LOCUS       sequence-422848-        2639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses untagged ATG3 (H266A) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Rao S, Skulsuppaisarn M, Strong LM, Ren X, Lazarou M, Hurley JH, 
            Hummer G
  TITLE     Three-step docking by WIPI2, ATG16L1, and ATG3 delivers LC3 to the 
            phagophore.
  JOURNAL   Sci Adv. 2024 Feb 9;10(6):eadj8027. doi: 10.1126/sciadv.adj8027. 
            Epub 2024 Feb 7.
  PUBMED    38324698
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2024-02-9"; volume: "10(6):eadj8027. doi"; pages: " 
            10.1126/sciadv.adj8027. Epub 2024 Feb 7"
COMMENT     Sequence Label: pMX-IG-ATG3 (H266A)
FEATURES             Location/Qualifiers
     source          1..2639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        142..246
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(247..315,316..1107)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(465..484)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1271..1859
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1760..1779
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2013..2030
                     /label=L4440
                     /note="L4440 vector, forward primer"
     LTR             2160..2629
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 tgttcctgac cttgatctga acttttctat tctcagttat gtatttttcc atgccttgca
       61 aaatggcgtt acttaagcta gcttgccaaa cctacaggtg gggtctttca ttatgcaggt
      121 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
      181 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
      241 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
      301 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
      361 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
      421 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
      481 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
      541 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
      601 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
      661 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
      721 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
      781 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
      841 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
      901 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
      961 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     1021 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     1081 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     1141 attgatttgc ggccggccgc aaaaggatct aggtgaagat cctttttgat aatctcatga
     1201 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     1261 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     1321 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     1381 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     1441 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     1501 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     1561 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1621 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     1681 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     1741 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     1801 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     1861 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     1921 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     1981 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     2041 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactatg
     2101 gccatttaat gtaaatactt aagaaaaaaa accaaattaa ttttgataca tgctgcatgt
     2161 gaaagacccc cgctgacggg tagtcaatca ctcagaggag accctcccaa ggaacagcga
     2221 gaccacaagt cggatgcaac tgcaagaggg tttattggat acacgggtac ccgggcgact
     2281 cagtcaatcg gaggactggc gccccgagtg aggggttgtg ggctctttta ttgagctcgg
     2341 ggagcagaag cgcgcgaaca gaagcgagaa gcgaactgat tggttagttc aaataaggca
     2401 cagggtcatt tcaggtcctt ggggcaccct ggaaacatct gatggttctc tagaaactgc
     2461 tgagggctgg accgcatctg gggaccatct gttcttggcc ctgagccggg gcaggaactg
     2521 cttaccacag atatcctgtt tggcccatat tcagctgttc catctgttct tggccctgag
     2581 ccggggcagg aactgcttac cacagatatc ctgtttggcc catattcagc tgttccatc
//
