LOCUS       sequence-422694-        6719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses fl. MBD2 RFP tagged in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6719)
  AUTHORS   Becker A, Allmann L, Hofstatter M, Casa V, Weber P, Lehmkuhl A, 
            Herce HD, Cardoso MC
  TITLE     Direct homo- and hetero-interactions of MeCP2 and MBD2.
  JOURNAL   PLoS One. 2013;8(1):e53730. doi: 10.1371/journal.pone.0053730. Epub 
            2013 Jan 15.
  PUBMED    23335972
REFERENCE   2  (bases 1 to 6719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e53730. doi"; pages: " 
            10.1371/journal.pone.0053730. Epub 2013 Jan 15"
COMMENT     Sequence Label: pFB-MBD2mR (pc2519)
FEATURES             Location/Qualifiers
     source          1..6719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      35..489
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(122..141)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     331..352
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        515..619
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(620..688,689..1480)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(838..857)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1651..2239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2140..2159
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2393..2410
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2544..2768
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2835..3368)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3538..3543,3544..3560,3561..3566))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3918..4009
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3934..3951
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             5374..6048
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             5374..6048
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(5504..5522)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5772..5791
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5966..5989
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    6242..6376
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6279..6298)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6333..6352
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  6405..6570
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 tcaacgctta tttgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
       61 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
      121 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
      181 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
      241 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
      301 cctttgacgt tggagtccac gttcttaata gtggactctt gttccaaact ggaacaacac
      361 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
      421 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
      481 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      541 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
      601 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
      661 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
      721 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
      781 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
      841 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
      901 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
      961 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     1021 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     1081 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     1141 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     1201 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     1261 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     1321 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     1381 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     1441 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     1501 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     1561 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     1621 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     1681 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     1741 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1801 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1861 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1921 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1981 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2041 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2101 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2161 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2221 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2281 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2341 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2401 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     2461 tatttcacac cgcatagacc agccgcgtaa cctggcaaaa tcggttacgg ttgagtaata
     2521 aatggatgcc ctgcgtaagc gggtgtgggc ggacaataaa gtcttaaact gaacaaaata
