LOCUS       sequence-422821-        5784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This binary vector expresses mCherry with the truncated 35sCAMV 
            promoter and no UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5784)
  TITLE     Curtis lab plasmids
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLSU1/t35s[]:mCherry:NosT
FEATURES             Location/Qualifiers
     source          1..5784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..195
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     658..678
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        667..712
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      1418..1670
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1846..1865)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1857..2672
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1857..2672
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1930..1949)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2758..3346
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3247..3266
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3588..4217
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4646..5719
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatgatat cgcatgcgga aacctcctcg gattccattg
      421 cccagctatc tgtcacttta ttgagaagat agtggaaaag gaaggtggct cctacaaatg
      481 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
      541 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      601 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
      661 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggcctacgcg
      721 tatggccatc atcaaggagt tcatgcggtt caaggtgcac atggaaggca gcgtgaacgg
      781 ccacgagttc gagatcgagg gcgagggcga aggcaggccc tacgagggca cccagaccgc
      841 caagctgaag gtgaccaagg gcggacctct gcccttcgcc tgggacatcc tgagccccca
      901 gttcatgtac ggcagcaagg cctacgtgaa gcaccccgcc gacatccccg actacctgaa
      961 gctgtccttc cccgagggat tcaaatggga gcgggtgatg aacttcgagg acggcggcgt
     1021 ggtgaccgtg acccaggaca gcagcctgca ggacggcgag ttcatctaca aggtgaaact
     1081 gcggggcacc aacttcccca gcgacggccc cgtgatgcag aaaaagacca tgggctggga
     1141 ggccagcagc gagcggatgt accccgagga tggcgccctg aagggcgaga tcaagcagcg
     1201 gctgaagctg aaggatggcg gccactacga cgccgaggtg aaaaccacct acaaggccaa
     1261 gaaacccgtg cagctgcctg gcgcctacaa cgtgaacatc aagctggaca tcaccagcca
     1321 caacgaggac tacaccatcg tggagcagta cgagcgggcc gagggcagac acagcaccgg
     1381 cggcatggac gagctgtaca agtaggtata cctgcaggat cgttcaaaca tttggcaata
     1441 aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat aatttctgtt
     1501 gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta tgagatgggt
     1561 ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca aaatatagcg
     1621 cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc ggggagctct
     1681 ggcaggatat atgcggttgt aattcatttt tattgtctaa atttctgtat ttgtttgttt
     1741 gcaattagat ccacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     1801 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
     1861 gccatattca acgggaaacg tcttgttcga ggccgcgatt aaattccaac atggatgctg
     1921 atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctacc
     1981 gattgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg
     2041 ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc
     2101 cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc
     2161 cagggaaaac agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg
     2221 atgcgctggc agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta
     2281 acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg
     2341 atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa
     2401 tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg
     2461 ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa
     2521 tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt
     2581 cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc
     2641 agtttcattt gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact
     2701 ggcagagcat tacgctgact tgacgggacg gcggctttgt tgaatcaatt gatcttcttg
     2761 aaatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2821 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2881 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2941 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3001 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3061 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3121 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3181 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3241 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3301 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     3361 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     3421 atcccctgat tctgtggata accgtattac cgcctttgag tgatctctga agaaaccgag
     3481 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct
     3541 gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg
     3601 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg
     3661 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc
     3721 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga
     3781 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc
     3841 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg
     3901 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc
     3961 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag
     4021 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg
     4081 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc
     4141 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta
     4201 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct
     4261 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga
     4321 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa
     4381 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca
     4441 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag
     4501 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag
     4561 gaacgggcgg ttggccaggc gtaagcggct gggttgtctg ccggccctgc aatggcactg
     4621 gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat
     4681 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg
     4741 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat
     4801 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa
     4861 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag
     4921 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga
     4981 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat
     5041 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat
     5101 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc
     5161 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga
     5221 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa
     5281 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa
     5341 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gttgattgga tgtaccgcga
     5401 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga
     5461 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc
     5521 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt
     5581 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga
     5641 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga
     5701 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga
     5761 aaaaggtcga aaagatctct ttcc
//
