LOCUS       sequence-422735-        9570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express LucN-PRPF6 fusion protein in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   Maita H, Tomita K, Ariga H
  TITLE     A split luciferase-based reporter for detection of a cellular 
            macromolecular complex.
  JOURNAL   Anal Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015.
  PUBMED    24503441
REFERENCE   2  (bases 1 to 9570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2014 Feb 3. pii: S0003-2697(14)00035-9. doi: 
            10.1016/j.ab.2014.01.015."
COMMENT     Sequence Label: pSplit2-NO-PRPF6
FEATURES             Location/Qualifiers
     source          1..9570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(49..68)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        240..619
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        620..823
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     774..794
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     868..887
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        868..886
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             909..932
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             969..992
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(1077..1095)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             complement(2212..2238)
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHYHHHH"
     primer_bind     complement(5166..5183)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5172..5396
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5442..5870
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5529..5548)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5739..5760
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5879..5899)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5884..6213
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6064..6199
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6126..6145
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6280..7074
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6334..6353)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6944..6963
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7248..7369
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7285..7304)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7339..7358
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7418..7434)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7418..7434)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7431..7453)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7442..7458
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7466..7472,7473..7490,7491..7496))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7511..7532
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7649..7666)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7820..8408)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7900..7919)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8579..9370,9371..9439))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9202..9221
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9440..9544)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acgtcgacgg atcgggagat ctcccgatcc cctatggtgc actctcagta caatctgctc
       61 tgatgccgca tagttaagcc agtatctgct ccctgcttgt gtgttggagg tcgctgagta
      121 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
      181 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg
      241 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
      301 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      361 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      421 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      481 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      541 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      601 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      661 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      721 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      781 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactagaga
      841 acccactgct tactggctta tcgaaattaa tacgactcac tatagggtct agacccacca
      901 tggctagctg gagccacccg cagttcgaga aaggtggagg ttccggaggt ggatcgggag
      961 gtggatcgtg gagccacccg cagttcgaaa aaggcgccga gaccgcggga atggaagacg
     1021 ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga accgctggag
     1081 agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt gcttttacag
     1141 atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc gttcggttgg
     1201 cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta tgcagtgaaa
     1261 actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc
     1321 ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt tcgcagccta
     1381 ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac
     1441 caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga
     1501 tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat tttgtaccag
     1561 agtcctttga tcgtgacaaa acaattgcac tgataatgaa ctcctctgga tctactgggt
     1621 tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg catgccagag
     1681 atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt gttccattcc
     1741 atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt cgagtcgtct
     1801 taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac aaaattcaaa
     1861 gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg attgacaaat
     1921 acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg aaagaagtcg
     1981 gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat gggctcactg
     2041 agactacatc agctattctg attacacccg agggggatga taaaccgggc gcggtcggta
     2101 aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa acgctgggcg
     2161 ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgggtacc ggtggtggtg
     2221 gtggtagtgg tggtggtgga tccgaattca tgaacaagaa gaagaaaccg ttcctaggga
     2281 tgcccgcgcc cctcggctac gtgccggggc tgggccgggg cgccactggc ttcaccacgc
     2341 ggtcagacat tgggcccgcc cgtgatgcaa atgaccctgt ggatgatcgc catgcacccc
     2401 caggcaagag aaccgttggg gaccagatga agaaaaatca ggctgctgac gatgacgacg
     2461 aggatctaaa tgacaccaat tacgatgagt ttaatggcta tgctgggagc ctcttctcaa
     2521 gtggacccta cgagaaagat gatgaggaag cagatgctat ctatgcagcc ctggataaaa
     2581 ggatggatga aagaagaaaa gaaagacggg agcaaaggga gaaagaagaa atagagaaat
     2641 atcgtatgga acgccccaaa atccaacagc agttctcaga cctcaagagg aagttggcag
     2701 aagtcacaga agaagagtgg ctgagcatcc ccgaggttgg cgatgccaga aataaacgtc
     2761 agcggaaccc acgctatgag aagctgaccc ctgttcctga cagtttcttt gccaaacatt
     2821 tacagaccgg agagaaccat acctcagtgg atccccgaca aactcaattt ggaggtctta
     2881 acacacccta tccaggtgga ctaaacactc catacccagg tggaatgacg ccaggactga
     2941 tgacacctgg cacaggtgag ctggacatga ggaagattgg ccaagcgagg aacactctga
     3001 tggacatgag gctgagccag gtgtctgact ccgtgagtgg acagaccgtc gttgacccca
     3061 aaggctacct gacggattta aattccatga tcccgacaca cggaggagac atcaatgata