     2581 gatctaaact atgacaataa agtcttaaac tagacagaat agttgtaaac tgaaatcagt
     2641 ccagttatgc tgtgaaaaag catactggac ttttgttatg gctaaagcaa actcttcatt
     2701 ttctgaagtg caaattgccc gtcgtattaa agaggggcgt ggccaagggc atggtaaaga
     2761 ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa gccgatctcg
     2821 gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc
     2881 cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg
     2941 tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg
     3001 gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta
     3061 cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg
     3121 tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag gtaatcggag
     3181 tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa aagatcaaga
     3241 gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat gatgcccata
     3301 cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc
     3361 cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata
     3421 gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc gttaccaccg
     3481 ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt gcattacagt
     3541 ttacgaaccg aacaggctta tgtcaactgg gttcgtgcct tcatccgttt ccacggtgtg
     3601 cgtcacccgg caaccttggg cagcagcgaa gtcgaggcat ttctgtcctg gctggcgaac
     3661 gagcgcaagg tttcggtctc cacgcatcgt caggcattgg cggccttgct gttcttctac
     3721 ggcaaggtgc tgtgcacgga tctgccctgg cttcaggaga tcggaagacc tcggccgtcg
     3781 cggcgcttgc cggtggtgct gaccccggat gaagtggttc gcatcctcgg ttttctggaa
     3841 ggcgagcatc gtttgttcgc ccaggactct agctatagtt ctagtggttg gctacgtata
     3901 ctccggaata ttaatagatc atggagataa ttaaaatgat aaccatctcg caaataaata
     3961 agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata ttccggatta
     4021 ttcataccgt cccaccatcg ggcgcggatc ccggtccgaa gcgcgcggaa ttcaaaggcc
     4081 tacgtcgacg ccatgcgcgc gcacccgggg ggaggccgct gctgcccgga gcaggaggag
     4141 ggggagagcg cggcgggcgg cagcggcgct ggcggcgact ccgccataga gcaggggggc
     4201 cagggcagcg cgctcgctcc gtccccggtg agcggcgtgc gcagggaagg cgctcggggc
     4261 ggcggccgtg gccgggggcg gtggaagcag gcggcccggg gcggcggcgt ctgtggccgt
     4321 ggccgtggcc gtggccgggg tcggggccgt ggccggggcc ggggccgggg ccgcggccgt
     4381 ccccagagtg gcggcagcgg ccttggcggc gacggcggcg gcggcgcggg cggctgcggc
     4441 gtcggcagcg gtggcggcgt cgccccccgg cgggatcctg tccctttccc gtcggggagc
     4501 tcggggccgg ggcccagggg accccgggcc acggagagcg ggaagaggat ggactgcccg
     4561 gccctccccc ccggatggaa gaaggaggaa gtgatccgaa aatcagggct cagtgctggc
     4621 aagagcgatg tctactactt cagtccaagt ggtaagaagt tcagaagtaa acctcagctg
     4681 gcaagatacc tgggaaatgc tgttgacctt agcagttttg acttcaggac cggcaagatg
     4741 atgcctagta aattacagaa gaacaagcag agactccgga atgaccccct caatcagaac
     4801 aagggtaaac cagacctgaa cacaacattg ccaattagac aaactgcatc aattttcaag
     4861 caaccagtaa ccaaattcac gaaccacccg agcaataagg tgaagtcaga cccccagcgg
     4921 atgaatgaac aaccacgtca gcttttctgg gagaagaggc tacaaggact tagcgcatca
     4981 gatgtaacag aacaaattat aaaaaccatg gagctaccta aaggtcttca aggagtcggt
     5041 ccaggtagca atgacgagac ccttctgtct gctgtggcca gtgctttaca cacaagctct
     5101 gcgcccatca caggacaagt ctctgctgcc gtggaaaaga accctgctgt ttggcttaac
     5161 acatctcaac ccctctgcaa agctttcatt gttacagatg aagacattag gaaacaggaa
     5221 gagcgagtcc aacaagtacg caagaaactg gaggaggcac tgatggccga catcctgtcc
     5281 cgggctgcgg acacggagga agtagacatt gacatggacg atggagatga ggcgtgcggc
     5341 ccggtaccgc gggcccggga tccaccggtc gacatggcct cctccgagga cgtcatcaag
     5401 gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga gttcgagatc
     5461 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     5521 aagggcggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcca gtacggctcc
     5581 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     5641 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     5701 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     5761 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc caccgagcgg
     5821 atgtaccccg aggacggcgc cctgaagggc gagatcaaga tgaggctgaa gctgaaggac
     5881 ggcggccact acgacgccga ggtcaagacc acctacatgg ccaagaagcc cgtgcagctg
     5941 cccggcgcct acaagaccga catcaagctg gacatcacct cccacaacga ggactacacc
     6001 atcgtggaac agtacgagcg cgccgagggc cgccactcca ccggcgccgg cctgtacaag
     6061 taaagcggcc gctttcgaat ctagagcctg cagtctcgag gcatgcggta ccaagcttgt
     6121 cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
     6181 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     6241 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6301 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6361 ttatcatgtc tggatctgat cactgcttga gcctaggaga tccgaaccag ataagtgaaa
     6421 tctagttcca aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca
     6481 gttcccatct attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct
     6541 cccagttccc aactattttg tccgcccaca gcggggcatt tttcttcctg ttatgttttt
     6601 aatcaaacat cctgccaact ccatgtgaca aaccgtcatc ttcggctact ttttctctgt
     6661 cacagaatga aaatttttct gtcatctctt cgttattaat gtttgtaatt gactgaata
//
