LOCUS       sequence-422749-        5616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This binary vector expresses NanoLuciferase (Nluc) with the 
            truncated 35sCAMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5616)
  TITLE     Curtis lab plasmids
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLSU1/t35s[]:NLuc:NosT
FEATURES             Location/Qualifiers
     source          1..5616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..195
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     658..678
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        667..712
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      1250..1502
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1678..1697)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1689..2504
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1689..2504
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1762..1781)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2590..3178
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3079..3098
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3420..4049
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4478..5551
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatgatat cgcatgcgga aacctcctcg gattccattg
      421 cccagctatc tgtcacttta ttgagaagat agtggaaaag gaaggtggct cctacaaatg
      481 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
      541 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      601 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
      661 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggcctacgcg
      721 tatggtgttt actcttgaag atttcgtggg agactggcgt caaacagcag gctataactt
      781 agaccaggtc ctagaacagg gaggcgtttc cagtctgttt cagaatcttg gagttagtgt
      841 tacacctatc caaagaatag ttctcagcgg tgaaaatggc ctcaaaatag acatccatgt
      901 gatcatccca tacgagggct tgtccggcga tcaaatgggc cagatagaga aaatattcaa
      961 agtagtgtac ccagtagatg accaccactt taaggtaata cttcactacg ggaccttagt
     1021 tatagacggg gtaaccccta atatgatcga ctacttcggc cgtccctacg agggcattgc
     1081 tgtcttcgat gggaagaaga taacagttac cggcactttg tggaacggta acaaaatcat
     1141 cgacgagcga ctgatcaacc cagatggtag ccttctgttc agggttacca ttaacggggt
     1201 tacagggtgg cgtctgtgcg agcgtatatt agcttaagta tacctgcagg atcgttcaaa
     1261 catttggcaa taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat
     1321 ataatttctg ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt
     1381 tatgagatgg gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa
     1441 caaaatatag cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga
     1501 tcggggagct ctggcaggat atatgcggtt gtaattcatt tttattgtct aaatttctgt
     1561 atttgtttgt ttgcaattag atccacgttg tgtctcaaaa tctctgatgt tacattgcac
     1621 aagataaaaa tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa
     1681 ggggtgttat gagccatatt caacgggaaa cgtcttgttc gaggccgcga ttaaattcca
     1741 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     1801 cgacaatcta ccgattgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     1861 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     1921 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     1981 ccactgcgat cccagggaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     2041 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     2101 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     2161 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     2221 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     2281 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     2341 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     2401 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     2461 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     2521 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcggcttt gttgaatcaa
     2581 ttgatcttct tgaaatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2641 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2701 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     2761 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2821 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2881 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2941 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3001 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3061 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3121 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3181 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3241 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgatctct
     3301 gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt
     3361 catgcggtcg ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca
     3421 tgaaggttat cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc
     3481 atctagcccg cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc
     3541 agggcagtgc ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca
     3601 tcgaccgccc gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga
     3661 tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg
     3721 tgctgattcc ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc
     3781 tggttaagca gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc
     3841 gggcgatcaa aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc
     3901 tgcccattct tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg
     3961 gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg
     4021 ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag
     4081 cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc
     4141 cagcctggca gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca
     4201 caccaagctg aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga
     4261 atacatcgcg cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag
     4321 cggctaaagg aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc
     4381 ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct
     4441 gcaatggcac tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg
     4501 cccggtacaa atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca
     4561 ggccgcccag cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc
     4621 cgctgatcga atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag
     4681 gaagccgccc aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg
     4741 cacccgcgat agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg
     4801 acgagctggc gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg
     4861 gccggccggc atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct
     4921 aaccgaatcc atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg
     4981 tccacacgtt gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga
     5041 cgacctggta gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa
     5101 gaaggccaag aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta
     5161 caagatcgta aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagttgattg
     5221 gatgtaccgc gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta
     5281 ctttttgatc gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg
     5341 caaggcagaa gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga
     5401 gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta
     5461 cgatttgaag gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct
     5521 gatcgagggc gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc
     5581 cctagcaggg gaaaaaggtc gaaaagatct ctttcc
//
