LOCUS       sequence-422827-        6380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This binary vector expresses mNeonGreen with the original 35sCAMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6380)
  TITLE     Curtis lab plasmids
REFERENCE   2  (bases 1 to 6380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLSU1/o35S[]:mNeonGreen:NosT
FEATURES             Location/Qualifiers
     source          1..6380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..195
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     promoter        876..1221
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1164..1184
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     terminator      1963..2215
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2442..2461)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2453..3268
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2453..3268
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2526..2545)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3354..3942
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3843..3862
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             4184..4813
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             5242..6315
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatgatag attagccttt tcaatttcag aaagaatgct
      421 aacccacaga tggttagaga ggcttacgca gcaggtctca tcaagacgat ctacccgagc
      481 aataatctcc aggaaatcaa ataccttccc aagaaggtta aagatgcagt caaaagattc
      541 aggactaact gcatcaagaa cacagagaaa gatatatttc tcaagatcag aagtactatt
      601 ccagtatgga cgattcaagg cttgcttcac aaaccaaggc aagtaataga gattggagtc
      661 tctaaaaagg tagttcccac tgaatcaaag gccatggagt caaagattca aatagaggac
      721 ctaacagaac tcgccgtaaa gactggcgaa cagttcatac agagtctctt acgactcaat
      781 gacaagaaga aaatcttcgt caacatggtg gagcacgaca cacttgtcta ctccaaaaat
      841 atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca aagggtaata
      901 tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt gaagatagtg
      961 gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc catcgttgaa
     1021 gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa
     1081 aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgatat ctccactgac
     1141 gtaagggatg acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt
     1201 tcatttcatt tggagagaac acgggggact ctagaatggt ttccaaaggt gaggaagata
     1261 atatggctag tttacctgcc acacatgagc tacacatatt cggcagcata aacggtgtag
     1321 attttgatat ggttggacaa gggacaggta atcctaatga tggatatgag gagttgaacc
     1381 tcaaatctac caagggggac cttcaattct ccccatggat cctagtccct cacattggat
     1441 acggcttcca ccagtactta ccatacccag atggaatgtc ccctttccaa gcagccatgg
     1501 ttgacggttc cgggtaccag gtacatagga caatgcaatt tgaggacggg gcttctctga
     1561 ctgtgaatta ccgatatacc tatgaaggca gtcacataaa gggcgaagca caagtgaagg
     1621 gcaccggatt tcctgccgac gggcctgtaa tgactaatag cttgaccgcc gcagattggt
     1681 gcaggtcaaa aaagacctac ccaaatgaca agacaataat ctctacattt aagtggagct
     1741 acactacagg gaatggtaaa cgatatagat caactgctag aaccacatat accttcgcta
     1801 aacctatggc agccaattat ctgaagaacc agcccatgta tgtgttccga aagactgaac
     1861 tcaagcatag caaaaccgaa ctcaacttca aagagtggca aaaggctttc actgacgtga
     1921 tgggaatgga cgagttatat aagtaagagc tcgaatttcc ccgatcgttc aaacatttgg
     1981 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     2041 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     2101 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     2161 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcaagct
     2221 tccatggctc gagggtaccg aattcggatc cctgcagagt acttctagag agctctggca
     2281 ggatatatgc ggttgtaatt catttttatt gtctaaattt ctgtatttgt ttgtttgcaa
     2341 ttagatccac gttgtgtctc aaaatctctg atgttacatt gcacaagata aaaatatatc
     2401 atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg ttatgagcca
     2461 tattcaacgg gaaacgtctt gttcgaggcc gcgattaaat tccaacatgg atgctgattt
     2521 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctaccgatt
     2581 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     2641 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     2701 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatcccagg
     2761 gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     2821 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     2881 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     2941 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     3001 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     3061 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     3121 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     3181 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     3241 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     3301 gagcattacg ctgacttgac gggacggcgg ctttgttgaa tcaattgatc ttcttgaaat
     3361 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3421 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3481 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3541 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3601 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3661 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3721 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3781 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3841 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3901 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3961 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     4021 cctgattctg tggataaccg tattaccgcc tttgagtgat ctctgaagaa accgagcgcc
     4081 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
     4141 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
     4201 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
     4261 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
     4321 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
     4381 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
     4441 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
     4501 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
     4561 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
     4621 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
     4681 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
     4741 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     4801 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     4861 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     4921 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     4981 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
     5041 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
     5101 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
     5161 gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac
     5221 ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc
     5281 gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa
     5341 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc
     5401 aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc
     5461 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc
     5521 agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg
     5581 atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc
     5641 agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac
     5701 cgataccggg aagggaaggg agacaagccc ggccgcgtgt tccgtccaca cgttgcggac
     5761 gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc
     5821 tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc
     5881 cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc
     5941 gaaaccgggc ggccggagta catcgagatc gagctagttg attggatgta ccgcgagatc
     6001 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc
     6061 ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga
     6121 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt
     6181 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag
     6241 gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca
     6301 tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa
     6361 ggtcgaaaag atctctttcc
//
