LOCUS       sequence-422786-        5702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MeCP2 (aa1-76) GFP tagged in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Becker A, Allmann L, Hofstatter M, Casa V, Weber P, Lehmkuhl A, 
            Herce HD, Cardoso MC
  TITLE     Direct homo- and hetero-interactions of MeCP2 and MBD2.
  JOURNAL   PLoS One. 2013;8(1):e53730. doi: 10.1371/journal.pone.0053730. Epub 
            2013 Jan 15.
  PUBMED    23335972
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e53730. doi"; pages: " 
            10.1371/journal.pone.0053730. Epub 2013 Jan 15"
COMMENT     Sequence Label: pFBMeCP2G.10 (pc1577)
FEATURES             Location/Qualifiers
     source          1..5702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      9..463
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(96..115)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     305..326
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        489..593
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(594..662,663..1454)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(812..831)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1625..2213
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2114..2133
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2367..2384
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2518..2742
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2809..3342)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3512..3517,3518..3534,3535..3540))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3892..3983
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3908..3925
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(4317..4319,4320..4322,4323..5036)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4317..4319,4320..4322,4323..5033)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4362..4383)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4623..4642)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4970..4991
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5199..5333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5236..5255)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5290..5309
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5362..5527
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
       61 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      121 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
      181 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
      241 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
      301 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
      361 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
      421 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
      481 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      541 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
      601 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
      661 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
      721 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
      781 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
      841 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
      901 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
      961 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1021 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1081 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1141 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1201 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1261 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1321 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     1381 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     1441 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     1501 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     1561 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1621 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1681 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1741 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     1801 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1861 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1921 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1981 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2041 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2101 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2161 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2221 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2281 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2341 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2401 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata gaccagccgc
     2461 gtaacctggc aaaatcggtt acggttgagt aataaatgga tgccctgcgt aagcgggtgt
     2521 gggcggacaa taaagtctta aactgaacaa aatagatcta aactatgaca ataaagtctt
     2581 aaactagaca gaatagttgt aaactgaaat cagtccagtt atgctgtgaa aaagcatact
     2641 ggacttttgt tatggctaaa gcaaactctt cattttctga agtgcaaatt gcccgtcgta
     2701 ttaaagaggg gcgtggccaa gggcatggta aagactatat tcgcggcgtt gtgacaattt
     2761 accgaacaac tccgcggccg ggaagccgat ctcggcttga acgaattgtt aggtggcggt
     2821 acttgggtcg atatcaaagt gcatcacttc ttcccgtatg cccaactttg tatagagagc
     2881 cactgcggga tcgtcaccgt aatctgcttg cacgtagatc acataagcac caagcgcgtt
     2941 ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc
     3001 ggagactgcg agatcataga tatagatctc actacgcggc tgctcaaact tgggcagaac
     3061 gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt
     3121 cttactacgg agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc
     3181 tacgtctccg aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc
     3241 agggccgagc ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg
     3301 gcgactgccc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
     3361 gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca
     3421 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
     3481 gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa
     3541 ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag
     3601 cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca
     3661 tcgtcaggca ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc
     3721 ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc
     3781 ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagga
     3841 ctctagctat agttctagtg gttggctacg tatactccgg aatattaata gatcatggag
     3901 ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt
     3961 ttgtaataaa aaaacctata aatattccgg attattcata ccgtcccacc atcgggcgcg
     4021 gatcccggtc cgaagcgcgc ggaattcaaa ggcctacgtc gacaagcgat cgcgccatgg
     4081 tagctgggat gttagggctc aggaaggaaa agtcagaaga ccaggatctc cagggcctca
     4141 aagagaaacc cctgaagttt aagaaggtga agaaagacaa gaaggaagac aaagagggca
     4201 aacatgaacc actacagcct tcagcccacc attctgcaga gccagcagag gcaggcaaag
     4261 cagagacatc agaaagctca ggctctgccc cagcagtacc agaaagcggc cgcgccatgg
     4321 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     4381 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     4441 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     4501 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     4561 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     4621 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     4681 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     4741 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     4801 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     4861 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     4921 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     4981 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaatcta
     5041 gagcctgcag tctcgaggca tgcggtacca agcttgtcga gaagtactag aggatcataa
     5101 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5161 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac
     5341 tgcttgagcc taggagatcc gaaccagata agtgaaatct agttccaaac tattttgtca
     5401 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
     5461 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
     5521 gcccacagcg gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca
     5581 tgtgacaaac cgtcatcttc ggctactttt tctctgtcac agaatgaaaa tttttctgtc
     5641 atctcttcgt tattaatgtt tgtaattgac tgaatatcaa cgcttatttg cagcctgaat
     5701 gg
//
