggcatccg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     8401 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     8461 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     8521 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     8581 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     8641 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     8701 tcgtcttgga gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     8761 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     8821 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     8881 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc cctgcgccat
     8941 cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc aaccttacca
     9001 gagggcgccc cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc ccagtctagc
     9061 tatcgccatg taagcccact gcaagctacc tgctttctct ttgcgcttgc gttttccctt
     9121 gtccagatag cccagtagct gacattcatc cgggcgatcg atggaagccg gcggcacctc
     9181 gctaacggat tcaccactcc aagaattgga gccaatcaat tcttgcggag aactgtgaat
     9241 gcgcaaacca acccttggca gaacatatcc atcgcgtccg ccatctccag cagccgcacg
     9301 cggcgcatcg gggggggggg gggggtttca attcatcatt ttttttttat tctttttttt
     9361 gatttcggtt tccttgaaat ttttttgatt cggtaatctc cgaacagaag gaagaacgaa
     9421 ggaaggagca cagacttaga ttggtatata tacgcatatg tagtgttgaa gaaacatgaa
     9481 attgcccagt attcttaacc caactgcaca gaacaaaaac ctgcaggaaa cgaagataaa
     9541 tcatgtcgaa agctacatat aaggaacgtg ctgctactca tcctagtcct gttgctgcca
     9601 agctatttaa tatcatgcac gaaaagcaaa caaacttgtg tgcttcattg gatgttcgta
     9661 ccaccaagga attactggag ttagttgaag cattaggtcc caaaatttgt ttactaaaaa
     9721 cacatgtgga tatcttgact gatttttcca tggagggcac agttaagccg ctaaaggcat
     9781 tatccgccaa gtacaatttt ttactcttcg aagacagaaa atttgctgac attggtaata
     9841 cagtcaaatt gcagtactct gcgggtgtat acagaatagc agaatgggca gacattacga
     9901 atgcacacgg tgtggtgggc ccaggtattg ttagcggttt gaagcaggcg gcagaagaag
     9961 taacaaagga acctagaggc cttttgatgt tagcagaatt gtcatgcaag ggctccctat
    10021 ctactggaga atatactaag ggtactgttg acattgcgaa gagcgacaaa gattttgtta
    10081 tcggctttat tgctcaaaga gacatgggtg gaagagatga aggttacgat tggttgatta
    10141 tgacacccgg tgtgggttta gatgacaagg gagacgcatt gggtcaacag tatagaaccg
    10201 tggatgatgt ggtctctaca ggatctgaca ttattattgt tggaagagga ctatttgcaa
    10261 agggaaggga tgctaaggta gagggtgaac gttacagaaa agcaggctgg gaagcatatt
    10321 tgagaagatg cggccagcaa aactaaaaaa ctgtattata agtaaatgca tgtatactaa
    10381 actcacaaat tagagcttca atttaattat atcagttatt acccgggaat ctcggtcgta
    10441 atgattttta taatgacgaa aaaaaaaaaa ttggaaagaa aacccccccc cccgcagcgt
    10501 tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg
    10561 gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga
    10621 ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt
    10681 ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat
    10741 cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca
    10801 ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc
    10861 acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc
    10921 tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt
    10981 gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac
    11041 gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct
    11101 tcacgaccac gctgatgagc tttaccgctg ggccattctc atgaagaata tcttgaattt
    11161 attgtcatat tactagcacg tgcccatcac atatacctgc cgttcactat tatttagtga
    11221 aatgagatat tatgatattt tctgaattgt gattaaaaag gcaactttat gcccatgcaa
    11281 cagaaactat aaaaaataca gagaatgaaa agaaacagat agatttttta gttctttagg
    11341 cccgtagtct gcaaatcctt ttatgatttt ctatcaaaca aaagaggaaa atagaccagt
    11401 tgcaatccaa acgagagtct aatagaatga ggtcgaaaag taaatcgcgc gggtttgtta
    11461 ctgataaagc aggcaagacc taaaatgtgt aaagggcaaa gtgtatactt tggcgtcacc
    11521 ccttacatat tttaggtctt tttttattgt gcgtaactaa cttgccatct tcaaacagga
    11581 gggctggaag aagcagaccg ctaacacagt acataaaaaa ggagacatga acgatgaaca
    11641 tcaaaaagtt tgcaaaacaa gcaacagtat taacctttac taccgcactg ctggcaggag
    11701 gcgcaactca agcgtttgcg aaagaaacga accaaaagcc atataaggaa acatacggca
    11761 tttcccatat tacacgccat gatatgctgc aaatccctga acagcaaaaa aatgaaaaat
    11821 atcaagtttc tgaatttgat tcgtccacaa ttaaaaatat ctcttctgca aaaggcctgg
    11881 acgtttggga cagctggcca ttacaaaacg ctgacggcac tgtcgcaaac tatcacggct
    11941 accacatcgt ctttgcatta gccggagatc ctaaaaatgc ggatgacaca tcgatttaca
    12001 tgttctatca aaaagtcggc gaaacttcta ttgacagctg gaaaaacgct ggccgcgtct
    12061 ttaaagacag cgacaaattc gatgcaaatg attctatcct aaaagaccaa acacaagaat
    12121 ggtcaggttc agccacattt acatctgacg gaaaaatccg tttattctac actgatttct
    12181 ccggtaaaca ttacggcaaa caaacactga caactgcaca agttaacgta tcagcatcag
    12241 acagctcttt gaacatcaac ggtgtagagg attataaatc aatctttgac ggtgacggaa
    12301 aaacgtatca aaatgtacag cagttcatcg atgaaggcaa ctacagctca ggcgacaacc
    12361 atacgctgag agatcctcac tacgtagaag ataaaggcca caaatactta gtatttgaag
    12421 caaacactgg aactgaagat ggctaccaag gcgaagaatc tttatttaac aaagcatact
    12481 atggcaaaag cacatcattc ttccgtcaag aaagtcaaaa acttctgcaa agcgataaaa
    12541 aacgcacggc tgagttagca aacggcgctc tcggtatgat tgagctaaac gatgattaca
    12601 cactgaaaaa agtgatgaaa ccgctgattg catctaacac agtaacagat gaaattgaac
    12661 gcgcgaacgt ctttaaaatg aacggcaaat ggtacctgtt cactgactcc cgcggatcaa
    12721 aaatgacgat tgacggcatt acgtctaacg atatttacat gcttggttat gtttctaatt
    12781 ctttaactgg cccatacaag ccgctgaaca aaactggcct tgtgttaaaa atggatcttg
    12841 atcctaacga tgtaaccttt acttactcac acttcgctgt acctcaagcg aaaggaaaca
    12901 atgtcgtgat tacaagctat atgacaaaca gaggattcta cgcagacaaa caatcaacgt
    12961 ttgcgccgag cttcctgctg aacatcaaag gcaagaaaac atctgttgtc aaagacagca
    13021 tccttgaaca aggacaatta acagttaaca aataaa
//
