LOCUS       sequence-422787-        6076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses MBD2 (aa222-414) mCherry tagged in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6076)
  AUTHORS   Becker A, Allmann L, Hofstatter M, Casa V, Weber P, Lehmkuhl A, 
            Herce HD, Cardoso MC
  TITLE     Direct homo- and hetero-interactions of MeCP2 and MBD2.
  JOURNAL   PLoS One. 2013;8(1):e53730. doi: 10.1371/journal.pone.0053730. Epub 
            2013 Jan 15.
  PUBMED    23335972
REFERENCE   2  (bases 1 to 6076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e53730. doi"; pages: " 
            10.1371/journal.pone.0053730. Epub 2013 Jan 15"
COMMENT     Sequence Label: pFB-MBD2.3Ch (pc2392)
FEATURES             Location/Qualifiers
     source          1..6076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..460
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(93..112)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     302..323
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        486..590
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(591..659,660..1451)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(809..828)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1622..2210
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2111..2130
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2364..2381
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2515..2739
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2806..3339)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3509..3514,3515..3531,3532..3537))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3889..3980
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3905..3922
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             4658..4678
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4685..5395
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4830..4848)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5098..5117
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    5570..5704
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5607..5626)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5661..5680
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5733..5898
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
       61 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      121 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      181 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      241 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttcttaat
      301 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
      361 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
      421 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
      481 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      541 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      601 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      661 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      721 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
      781 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
      841 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
      901 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
      961 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     1021 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     1081 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     1141 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     1201 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     1261 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     1321 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1381 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1441 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1501 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1561 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1621 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1681 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1741 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1801 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1861 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1921 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1981 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     2041 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     2101 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     2161 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     2221 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     2281 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     2341 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga
     2401 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatagac cagccgcgta
     2461 acctggcaaa atcggttacg gttgagtaat aaatggatgc cctgcgtaag cgggtgtggg
     2521 cggacaataa agtcttaaac tgaacaaaat agatctaaac tatgacaata aagtcttaaa
     2581 ctagacagaa tagttgtaaa ctgaaatcag tccagttatg ctgtgaaaaa gcatactgga
     2641 cttttgttat ggctaaagca aactcttcat tttctgaagt gcaaattgcc cgtcgtatta
     2701 aagaggggcg tggccaaggg catggtaaag actatattcg cggcgttgtg acaatttacc
     2761 gaacaactcc gcggccggga agccgatctc ggcttgaacg aattgttagg tggcggtact
     2821 tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc aactttgtat agagagccac
     2881 tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca taagcaccaa gcgcgttggc
     2941 ctcatgcttg aggagattga tgagcgcggt ggcaatgccc tgcctccggt gctcgccgga
     3001 gactgcgaga tcatagatat agatctcact acgcggctgc tcaaacttgg gcagaacgta
     3061 agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca gcaagcgcga tgaatgtctt
     3121 actacggagc aagttcccga ggtaatcgga gtccggctga tgttgggagt aggtggctac
     3181 gtctccgaac tcacgaccga aaagatcaag agcagcccgc atggatttga cttggtcagg
     3241 gccgagccta catgtgcgaa tgatgcccat acttgagcca cctaactttg ttttagggcg
     3301 actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
     3361 acgcgcttgc tgcttggatg cccgaggcat agactgtaca aaaaaacagt cataacaagc
     3421 catgaaaacc gccactgcgc cgttaccacc gctgcgttcg gtcaaggttc tggaccagtt
     3481 gcgtgagcgc atacgctact tgcattacag tttacgaacc gaacaggctt atgtcaactg
     3541 ggttcgtgcc ttcatccgtt tccacggtgt gcgtcacccg gcaaccttgg gcagcagcga
     3601 agtcgaggca tttctgtcct ggctggcgaa cgagcgcaag gtttcggtct ccacgcatcg
     3661 tcaggcattg gcggccttgc tgttcttcta cggcaaggtg ctgtgcacgg atctgccctg
     3721 gcttcaggag atcggaagac ctcggccgtc gcggcgcttg ccggtggtgc tgaccccgga
     3781 tgaagtggtt cgcatcctcg gttttctgga aggcgagcat cgtttgttcg cccaggactc
     3841 tagctatagt tctagtggtt ggctacgtat actccggaat attaatagat catggagata
     3901 attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt aacagttttg
     3961 taataaaaaa acctataaat attccggatt attcataccg tcccaccatc gggcgcggat
     4021 cccggtccga agcgcgcgga attcaaaggc ctacgtcgac atgcagaaga acaagcagag
     4081 actccggaat gaccccctca atcagaacaa gggtaaacca gacctgaaca caacattgcc
     4141 aattagacaa actgcatcaa ttttcaagca accagtaacc aaattcacga accacccgag
     4201 caataaggtg aagtcagacc cccagcggat gaatgaacaa ccacgtcagc ttttctggga
     4261 gaagaggcta caaggactta gcgcatcaga tgtaacagaa caaattataa aaaccatgga
     4321 gctacctaaa ggtcttcaag gagtcggtcc aggtagcaat gacgagaccc ttctgtctgc
     4381 tgtggccagt gctttacaca caagctctgc gcccatcaca ggacaagtct ctgctgccgt
     4441 ggaaaagaac cctgctgttt ggcttaacac atctcaaccc ctctgcaaag ctttcattgt
     4501 tacagatgaa gacattagga aacaggaaga gcgagtccaa caagtacgca agaaactgga
     4561 ggaggcactg atggccgaca tcctgtcccg ggctgcggac acggaggaag tagacattga
     4621 catggacgat ggagatgagg cgtgcggccc gggatccccg aagaagaagc gcaaagtata
     4681 caccatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
     4741 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     4801 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
     4861 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     4921 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     4981 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     5041 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     5101 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     5161 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     5221 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     5281 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     5341 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtaaagcgg
     5401 ccgcgactct agagcctgca gtctcgaggc atgcggtacc aagcttgtcg agaagtacta
     5461 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     5521 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     5581 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     5641 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     5701 gatctgatca ctgcttgagc ctaggagatc cgaaccagat aagtgaaatc tagttccaaa
     5761 ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat
     5821 tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa
     5881 ctattttgtc cgcccacagc ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc
     5941 tgccaactcc atgtgacaaa ccgtcatctt cggctacttt ttctctgtca cagaatgaaa
     6001 atttttctgt catctcttcg ttattaatgt ttgtaattga ctgaatatca acgcttattt
     6061 gcagcctgaa tggcga
//
