LOCUS       sequence-422740-       13525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  genomic integration of genes (inserted in I-SceI-site) via 
            rrn-interposon with TcR and LacZ.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13525)
  AUTHORS   Weihmann R, Kubicki S, Bitzenhofer NL, Domrose A, Bator I, Kirschen 
            LM, Kofler F, Funk A, Tiso T, Blank LM, Jaeger KE, Drepper T, Thies 
            S, Loeschcke A
  TITLE     The modular pYT vector series employed for chromosomal gene 
            integration and expression to produce carbazoles and glycolipids in 
            P. putida.
  JOURNAL   FEMS Microbes. 2022 Dec 19;4:xtac030. doi: 10.1093/femsmc/xtac030. 
            eCollection 2023.
  PUBMED    37333445
REFERENCE   2  (bases 1 to 13525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEMS 
            Microbes."; date: "2022-12-19"; volume: "4:xtac030. doi"; pages: " 
            10.1093/femsmc/xtac030. eCollection 2023"
COMMENT     Sequence Label: pYTRW22K_7Ti1
FEATURES             Location/Qualifiers
     source          1..13525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2170..5244
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYG
                     QDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQ
                     ISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIID
                     ERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIEN
                     GLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLW
                     YTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWS
                     LGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVP
                     KWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSL
                     IKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQ
                     TIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQ
                     LWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELG
                     NKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWK
                     AAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDV
                     EVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPY
                     VFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDG
                     FHMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     complement(2190..2207)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2190..2206)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2199..2221)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(5421..5426,5427..5443,5444..5449)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             5497..6687
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(5849..5868)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     misc_feature    7827..8119
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     rep_origin      8767..9220
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1"
     primer_bind     complement(9248..9270)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    9356..9496
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     9411..9430
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(9511..9528)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9682..10270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9762..9781)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10449..11243)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10560..10579)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     11170..11189
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        11680..11895
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             11896..12699
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
        1 ctagtcccgg gtccacatat ccacgggctg gcaagggagc gcagcgaccg cgcagggcga
       61 agcccggaga gcaagcccgt agggcgccgc agccgccgta ggcggtcacg actttgcgaa
      121 gcaaagtcta gtgagtatac tcaagcattg agtggcccgc cggaggcacc gccttgcgct
      181 gcccccgtcg agccggttgg acaccaaaag ggaggggcag gcatggcggc atacgcgatc
      241 atgcgatgca agaagctggc gaaaatgggc aacgtggcgg ccagtctcaa gcacgcctac
      301 cgcgagcgcg agacgcccaa cgctgacgcc agcaggacgc cagagaacga gcactgggcg
      361 gccagcagca ccgatgaagc gatgggccga ctgcgcgagt tgctgccaga gaagcggcgc
      421 aaggacgctg tgttggcggt cgagtacgtc atgacggcca gcccggaatg gtggaagtcg
      481 gccagccaag aacagcaggc ggcgttcttc gagaaggcgc acaagtggct ggcggacaag
      541 tacggggcgg atcgcatcgt gacggccagc atccaccgtg acgaaaccag cccgcacatg
      601 accgcgttcg tggtgccgct gacgcaggac ggcaggctgt cggccaagga gttcatcggc
      661 aacaaagcgc agatgacccg cgaccagacc acgtttgcgg ccgctgtggc cgatctaggg
      721 ctgcaacggg gcatcgaggg cagcaaggca cgtcacacgc gcattcaggc gttctacgag
      781 gccctggagc ggccaccagt gggccacgtc accatcagcc cgcaagcggt cgagccacgc
      841 gcctatgcac cgcagggatt ggccgaaaag ctgggaatct caaagcgcgt tgagacgccg
