LOCUS       sequence-422738-        5879 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SRP14 and SRP9 proteins as a fusion protein in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5879)
  TITLE     Strub Lab 14-9VN plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: p14-9VN
FEATURES             Location/Qualifiers
     source          1..5879
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     219..239
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    263..885
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     667..686
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     CDS             complement(join(896..1408,1409..1411,1412..1414))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     1089..1108
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1348..1369
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1411..1420
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     RBS             1867..1875
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(2147..2170)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(2204..2407)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2233..2253)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2408..2787
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(2948..2965)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2948..2964)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2957..2979)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(3105..3560)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3242..3263)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3454..3473
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3587..3691
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3692..3760,3761..4552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3910..3929)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4723..5311
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5212..5231
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5465..5482
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5599..5620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5635..5640,5641..5658,5659..5665)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5673..5689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5678..5700
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5697..5713
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5697..5713
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5727..5746
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5727..5745
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(join(5784..5879,1..234))
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(5798..5879,1..54)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
       61 agttaggggc gggactatgg ttgctgacta attgagatgc atgctttgca tacttctgcc
      121 tgctggggag cctggggact ttccacacct ggttgctgac taattgagat gcatgctttg
      181 catacttctg cctgctgggg agcctgggga ctttccacac cctaactgac acacattcca
      241 cagccaagct ggccgcgtac ccaattcaac aggcatctac tgagtggacc caacgcatga
      301 gaggacagtg ccaagcaagc aactcaaatg tcccaccggt tgggcatggc caggtagcct
      361 atgctgtgtc tggacgtcct cctgctggta tagttatttt aaaatcagaa ggacagggaa
      421 gggagcagtg gttcacgcct gtaatcccag caatttggga ggccaaggtg ggtagatcac
      481 ctgagattag gagttggaga ccagcctggc caatatggtg aaaccccgtc tctaccaaaa
      541 aaacaaaaat tagctgagcc tggtcatgca tgcctggaat cccaacaact cgggaggctg
      601 aggcaggaga atcgcttgaa cccaggaggc ggagattgca gtgagccaag attgtgccac
      661 tgcactccag cttggttccc aatagacccc gcaggcccta caggttgtct tcccaacttg
      721 ccccttgctc cataccaccc ccctccaccc cataatatta tagaaggaca cctagtcaga
      781 caaaatgatg caacttaatt ttattaggac aaggctggtg ggcactggag tggcaacttc
      841 cagggccagg agaggcactg gggaggggtc acagggatgc cacccgggat ccctactcga
      901 tgttgtggcg gatcttgaag ttggccttga tgccgttctt ctgcttgtcg gcggtgatat
      961 agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
     1021 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
     1081 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
     1141 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcgggcga
     1201 agcactgcag gccgtagccc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
     1261 cggtggtgca gatcagcttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
     1321 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
     1381 ccccggtgaa cagctcctcg cccttgctca ccatggtggc gatggatctt ctagagtcga
     1441 ctggtacccc ctcagtttcc atggtaacat tgcgggcttc cttggctacc ataagtcgca
     1501 ttagttgact gtggaatttc tcaatcttct ttacatcttg agcttggtct gttttataca
     1561 ccaaacaaac taaatcatct gttgctttaa cacacaagtt cccatcagaa tgcctatatt
     1621 tgagaaccac acgtgccttc atagggtcag cgaggtaaag cttctcggca gcgcggctga
     1681 actcctccca ggtctggtac tgcggcattc ccccggggga ctgtgctgct gttgctgctg
     1741 ttgttgctgc tgttgttgct gctgttgctg ctgcggcagg tgctgctgct gctgctgttg
     1801 ctgctgcttt ggtcttctta gttttgttct ttttgtctct cttcttcaac ccatccatgt
     1861 tagctctaag gaggtttgaa taagccatct gaaacttatt cacttccttg gagctcacca
     1921 cagtgctgat cttcttcttc ccatcggtag ctcttaacag acacttgttg tctgcgggct
     1981 caaagccctc cacagtacct ttctttggaa tgggtttggt tcgaccgtca tacttcttca
     2041 aggtgatata gacgctgccc gacgtccgac acttctggaa aagtctggtc agctccgtca
     2101 ggaactgctc gctctccaac aacaccatag aattcgcggc cgcaagcttg tcgtcatcgt
     2161 ctttgtagtc catggtagat caattctgac ggttcactaa acgagctctg cttatataga
     2221 cctcccaccg tacacgccta ccgcccattt gcgtcaacgg ggcggggtta ttacgacatt
     2281 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
     2341 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attggtgtac tgccaaaacc
     2401 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     2461 gtaaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     2521 ggggcggact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     2581 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     2641 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     2701 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     2761 attaataact agtcaataat caatgtcaac atggcggtca tattggacat gagccaatat
     2821 aaatgtacat attatgatat agatacaacg tatgcaatgg ccaatagcca atattgattt
     2881 atgctatata accaatgact aatatggcta attgccaata ttgattcaat gtatagatca
     2941 gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     3001 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     3061 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggaaattg taagcgttaa
     3121 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     3181 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     3241 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     3301 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     3361 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     3421 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     3481 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     3541 tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     3601 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     3661 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     3721 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3781 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3841 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3901 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3961 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4021 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4081 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4141 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4201 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4261 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4321 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4381 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4441 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4501 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     4561 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     4621 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     4681 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4741 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4801 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4861 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4921 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4981 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5041 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5101 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5161 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5221 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5281 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5341 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5401 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5461 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     5521 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     5581 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     5641 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     5701 aaacagctat gacatgatta cgaatttaat acgactcact atagggaatt aactttctgg
     5761 tttttcagtt cctcgaggag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     5821 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaat
//
