LOCUS       sequence-422856-        5873 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This binary vector expresses mCherry with the truncated 35sCAMV 
            promoter and Tomato Mosaic Virus UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5873)
  TITLE     Curtis lab plasmids
REFERENCE   2  (bases 1 to 5873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLSU1/t35s[TMV]:mCherry:NosT
FEATURES             Location/Qualifiers
     source          1..5873
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..195
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     658..678
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        667..713
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     terminator      1507..1759
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(1935..1954)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1946..2761
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             1946..2761
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2019..2038)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2847..3435
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3336..3355
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3677..4306
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4735..5808
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
       61 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
      121 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
      181 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
      241 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
      301 ccgctcaaaa atggctggcc tacggccagg caatctgcta gcgcgcggac aagctaggat
      361 tggcaggata tattcagttg taaatgatat cgcatgcgga aacctcctcg gattccattg
      421 cccagctatc tgtcacttta ttgagaagat agtggaaaag gaaggtggct cctacaaatg
      481 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
      541 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      601 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
      661 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctc
      721 gagtataaga gctcattttt acaacaatta ccaacaacaa caaacaacaa acaacattac
      781 aattacattt acaattatcg atacacgcgt atggccatca tcaaggagtt catgcggttc
      841 aaggtgcaca tggaaggcag cgtgaacggc cacgagttcg agatcgaggg cgagggcgaa
      901 ggcaggccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg cggacctctg
      961 cccttcgcct gggacatcct gagcccccag ttcatgtacg gcagcaaggc ctacgtgaag
     1021 caccccgccg acatccccga ctacctgaag ctgtccttcc ccgagggatt caaatgggag
     1081 cgggtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggacag cagcctgcag
     1141 gacggcgagt tcatctacaa ggtgaaactg cggggcacca acttccccag cgacggcccc
     1201 gtgatgcaga aaaagaccat gggctgggag gccagcagcg agcggatgta ccccgaggat
     1261 ggcgccctga agggcgagat caagcagcgg ctgaagctga aggatggcgg ccactacgac
     1321 gccgaggtga aaaccaccta caaggccaag aaacccgtgc agctgcctgg cgcctacaac
     1381 gtgaacatca agctggacat caccagccac aacgaggact acaccatcgt ggagcagtac
     1441 gagcgggccg agggcagaca cagcaccggc ggcatggacg agctgtacaa gtaggtatac
     1501 ctgcaggatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     1561 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     1621 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     1681 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     1741 tcatctatgt tactagatcg gggagctctg gcaggatata tgcggttgta attcattttt
     1801 attgtctaaa tttctgtatt tgtttgtttg caattagatc cacgttgtgt ctcaaaatct
     1861 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     1921 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgttcgag
     1981 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     2041 tgtcgggcaa tcaggtgcga caatctaccg attgtatggg aagcccgatg cgccagagtt
     2101 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     2161 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     2221 tgatgcatgg ttactcacca ctgcgatccc agggaaaaca gcattccagg tattagaaga
     2281 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     2341 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     2401 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     2461 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     2521 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     2581 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     2641 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     2701 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     2761 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     2821 cggctttgtt gaatcaattg atcttcttga aatccttttt ttctgcgcgt aatctgctgc
     2881 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2941 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3001 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3061 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3121 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3181 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3241 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3301 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     3361 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     3421 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     3481 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     3541 gcctttgagt gatctctgaa gaaaccgagc gccgccgtct aaaaaggtga tgtgtatttg
     3601 agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta aataaacaaa
     3661 tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg ggtcaggcaa
     3721 gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg atgttctgtt
     3781 agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg aagatcaacc
     3841 gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg ccatcggccg
     3901 gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg tgtccgcgat
     3961 caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca tatgggccac
     4021 cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa ggctacaagc
     4081 ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg ttgccgaggc
     4141 gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg tgagctaccc
     4201 aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg acgctgcccg
     4261 cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta atgaggtaaa
     4321 gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc acgcagcagc
     4381 aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt caactttcag
     4441 ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg caagaccatt
     4501 accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa atgaataaat
     4561 gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac aaccaggcac
     4621 cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg taagcggctg
     4681 ggttgtctgc cggccctgca atggcactgg aacccccaag cccgaggaat cggcgtgagc
     4741 ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga cctggtggag
     4801 aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc acgccccggt
     4861 gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc gccggcagcc
     4921 ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt tttcgttccg
     4981 atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc cgttttccgt
     5041 ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc agacgggcac
     5101 gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga cctggtactg
     5161 atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa gggagacaag
     5221 cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg gcgagccgat
     5281 ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac cacgcacgtt
     5341 gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc cgagggtgaa
     5401 gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga gtacatcgag
     5461 atcgagctag ttgattggat gtaccgcgag atcacagaag gcaagaaccc ggacgtgctg
     5521 acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct ctaccgcctg
     5581 gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat ctacgaacgc
     5641 agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct gatcgggtca
     5701 aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc gatcctagtc
     5761 atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg tacggagcag
     5821 atgctagggc aaattgccct agcaggggaa aaaggtcgaa aagatctctt tcc
//
