LOCUS       sequence-422874-        8738 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A single vector containing a CAG-driven Cas9 variant from S. 
            thermophilus recognizing a consensus NNACAA PAM (St1Cas9 CNRZ1066 
            v2) and its U6-driven sgRNA targeting human ATM.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8738)
  TITLE     Non-canonical regulatory role of KAT5 chromodomain, independent of 
            histone marks, in NuA4/TIP60 function on chromatin
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: U6_ ATM_G101_sgRNA_CAG_St1Cas9_CNRZ1066_v2
FEATURES             Location/Qualifiers
     source          1..8738
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        94..342
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     94..114
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     265..284
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        514..817
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1095..2111
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2035..2057
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2119..2138
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      2204..2213
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2219..2239
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2264..5623
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus 
                     thermophilus Type II CRISPR/Cas system (Esvelt et al., 
                     2013)"
                     /label=St1Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="SDLVLGLDIGIGSVGVGILNKVTGEIIHKNSRIFPAAQAENNLVR
                     RTNRQGRRLARRKKHRRVRLNRLFEESGLITDFTKISINLNPYQLRVKGLTDELSNEEL
                     FIALKNMVKHRGISYLDDASDDGNSSVGDYAQIVKENSKQLETKTPGQIQLERYQTYGQ
                     LRGDFTVEKDGKKHRLINVFPTSAYRSEALRILQTQQEFNPQITDEFINRYLEILTGKR
                     KYYHGPGNEKSRTDYGRYRTSGETLDNIFGILIGKCTFYPDEFRAAKASYTAQEFNLLN
                     DLNNLTVPTETKKLSKEQKNQIINYVKNEKAMGPAKLFKYIAKLLSCDVADIKGYRIDK
                     SGKAEIHTFEAYRKMKTLETLDIEQMDRETLDKLAYVLTLNTEREGIQEALEHEFADGS
                     FSQKQVDELVQFRKANSSIFGKGWHNFSVKLMMELIPELYETSEEQMTILTRLGKQKTT
                     SSSNKTKYIDEKLLTEEIYNPVVAKSVRQAIKIVNAAIKEYGDFDNIVIEMARETNEDD
                     EKKAIQKIQKANKDEKDAAMLKAANQYNGKAELPHSVFHGHKQLATKIRLWHQQGERCL
                     YTGKTISIHDLINNSNQFEVDHILPLSITFDDSLANKVLVYATANQEKGQRTPYQALDS
                     MDDAWSFRELKAFVRESKTLSNKKKEYLLTEEDISKFDVRKKFIERNLVDTRYASRVVL
                     NALQEHFRAHKIDTKVSVVRGQFTSQLRRHWGIEKTRDTYHHHAVDALIIAASSQLNLW
                     KKQKNTLVSYSEDQLLDIETGELISDDEYKESVFKAPYQHFVDTLKSKEFEDSILFSYQ
                     VDSKFNRKISDATIYATRQAKVGKDKADETYVLGKIKDIYTQDGYDAFMKIYKKDKSKF
                     LMYRHDPQTFEKVIEPILENYPNKQINEKGKEVPCNPFLKYKEEHGYIRKYSKKGNGPE
                     IKSLKYYDSKLGNHIDITPKDSNNKVVLQSLKPWRTDVYFNKATGKYEILGLKYADLQF
                     EKGTGTYKISQEKYNDIKKKEGVDSDSEFKFTLYKNDLLLVKDTETKEQQLFRFLSRTL
                     PKQKHYVELKPYDKQKFEGGEALIKVLGNVANGGQCIKGLAKSNISIYKVRTDVLGNQH
                     IIKNEGDKPKLDF"
     CDS             5636..5656
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(5760..5779)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5825..5880
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5879..5898)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6241..6257)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6241..6257)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6254..6276)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6265..6281
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6289..6295,6296..6313,6314..6319))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6334..6355
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6414..6609
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6460..6595
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6615..6749
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6652..6671)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6706..6725
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6817..6834)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6988..7576)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7068..7087)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7747..8538,8539..8607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8370..8389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8608..8712)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagctgtaca aaaaagcagg ctttaaagga accaattcag
       61 tcgactggat ccggtaccaa ggtcgggcag gaagagggcc tatttcccat gattccttca
      121 tatttgcata tacgatacaa ggctgttaga gagataatta gaattaattt gactgtaaac
      181 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
      241 gttttaaaat tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc
