LOCUS       sequence-422745-        5930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MBD2 (aa1-152) RFP tagged in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Becker A, Allmann L, Hofstatter M, Casa V, Weber P, Lehmkuhl A, 
            Herce HD, Cardoso MC
  TITLE     Direct homo- and hetero-interactions of MeCP2 and MBD2.
  JOURNAL   PLoS One. 2013;8(1):e53730. doi: 10.1371/journal.pone.0053730. Epub 
            2013 Jan 15.
  PUBMED    23335972
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e53730. doi"; pages: " 
            10.1371/journal.pone.0053730. Epub 2013 Jan 15"
COMMENT     Sequence Label: pFB-MBD2.1mR (pc2391)
FEATURES             Location/Qualifiers
     source          1..5930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      16..470
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(103..122)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     312..333
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        496..600
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(601..669,670..1461)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(819..838)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1632..2220
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2121..2140
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2374..2391
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2525..2749
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2816..3349)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3519..3524,3525..3541,3542..3547))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3899..3990
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3915..3932
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4566..5240
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             4566..5240
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(4696..4714)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4964..4983
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     5158..5181
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    5434..5568
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5471..5490)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5525..5544
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5597..5762
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
       61 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      121 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
      181 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
      241 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
      301 cgttcttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
      361 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
      421 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
      481 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      541 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      601 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      661 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
      721 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
      781 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
      841 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
      901 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
      961 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     1021 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     1081 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     1141 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     1201 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     1261 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     1321 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     1381 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     1441 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     1501 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1561 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1621 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1681 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1741 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1801 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1861 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1921 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1981 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2041 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2101 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2161 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2221 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     2281 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     2341 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     2401 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatagac
     2461 cagccgcgta acctggcaaa atcggttacg gttgagtaat aaatggatgc cctgcgtaag
     2521 cgggtgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac tatgacaata
     2581 aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg ctgtgaaaaa
     2641 gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt gcaaattgcc
     2701 cgtcgtatta aagaggggcg tggccaaggg catggtaaag actatattcg cggcgttgtg
     2761 acaatttacc gaacaactcc gcggccggga agccgatctc ggcttgaacg aattgttagg
     2821 tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat
     2881 agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa
     2941 gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt
     3001 gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg
     3061 gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga
     3121 tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt
     3181 aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga
     3241 cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg
     3301 ttttagggcg actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac
     3361 ccacggcgta acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt
     3421 cataacaagc catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc
     3481 tggaccagtt gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt
     3541 atgtcaactg ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg
     3601 gcagcagcga agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct
     3661 ccacgcatcg tcaggcattg gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg
     3721 atctgccctg gcttcaggag atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc
     3781 tgaccccgga tgaagtggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg
     3841 cccaggactc tagctatagt tctagtggtt ggctacgtat actccggaat attaatagat
     3901 catggagata attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt
     3961 aacagttttg taataaaaaa acctataaat attccggatt attcataccg tcccaccatc
     4021 gggcgcggat cccggtccga agcgcgcgga attcaaaggc ctacgtcgac gccatgagcg
     4081 atcgcatgcg cgcgcacccg gggggaggcc gctgctgccc ggagcaggag gagggggaga
     4141 gcgcggcggg cggcagcggc gctggcggcg actccgccat agagcagggg ggccagggca
     4201 gcgcgctcgc tccgtccccg gtgagcggcg tgcgcaggga aggcgctcgg ggcggcggcc
     4261 gtggccgggg gcggtggaag caggcggccc ggggcggcgg cgtctgtggc cgtggccgtg
     4321 gccgtggccg gggtcggggc cgtggccggg gccggggccg gggccgcggc cgtccccaga
     4381 gtggcggcag cggccttggc ggcgacggcg gcggcggcgc gggcggctgc ggcgtcggca
     4441 gcggtggcgg cgtcgccccc cggcgggatc ctgtcccttt cccgtcgggg agctcggggc
     4501 cggggcccag gggaccccgg gccacggaga gcgggaagag ggcggcccgg gatccaccgg
     4561 tcgacatggc ctcctccgag gacgtcatca aggagttcat gcgcttcaag gtgcgcatgg
     4621 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     4681 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     4741 acatcctgtc ccctcagttc cagtacggct ccaaggccta cgtgaagcac cccgccgaca
     4801 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     4861 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     4921 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     4981 agaccatggg ctgggaggcc tccaccgagc ggatgtaccc cgaggacggc gccctgaagg
     5041 gcgagatcaa gatgaggctg aagctgaagg acggcggcca ctacgacgcc gaggtcaaga
     5101 ccacctacat ggccaagaag cccgtgcagc tgcccggcgc ctacaagacc gacatcaagc
     5161 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgcgccgagg
     5221 gccgccactc caccggcgcc ggcctgtaca agtaaagcgg ccgctttcga atctagagcc
     5281 tgcagtctcg aggcatgcgg taccaagctt gtcgagaagt actagaggat cataatcagc
     5341 cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     5401 ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     5461 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     5521 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt
     5581 gagcctagga gatccgaacc agataagtga aatctagttc caaactattt tgtcattttt
     5641 aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct
     5701 aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca
     5761 cagcggggca tttttcttcc tgttatgttt ttaatcaaac atcctgccaa ctccatgtga
     5821 caaaccgtca tcttcggcta ctttttctct gtcacagaat gaaaattttt ctgtcatctc
     5881 ttcgttatta atgtttgtaa ttgactgaat atcaacgctt atttgcagcc
//