      901 gaagccgtgg ccgaccggct gacaaaagcg gttcggcagg ggtatgagcc tgccctacag
      961 gccgccgcag gagcgcgtga gatgcgcaag aaggccgatc aagcccaaga gacggcccga
     1021 gaccttcggg agcgcctgaa gcccgttctg gacgccctgg ggccgttgaa tcgggatatg
     1081 caggccaagg ccgccgcgat catcaaggcc gtgggcgaaa agctgctgac ggaacagcgg
     1141 gaagtccagc gccagaaaca ggcccagcgc cagcaggaac gcgggcgcgc acatttcccc
     1201 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gagacgccac gcgatcgaac
     1261 gtcgttaact ctggcaggca attaaaggga acgtgtatct gacgcaaaga aaactggaaa
     1321 ttggcgagag aatcttctct ctgtctatcg aagactgacc acgcggaggc gtgcgtcttg
     1381 ttagcgtgcg gggtactctt gctatccatt tgagtcacac gacactcgct gttctcgact
     1441 ttacacttgt aagcgacggt attgaacgac gcttctgccc accatcgtta caaccagact
     1501 gatactagat ctatactgtc agccattcag acgaaaccag ccggagatta ccgagcactc
     1561 tattacgtcg gcgaccacta ctgagctagt ggagccgtgg gtaacgatca taccactaag
     1621 aacctctctc gacgcaagcg attacactgc tgtcacatca taatcgtctt gctattcacg
     1681 agttgaccag acactggata gcttgtcact tgaagtattc tgcacgacag ggtgcgtgta
     1741 cctatctgca gcactcaaca gatctccgtc tacttgcgac acctctctta aggtagctgg
     1801 accggattcg ggtctagcct atatcgtcaa tccgttgatc tatgtcgggt gcggagaaag
     1861 aggtaatgaa atgggcgatc gcccacagct tacacagacc taatcgtaga gttcctagta
     1921 atacttagtg gctcgttacc tcgttcttcc aggccagggg gccgtacgcg ctcggcgaac
     1981 agtataagca gattcgactc actagggata acagggtaat ctctgggtat cgactcaatc
     2041 tcgcgctatt gtgctatatc cgtggacggt tgcggtcaat tctgtcggaa aatcaacgga
     2101 tacgaacaat tactcgcgac ttcctcaata cgtgctgcaa cgcgcgcgaa ctttagaagg
     2161 agatatacca tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
     2221 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     2281 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     2341 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc
     2401 gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat
     2461 gcgcccatct acaccaacgt gacctatccc attacggtca atccgccgtt tgttcccacg
     2521 gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa
     2581 ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg
     2641 cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt
     2701 ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gctggagtga cggcagttat
     2761 ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat
     2821 aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc
     2881 cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta
     2941 acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt
     3001 gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa
     3061 aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac
     3121 accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg
     3181 attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt
     3241 cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc
     3301 ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg
     3361 ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa
     3421 acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg
     3481 agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg
     3541 tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa
     3601 tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc
     3661 gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg
     3721 aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc
     3781 gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt
     3841 cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt
     3901 aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg
     3961 aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg
     4021 ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc
     4081 gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg
     4141 gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt
     4201 aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca actctggctc
     4261 acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgcc
     4321 tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc
     4381 atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa taagcgttgg
     4441 caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa aaaacaactg
     4501 ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt
     4561 gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac
     4621 caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt
     4681 acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg gaaaacctac
     4741 cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc gagcgataca
     4801 ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga gcgggtaaac
     4861 tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc ctgttttgac
     4921 cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt
     4981 ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag
     5041 ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg
     5101 cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac
     5161 gactcctgga gcccgtcagt atcggcggaa ttacagctga gcgccggtcg ctaccattac
     5221 cagttggtct ggtgtcaaaa ataacgcgcg cgtaactata acggtcctaa ggtagcgagc
     5281 ttacttgtgc tgttgtgtaa ggacacgctt aacccagaga attccagcga cctattctac
     5341 gactacgcgt ccatggctcg aggagctcgg taccgcccca ctacccgatt tatcaagatt
     5401 tggcggagag tccctcatgt ttgacagctt atcatcgata agctttaatg cggtagttta
     5461 tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc
     5521 gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg
     5581 ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg
     5641 ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac
     5701 cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg
     5761 atcatggcga ccacacccgt cctgtggatc ctctacgccg gacgcatcgt ggccggcatc
     5821 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     5881 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     5941 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     6001 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     6061 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     6121 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     6181 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     6241 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     6301 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     6361 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     6421 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     6481 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg
     6541 atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     6601 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt
     6661 gcatggagcc gggccacctc gacctgagtc gactggcaaa cagctattat gggtattatg
     6721 ggtgtgtatc cagaacgacg gatcttgacg caatttggcg tggctctctt aaggtagccc
     6781 tccgaccaat tgaggtgcgc tctgaccgat cagtttaggg tgagccaacc cggccgatcc
     6841 ggagagacgg gcctcaaagc cgcgtgacga cggctgtggg cccgtaacaa aatccccgca
     6901 ataagctccc gtgagcgtcg gttgaacagc cctggtcggc cccatcagta gcccgaatac
     6961 ttcgctttac gggtcctggg ccggggtgcg ataccttgca gaaatcgagg ccgttcgtta
     7021 attcctgttg cattcgtacc gcctatattt gtctctttgc cggcttatca tcacaagcat
     7081 agcatagcca tttatcggag cgcctccgta cacggtatga tcggacgcct cgtgagatca
     7141 atacgtatac caggtgtcct gtgagcagcg aaagcctata cgcgagatac actgccaaaa
     7201 atccgcgtga ttacgagtcg tggcaaattt ggtctggctg tggtctagac attccaggcg
     7261 gtgcgtctgc tctcgggtgc ctctagtggc tggctagata gactagccgc tggtaaactg
     7321 gtgtggaagt ccatatatcg gtgatcaata tagtggttga catgctggct agtcaacatt
     7381 gagccttttg atcatgcaaa tatattacgg tattttacaa tcaaatatca aacttaacta
     7441 ttgactttat aacttattta ggtggtaaca ttcttataaa aaagaaaaaa attactgcaa
     7501 aacagtacta gcttttaact tgtatcctag gttatctatg ctgtctcacc atagagaata
     7561 ttacctattt cagaatgtat gtccatgatt cgccgggtaa atacatataa tacacaaatc
     7621 tggcttaata aagtctataa tatatctcat aaagaagtgc taaattggct agtgctatat
     7681 atttttaaga aaatttcttt tgactaagtc catatcgact ttgtaaaagt tcacattagc
     7741 atacatatat tacacgagcc agaaatagta acttttgcct aaatcacaaa ttgcaaaatt
     7801 taattgcttg caaaaggtca catgcttata atcaactttt ttaaaaattt aaaatacttt
     7861 tttatttttt atttttaaac ataaatgaaa taatttattt attgtttatg attaccgaaa
     7921 cataaaacct gctcaagaaa aagaaactgt tttgtccttg gaaaaaaagc actacctagg
     7981 agcggccaaa atgccgaggc tttcatagct taaactcttt acagaaaata ggcattatag
     8041 atcagttcga gttttcttat tcttccttcc ggttttatcg tcacagtttt acagtaaata
     8101 agtatcacct cttagagtta acctatacta aaatttatgc cagttaacta tgagataagc
     8161 aagtatcatc tcatttcatt acctgaagtc gagtaaacag aaaatccaat tgttgatgaa
     8221 cctcaatgac ttagaactat ctatcggcag atcatataaa gaggatttag gtactcgaga
     8281 acgctccgta catgcagcta aacttccaag catctctaac ttgggtagtg ctttctccac
     8341 cattttctgt gcttgctcct tcgtggcaag tccagcccat aatgcccaga atgtagttgc
     8401 ggattcgtat gacgttctgt gcttgatttt tgtgttgtag tcaaagaaaa ggtccggtta
     8461 aacggatctc gcattgatga ggcaacgcta attatcaaca tatagattgt tatctatctg
     8521 catgaacacg aaatctttac ttgacgactt gaggctgatg gtgtttatgc aaagaaacca
     8581 ctgtgtttaa tatgtgtcac tgtttgatat tactgtcagc gtagaagata atagtaaaag
     8641 cggttaataa gtgtatttga gataagtgtg ataaagtttt tacagcgaaa agacgataaa
     8701 tacaagaaaa tgattacgag gatacggaga gaggtatgta catgtgtatt tatatactaa
     8761 gctgccggcg gttgtttgca agaccgagaa aaggctagca agaatcgggt cattgtagcg
     8821 tatgcgcctg tgaacattct cttcaacaag tttgattcca ttgcggtgaa atggtaaaag
     8881 tcaaccccct gcgatgtata ttttcctgta caatcaatca aaaagccaaa tgatttagca
     8941 ttatctttac atcttgttat tttacagatt ttatgtttag atcttttatg cttgcttttc
     9001 aaaaggcctg caggcaagtg cacaaacaat acttaaataa atactactca gtaataacct
     9061 atttcttagc atttttgacg aaatttgcta ttttgttaga gtcttttaca ccatttgtct
     9121 ccacacctcc gcttacatca acaccaataa cgccatttaa tctaagcgca tcaccaacat
     9181 tttctggcgt cagtccacca gctaacataa aatgttgcct cgcgcgtttc ggtgatgacg