      301 gatttcttgg ctttatatat cttgtggaaa ggacgaaaca ccattaagta ctagactcat
      361 ggtgtctttg tactctggta ccagaagcta caaagataag gcttcatgcc gaaatcaaca
      421 ccctgtcatt ttatggcagg gtgttttttt tactagttat taatagtaat caattacggg
      481 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      541 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      601 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      661 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      721 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      781 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
      841 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
      901 attattttgt gcagcgatgg gggcgggggg ggggggggsg cgcgccrggg gggggggggg
      961 gggggggggg gggggggggg gsgrggsggr grggkgsggc ggcagccaat cagagcggcg
     1021 cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa
     1081 gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg
     1141 cctcgcgccg cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg
     1201 acggcccttc tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc
     1261 tgtggctgcg tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct
     1321 cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg
     1381 gcggctgtga gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg
     1441 ggagcgcggc cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct
     1501 gcgtgcgggg tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc
     1561 aaccccccct gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc
     1621 tccgtacggg gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg
     1681 tgccgggcgg ggcggggccg ccycgggccg gggrgggctc gggggrgggg cgcggcggcc
     1741 cccggagcgc cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat
     1801 cgtgcgagag ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga
     1861 ggcgccgccg caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg
     1921 aaatgggcgg ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc
     1981 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
     2041 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
     2101 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
     2161 aattctgcag tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tgggcgcccc
     2221 aaagaagaag cggaaggtcg gtatccacgg agtcccagca gccagcgacc tggtgctggg
     2281 cctggacatc ggcatcggca gcgtgggcgt gggcatcctg aacaaggtga ccggcgagat
     2341 catccacaag aacagtcgca tcttccctgc tgctcaggct gagaacaacc tggtgcgccg
     2401 caccaaccgc cagggtcgcc ggcttgctcg ccgcaagaag caccggcgcg tgcgcctgaa
     2461 ccgcctgttc gaggagagcg gcctgatcac cgacttcacc aagatcagca tcaacctgaa
     2521 cccctaccag ctgcgcgtga agggcctgac cgacgagctg agcaacgagg agctgttcat
     2581 cgccctgaag aacatggtga agcaccgcgg catcagctac ctggacgacg ccagcgacga
     2641 cggcaacagc agcgtgggcg actacgccca gatcgtgaag gagaacagca agcagctgga
     2701 gaccaagacc cccggccaga tccagctgga gcgctaccag acctacggcc agctgcgcgg
     2761 cgacttcacc gtggagaagg acggcaagaa gcaccgcctg atcaacgtgt tccccaccag
     2821 cgcctaccgc agcgaggccc tgcgcatcct gcagacccag caggagttca acccccagat
     2881 caccgacgag ttcatcaacc gctacctgga gatcctgacc ggcaagcgca agtactacca
     2941 cggccccggc aacgagaaga gccgcaccga ctacggccgc taccgcacca gcggcgagac
     3001 cctggacaac atcttcggca tcctgatcgg caagtgcacc ttctaccccg acgagttccg
     3061 cgccgccaag gccagctaca ccgcccagga gttcaatctg ctgaacgacc tgaacaacct
     3121 gaccgtgccc accgagacca agaagctgag caaggagcag aagaaccaga tcatcaacta
     3181 cgtgaagaac gagaaggcta tgggccccgc caagctgttc aagtacatcg ccaagctgct
     3241 gagctgcgac gtggccgaca tcaagggcta ccgcatcgac aagagcggca aggccgagat
     3301 ccacaccttc gaggcctacc gcaagatgaa gaccctggag accctggaca tcgagcagat
     3361 ggaccgagag accctggaca agctggccta cgtgctgacc ctgaacaccg agcgcgaggg
     3421 catccaggag gccctggagc acgagttcgc cgacggcagc ttcagccaga aacaggtgga
     3481 cgagctggtg cagttccgca aggccaacag cagcatcttc ggcaagggct ggcacaactt
     3541 cagcgtgaag ctgatgatgg agctgatccc cgagctgtac gagaccagcg aggagcagat
     3601 gaccatcctg acccgcctgg gcaagcagaa gaccaccagc agcagcaaca agaccaagta
     3661 catcgacgag aagctgctga ccgaggagat ctacaacccc gtggtggcca agagcgtgcg
     3721 ccaggccatc aagatcgtga acgccgccat caaggagtac ggcgacttcg acaacatcgt
     3781 gatcgagatg gcccgcgaga ccaacgagga cgacgagaag aaggccatcc agaagatcca
     3841 gaaggccaac aaggacgaga aggacgccgc catgctgaag gccgccaacc agtacaacgg
     3901 caaggccgag ctgccccaca gcgtgttcca cggccacaag cagctggcca ccaagatccg
     3961 cctgtggcac cagcagggcg agcgctgcct gtacaccggc aagaccatca gcatccacga
     4021 cctgatcaac aacagcaacc agttcgaggt ggaccacatc ctgcccctga gcatcacctt
     4081 cgacgacagc ctggccaaca aggtgctggt gtacgccacc gccaaccagg agaagggcca
     4141 gcgcaccccc taccaggccc tggacagcat ggacgacgcc tggagcttcc gcgagctgaa
     4201 ggccttcgtg cgcgagagca agaccctgag caacaagaag aaggagtatc tgctgaccga
