LOCUS       sequence-42287-e         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  TITLE     
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MCTS1
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(83..100)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    690..714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(714..733)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(715..733)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa gcttgtcgac
       61 ggagctcgaa ttcggatcct caatggtggt gatgatggtg cgctttatat gtcttcatat
      121 gccacagccc atcatttaaa taatggatat tttcaatgcc aattcctttg ttgactttct
      181 caatgtcttc tgcagacatc ttcatgactc caacacatag agcatgctgt tttccttctg
      241 ccatgatagc aacaatggta tctactgcag cagggtaaag cttagctcca ggagaagtta
      301 agcctggaca catgatattt gctccactga gtacaaattt gatggctcct ttatcaacct
      361 gctggtgtgg caggataaaa ggatatttgt gaagtaatct tagggttgga taaaaaggcc
      421 cttctctttg tctaaaaaag agtaattctc catttactgt aaggatttct atatgttcat
      481 ggcatcggac tattttgaca ggatctttct taggcatgat ttgattaagc catggttcaa
      541 tacctggaaa ttgctctatc aattgattct taataccctt aataactgaa gttttcaact
      601 ggatgcagtt ggacacattt tctttttcat caaatttctt gaacatagta tatctccttc
      661 ttaaggttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata
      721 gtgagtcgta ttaatttcgc gggatcgaga tctcgatcct ctac
//
