LOCUS       sequence-422703-        1956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial protein expression of Pacer RH domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Tudorica DA, Basak B, Puerta Cordova AS, Khuu G, Rose K, Lazarou M, 
            Holzbaur ELF, Hurley JH
  TITLE     A RAB7A phosphoswitch coordinates Rubicon Homology protein 
            regulation of Parkin-dependent mitophagy.
  JOURNAL   J Cell Biol. 2024 Jul 1;223(7):e202309015. doi: 
            10.1083/jcb.202309015. Epub 2024 May 10.
  PUBMED    38728007
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2024-07-1"; volume: "223(7):e202309015. doi"; pages: 
            " 10.1083/jcb.202309015. Epub 2024 May 10"
COMMENT     Sequence Label: 2CT-PacerRH
FEATURES             Location/Qualifiers
     source          1..1956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..1140
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     primer_bind     1114..1137
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1195..1215
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgaaatctt ctcaccatca ccatcaccat ggttcttcta tgaaaatcga agaaggtaaa
       61 ctggtaatct ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa
      121 ttcgagaaag ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa
      181 ttcccacagg ttgcggcaac tggcgatggc cctgacatta tcttctgggc acacgaccgc
      241 tttggtggct acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag
      301 gacaagctgt atccgtttac ctgggatgcc gtacgttaca acggcaagct gattgcttac
      361 ccgatcgctg ttgaagcgtt atcgctgatt tataacaaag atctgctgcc gaacccgcca
      421 aaaacctggg aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg
      481 ctgatgttca acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt
      541 tatgcgttca agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct
      601 ggcgcgaaag cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca
      661 gacaccgatt actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc
      721 aacggcccgt gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta
      781 ctgccgacct tcaagggtca accatccaaa ccgttcgttg gcgtgctgag cgcaggtatt
      841 aacgccgcca gtccgaacaa agagctggca aaagagttcc tcgaaaacta tctgctgact
      901 gatgaaggtc tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct
      961 tacgaggaag agttggcgaa agatccacgt attgccgcca ctatggaaaa cgcccagaaa
     1021 ggtgaaatca tgccgaacat cccgcagatg tccgctttct ggtatgccgt gcgtactgcg
     1081 gtgatcaacg ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact
     1141 aattcgagct cgaacaacaa caacaataac aataacaaca acctcgggat cgaggaaaac
     1201 ctgtacttcc aatccaatgc aagggacctg gtggtggccg ctcagaactt cttctgcgct
     1261 ggttgtggaa cccctgtgga gcctaagttc gtgaagagac tgcgttactg cgaatacctg
     1321 ggaaagtact tctgcgactg ctgccactcc tacgctgagt cttgcatccc agcccgcatc
     1381 ctgatgatgt gggacttcaa gaagtactac gtgtccaact tctctaagca gctgctggac
     1441 agcatctggc accagcccat cttcaacctg ctgagcatcg gacagtccct gtacgccaag
     1501 gctaaggaac tggacagagt gaaggagatc caggaacagc tgttccacat caagaagctg
     1561 ctgaagacct gccgtttcgc caactctgct ctgaaggagt tcgagcaggt gcctggccac
     1621 ctgaccgacg agctgcacct gttcagcctg gaagacctgg tgagaatcaa gaagggcctg
     1681 ctggctccac tgctgaagga catcctgaag gccagcctgg ctcacgtggc tggatgtgag
     1741 ctgtgccagg gcaagggctt catctgcgag ttctgccaga acaccaccgt gatcttcccc
     1801 ttccagactg ctacctgccg ccgctgctcc gcctgccgcg cttgcttcca caagcagtgc
     1861 ttccagagca gcgagtgccc tagatgtgcc cgtattactg ctcgtagaaa actcctggaa
     1921 agtgtcgcct ccgccgccac cattggaagt ggataa
//