     4261 ggaggacatc agcaagttcg acgtgcgcaa gaagttcatc gagcgcaacc tggtggacac
     4321 ccgctacgcc agccgcgtgg tgctgaacgc cctgcaggag cacttccgcg cccacaagat
     4381 cgacaccaag gtgagcgtgg tgcgcggcca gttcaccagc cagctgcgcc gccactgggg
     4441 catcgagaag acccgcgaca cctaccacca ccacgccgtg gacgccctga tcattgcggc
     4501 ttctagccag ctgaacctgt ggaagaagca gaagaacacc ctggtgagct acagcgagga
     4561 ccagctgctg gacatcgaga ccggcgagct gatcagcgac gacgagtaca aggagagcgt
     4621 gttcaaggcc ccctaccagc acttcgtgga caccctgaag agcaaggagt tcgaggacag
     4681 catcctgttc agctaccagg tggacagcaa gttcaaccgc aagatcagcg acgccaccat
     4741 ctacgccacc cgccaggcca aggtgggcaa ggacaaggcc gacgagacct acgtgctggg
     4801 caagatcaag gacatctaca cccaggacgg ctacgacgcc ttcatgaaga tctacaagaa
     4861 ggacaagagc aagttcctga tgtaccgcca cgacccccag accttcgaga aggtgatcga
     4921 gcccatcctg gagaactacc ccaacaagca gatcaacgag aaaggcaagg aggtgccctg
     4981 caaccccttc ctgaagtaca aggaggagca cggctacatc cgcaagtaca gcaagaaggg
     5041 caacggcccc gagatcaaga gcctgaagta ctacgacagc aagctgggca accacatcga
     5101 catcaccccc aaggacagca acaacaaggt ggtgctgcag agccttaagc cctggcgcac
     5161 cgacgtgtac ttcaacaagg ccaccggcaa gtacgagatc ctggggctga agtacgccga
     5221 tctgcagttt gagaaaggca caggcaccta caagatcagc caggagaagt acaacgacat
     5281 caagaagaag gagggcgtgg acagcgacag cgagttcaag ttcaccctgt acaagaacga
     5341 ccttctgctg gtgaaggaca ccgagaccaa ggagcaacag ctgttccgct tcctgagccg
     5401 caccctgccc aagcagaagc actacgtgga gctgaagccc tacgacaagc agaagttcga
     5461 gggcggcgag gccctgatca aggtgctggg caacgtggcc aacggcggcc agtgcatcaa
     5521 gggcctggcc aagagcaaca tcagcatcta caaggtgcgc accgacgtgc tgggcaacca
     5581 gcacatcatc aagaacgagg gcgacaagcc caagttggac ttcagcaggg ctgaccccaa
     5641 gaagaagagg aaggtgggat cctgataaga attcgcggcc gcactcctca ggtgcaggct
     5701 gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat
     5761 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     5821 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     5881 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
     5941 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt
     6001 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     6061 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     6121 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     6181 gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg
     6241 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     6301 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     6361 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc
     6421 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     6481 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6541 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     6601 ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     6661 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     6721 tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg
     6781 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6841 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6901 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6961 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7021 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7081 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7141 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7201 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7261 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7321 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7381 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7441 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7501 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7561 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7621 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7681 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7741 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7801 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7861 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     7921 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     7981 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8041 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8101 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8161 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8221 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8281 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8341 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8401 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8461 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8521 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8581 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8641 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8701 aggggttccg cgcacatttc cccgaaaagt gccacctg
//