     3121 tcaagaaggc gcgactgctc ctcaagtctg ttcgggagac gaaccctcat cacccgccag
     3181 cctggattgc atcagcccgc ctggaagaag tcactgggaa gctacaagta gctcggaacc
     3241 ttatcatgaa ggggacggag atgtgcccca agagtgaaga tgtctggctg gaagcagcca
     3301 ggttgcagcc tggggacaca gccaaggccg tggtagccca agctgtccgt catctcccac
     3361 agtctgtcag gatttacatc agagccgcag agctggaaac ggacattcgt gcaaagaagc
     3421 gggttcttcg gaaagccctc gagcatgttc caaactcggt tcgcttgtgg aaagcagccg
     3481 ttgagctgga agaacctgaa gatgctagaa tcatgctgag ccgagctgtg gagtgctgcc
     3541 ccaccagcgt ggagctctgg cttgctctgg caaggctgga gacctatgaa aatgcccgca
     3601 aggtcttgaa caaggcgcgg gagaacattc ctacagaccg acatatctgg atcacggctg
     3661 ctaagctgga ggaagccaat gggaacacgc agatggtgga gaagatcatc gaccgagcca
     3721 tcacctcgct gcgggccaac ggtgtggaga tcaaccgtga gcagtggatc caggatgccg
     3781 aggaatgtga cagggctggg agtgtggcca cctgccaggc cgtcatgcgt gccgtgattg
     3841 ggattgggat tgaggaggaa gatcggaagc atacctggat ggaggatgct gacagttgtg
     3901 tagcccacaa tgccctggag tgtgcacgag ccatctacgc ctacgccctg caggtgttcc
     3961 ccagcaagaa gagtgtgtgg ctgcgcgccg cgtacttcga gaagaaccat ggcactcggg
     4021 agtccctgga agcactcctg cagagggctg tggcccactg ccccaaagca gaggtgctgt
     4081 ggctcatggg cgccaagtcc aagtggctgg caggggatgt gcctgcagca aggagcatcc
     4141 tggccctggc cttccaggcc aaccccaaca gtgaggagat ctggctggca gccgtgaagc
     4201 tggagtccga gaatgatgag tacgagcggg cccggaggct gctggccaag gcgcggagca
     4261 gtgcccccac cgcccgggtg ttcatgaagt ctgtgaagct ggagtgggtg caagacaaca
     4321 tcagggcagc ccaagatctg tgcgaggagg ccctgcggca ctatgaggac ttccccaagc
     4381 tgtggatgat gaaggggcag atcgaggagc agaaggagat gatggagaag gcgcgggaag
     4441 cctataacca ggggttgaag aagtgtcccc actccacacc cctgtggctt ttgctctctc
     4501 ggctggagga gaagattggg cagcttactc gagcacgggc cattttggaa aagtctcgtc
     4561 tgaagaaccc aaagaaccct gggctgtggt tggagtccgt gcggctggag taccgtgcgg
     4621 ggctgaagaa catcgcaaat acactcatgg ccaaggcgct gcaggagtgc cccaactccg
     4681 gtatcctgtg gtctgaggcc atcttcctcg aggcaaggcc ccagaggagg accaagagcg
     4741 tggatgccct gaagaagtgt gagcatgacc cccatgtgct cctggccgtg gccaagctgt
     4801 tttggagtca gcggaagatc accaaggcca gggagtggtt ccaccgcact gtgaagattg
     4861 actcggacct gggggatgcc tgggccttct tctacaagtt tgagctgcag catggcactg
     4921 aggagcagca ggaggaggtg aggaagcgct gtgagagtgc agagcctcgg catggggagc
     4981 tgtggtgcgc cgtgtccaag gacatcgcca actggcagaa gaagatcggg gacatcctta
     5041 ggctggtggc cggccgcatc aagaacacct tctgagtcga ggtcgacctg cagggggacc
     5101 atggtctctg atatctaact aagcttgcgg ccgcagatct agcttaagtt taaaccgctg
     5161 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5221 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5281 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     5341 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     5401 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     5461 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5521 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5581 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5641 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5701 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5761 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     5821 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     5881 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5941 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     6001 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     6061 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6121 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     6181 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     6241 ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     6301 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     6361 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     6421 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta
     6481 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     6541 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     6601 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     6661 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     6721 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     6781 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc
     6841 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     6901 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     6961 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     7021 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     7081 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     7141 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     7201 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg
     7261 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7321 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta
     7381 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7441 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7501 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7561 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     7621 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     7681 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     7741 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     7801 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7861 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7921 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7981 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8041 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8101 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8161 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8221 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8281 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8341 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8401 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     8461 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     8521 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     8581 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8641 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8701 gtgctgcaat gataccgcga gaaccacgct caccggctcc agatttatca gcaataaacc
     8761 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8821 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8881 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8941 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9001 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9061 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9121 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9181 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9241 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9301 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9361 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9421 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9481 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     9541 cgcgcacatt tccccgaaaa gtgccacctg
//