     9241 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     9301 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     9361 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     9421 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     9481 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     9541 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     9601 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     9661 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     9721 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     9781 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     9841 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     9901 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     9961 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
    10021 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
    10081 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
    10141 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
    10201 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
    10261 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
    10321 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
    10381 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
    10441 gagctcgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
    10501 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
    10561 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
    10621 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
    10681 tgggtcacga cgagatcctc gccgtcgggc atccgcgcct tgagcctggc gaacagttcg
    10741 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
    10801 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
    10861 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
    10921 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
    10981 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
    11041 gatagccgcg ctgcctcgtc ttggagttca ttcagggcac cggacaggtc ggtcttgaca
    11101 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
    11161 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
    11221 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt
    11281 gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc
    11341 ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa
    11401 accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt tctctttgcg
    11461 cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggc gatcgatgga
    11521 agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg
    11581 cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc
    11641 tccagcagcc gcacgcggcg catcgggggg ggggggggtt tcaattcatc attttttttt
    11701 tattcttttt tttgatttcg gtttccttga aatttttttg attcggtaat ctccgaacag
    11761 aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt
    11821 gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa aacctgcagg
    11881 aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt
    11941 cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca
    12001 ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt
    12061 tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag
    12121 ccgctaaagg cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct
    12181 gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg
    12241 gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag
    12301 gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc
    12361 aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac
    12421 aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac
    12481 gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagacgc attgggtcaa
    12541 cagtatagaa ccgtggatga tgtggtctct acaggatctg acattattat tgttggaaga
    12601 ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc
    12661 tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat
    12721 gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt attacccggg
    12781 aatctcggtc gtaatgattt ttataatgac gaaaaaaaaa aaattggaaa gaaaaccccc
    12841 ccccccgcag cgttgggtcc tggccacggg tgcgcatgat cgtgctcctg tcgttgagga
    12901 cccggctagg ctggcggggt tgccttactg gttagcagaa tgaatcaccg atacgcgagc
    12961 gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct
    13021 tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc agcgccctgc accattatgt
    13081 tccggatctg catcgcagga tgctgctggc taccctgtgg aacacctaca tctgtattaa
    13141 cgaagcgctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc cataccgcca
    13201 gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta acccgtatcg
    13261 tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat cccccttaca
    13321 cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc tttatcagaa
    13381 gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa caggcagaca
    13441 tctgtgaatc gcttcacgac cacgctgatg agctttaccg ctgggccatt ctcatgaaga
    13501 atatcttgaa tttattgtca tatta
//
