LOCUS       sequence-422714-        7909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GFP-tagged BRD protein, which can be used to be expressed in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M,
            Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM
  TITLE     Screen identifies bromodomain protein ZMYND8 in chromatin 
            recognition of transcription-associated DNA damage that promotes 
            homologous recombination.
  JOURNAL   Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.
  PUBMED    25593309
REFERENCE   2  (bases 1 to 7909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.252189"; journalName: "Genes Dev"; date: "2015-01-15- 
            15"; volume: "29"; issue: "2"; pages: "197-211"
COMMENT     Sequence Label: GFP-BRD7
FEATURES             Location/Qualifiers
     source          1..7909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        93..472
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        473..676
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     627..647
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     673..697
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(801..803,804..806,807..1517)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(846..867)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1107..1126)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1454..1475
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    1533..1557
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3592..3616)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3624..3665
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(3705..3724)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3749..3797
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3999..4427
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4086..4105)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4296..4317
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4436..4456)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4441..4770
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4621..4756
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4683..4702
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4818..4865
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4884..5282
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5440..5561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5477..5496)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5531..5550
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5610..5626)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5610..5626)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5623..5645)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5634..5650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5658..5664,5665..5682,5683..5688))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5703..5724
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5841..5858)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6012..6600)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6092..6111)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6771..7562,7563..7631))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7394..7413
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7632..7736)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7811..7830)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
       61 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
      121 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      181 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      241 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      301 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      361 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      421 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      481 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      541 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      601 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      661 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
      721 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag aggatcccta
      781 ccggtgatat cctcgagacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
      841 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
      901 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      961 tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag tgcttcgccc
     1021 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     1081 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     1141 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     1201 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaag gtctatatca
     1261 ccgccgacaa gcagaagaac ggcatcaagg tgaacttcaa gacccgccac aacatcgagg
     1321 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     1381 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     1441 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     1501 tggacgagct gtacaagggc tcgagcccat caacaagttt gtacaaaaaa gcaggcttcg
     1561 aaggagatag aaccaattct ctaaggaaat acttaaccat ggtcgactgg atccggtacc
     1621 tcatgggcaa gaagcacaag aagcacaagt cggacaaaca cctctacgag gagtatgtag
     1681 agaagccctt gaagctggtc ctcaaagtag gagggaacga agtcaccgaa ctctccacgg
     1741 gcagctcggg gcacgactcc agcctcttcg aagacaaaaa cgatcatgac aaacacaagg
     1801 acagaaagcg gaaaaagaga aagaaaggag agaagcagat tccaggggaa gaaaagggga
     1861 gaaaacggag aagagttaag gaggataaaa agaagcgaga tcgagaccgg gtggagaatg
     1921 aggcagaaaa agatctccag tgtcacgccc ctgtgagatt agacttgcct cctgagaagc
     1981 ctctcacaag ctctttagcc aaacaagaag aagtagaaca gacacccctt caagaagctt
     2041 tgaatcaact gatgagacaa ttgcagagaa aagatccaag tgctttcttt tcatttcctg
     2101 tgactgattt tattgctcct ggctactcca tgatcattaa acacccaatg gattttagta
     2161 ccatgaaaga aaagatcaag aacaatgact atcagtccat agaagaacta aaggataact
     2221 tcaaactaat gtgtactaat gccatgattt acaataaacc agagaccatt tattataaag
     2281 ctgcaaagaa gctgttgcac tcaggaatga aaattcttag ccaggaaaga attcagagcc
     2341 tgaagcagag catagacttc atggctgact tgcagaaaac tcgaaagcag aaagatggaa
     2401 cagacacctc acagagtggg gaggacggag gctgctggca gagagagaga gaggactctg
     2461 gagatgccga agcacacgcc ttcaagagtc ccagcaaaga aaataaaaag aaagacaaag
     2521 atatgcttga agataagttt aaaagcaata atttagagag agagcaggag cagcttgacc
     2581 gcatcgtgaa ggaatctgga ggaaagctga ccaggcggct tgtgaacagt cagtgcgaat
     2641 ttgaaagaag aaaaccagat ggaacaacga cgttgggact tctccatcct gtggatccca
     2701 ttgtaggaga gccaggctac tgccctgtga gactgggaat gacaactgga agacttcagt
     2761 ctggagtgaa tactttgcag gggttcaaag aggataaaag gaacaaagtc actccagtgt
     2821 tatatttgaa ttatgggccc tacagttctt atgcaccgca ttatgactcc acatttgcaa
     2881 atatcagcaa ggatgattct gatttaatct attcaaccta tggggaagac tctgatcttc
     2941 caagtgattt cagcatccat gagtttttgg ccacgtgcca agattatccg tatgtcatgg
     3001 cagatagttt actggatgtt ttaacaaaag gagggcattc caggacccta caagagatgg
     3061 agatgtcatt gcctgaagat gaaggccata ctaggacact tgacacagca aaagaaatgg
     3121 agattacaga agtagagcca ccagggcgtt tggactccag tactcaagac aggctcatag
     3181 cgctgaaagc agtaacaaat tttggcgttc cagttgaagt ttttgactct gaagaagctg
     3241 aaatattcca gaagaaactt gatgagacca ccagattgct cagggaactc caggaagccc
     3301 agaatgaacg tttgagcacc agaccccctc cgaacatgat ctgtctcttg ggtccctcat
     3361 acagagaaat gcatcttgct gaacaagtga ccaataatct taaagaactt gcacagcaag
     3421 taactccagg tgatatcgta agcacgtatg gagttcgaaa agcaatgggg atttccattc
     3481 cttcccccgt catggaaaac aactttgtgg atttgacaga agacactgaa gaacctaaaa
     3541 agacggatgt tgctgagtgt ggacctggtg gaagtcactc gagatatcta gacccagctt
     3601 tcttgtacaa agtggttgat aacgggaagc ctatccctaa ccctctcctc ggtctcgatt
     3661 ctacgcgtac cggttagtaa tgagtttaaa cgggggaggc taactgaaac acggaaggag
     3721 acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa aacgcacggg
     3781 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     3841 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     3901 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     3961 agcagatctg cgcagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     4021 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4081 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4141 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4201 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4261 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4321 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4381 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     4441 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     4501 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     4561 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     4621 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     4681 tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga
     4741 ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc
     4801 ggatctgatc agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat
     4861 acgacaaggt gaggaactaa accatggcca agcctttgtc tcaagaagaa tccaccctca
     4921 ttgaaagagc aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca
     4981 gcgcagctct ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg
     5041 ggggaccttg tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc
     5101 tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt
     5161 gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg
     5221 gacagccgac ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct
     5281 aagcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     5341 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     5401 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     5461 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     5521 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     5581 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     5641 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     5701 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     5761 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5821 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5881 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5941 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     6001 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     6061 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     6121 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     6181 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     6241 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     6301 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     6361 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6421 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6481 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6541 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6601 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6661 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6721 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6781 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6841 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6901 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6961 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     7021 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     7081 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     7141 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     7201 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     7261 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     7321 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     7381 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7441 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7501 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7561 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7621 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7681 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7741 tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt
     7801 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt
     7861 gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaac
//
