LOCUS       sequence-422881-       11144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For plant prime editing in wheat plants or monocotyledons 
            protoplasts.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11144)
  AUTHORS   Ni P, Zhao Y, Zhou X, Liu Z, Huang Z, Ni Z, Sun Q, Zong Y
  TITLE     Efficient and versatile multiplex prime editing in hexaploid wheat.
  JOURNAL   Genome Biol. 2023 Jun 29;24(1):156. doi: 10.1186/s13059-023-02990-1.
  PUBMED    37386475
REFERENCE   2  (bases 1 to 11144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2023-06-29"; volume: "24(1):156. doi"; pages: " 
            10.1186/s13059-023-02990-1"
COMMENT     Sequence Label: ePPEmax*
FEATURES             Location/Qualifiers
     source          1..11144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2008..2034
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             2074..2094
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             6108..6116
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6418..6438
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8164..8184
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8244..8420
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(8974..8990)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8974..8990)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8987..9009)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8998..9014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9022..9028,9029..9046,9047..9052))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9067..9088
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9205..9222)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9376..9964)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9456..9475)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10135..10926,10927..10995))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10758..10777
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10996..11100)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc cccctctcta
     1021 ccttctctag atcggcgttc cggtccatgg ttagggcccg gtagttctac ttctgttcat
     1081 gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct agcgttcgta cacggatgcg
     1141 acctgtacgt cagacacgtt ctgattgcta acttgccagt gtttctcttt ggggaatcct
     1201 gggatggctc tagccgttcc gcagacggga tcgatttcat gatttttttt gtttcgttgc
     1261 atagggtttg gtttgccctt ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg
     1321 tcatcttttc atgctttttt ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt
     1381 tctagatcgg agtagaatta attctgtttc aaactacctg gtggatttat taattttgga
     1441 tctgtatgtg tgtgccatac atattcatag ttacgaattg aagatgatgg atggaaatat
     1501 cgatctagga taggtataca tgttgatgcg ggttttactg atgcatatac agagatgctt
     1561 tttgttcgct tggttgtgat gatgtggtgt ggttgggcgg tcgttcattc gttctagatc
     1621 ggagtagaat actgtttcaa actacctggt gtatttatta attttggaac tgtatgtgtg
     1681 tgtcatacat cttcatagtt acgagtttaa gatggatgga aatatcgatc taggataggt
     1741 atacatgttg atgtgggttt tactgatgca tatacatgat ggcatatgca gcatctattc
     1801 atatgctcta accttgagta cctatctatt ataataaaca agtatgtttt ataattattt
     1861 tgatcttgat atacttggat gatggcatat gcagcagcta tatgtggatt tttttagccc
     1921 tgccttcata cgctatttat ttgcttggta ctgtttcttt tgtcgatgct caccctgttg
     1981 tttggtgtta cttctgcaaa gcttatgcca gccgccaagc gcgtcaagct ggacggcgga
     2041 aagcgcaccg ccgacggatc cgagttcgag agccctaaga aaaagagaaa agtggacaag
     2101 aagtactcga tcggcctcga tattgggact aactctgttg gctgggccgt gatcaccgac
     2161 gagtacaagg tgccctcaaa gaagttcaag gtcctgggca acaccgatcg gcattccatc
     2221 aagaagaatc tcattggcgc tctcctgttc gacagcggcg agacggctga ggctacgcgg
     2281 ctcaagcgca ccgcccgcag gcggtacacg cgcaggaaga atcgcatctg ctacctgcag
     2341 gagattttct ccaacgagat ggcgaaggtt gacgattctt tcttccacag gctggaggag
     2401 tcattcctcg tggaggagga taagaagcac gagcggcatc caatcttcgg caacattgtc
     2461 gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa gaagctcgtg
     2521 gactccacag ataaggcgga cctccgcctg atctacctcg ctctggccca catgattaag
     2581 ttcaggggcc atttcctgat cgagggggat ctcaacccgg acaatagcga tgttgacaag
     2641 ctgttcatcc agctcgtgca gacgtacaac cagctcttcg aggagaaccc cattaatgcg
     2701 tcaggcgtcg acgcgaaggc tatcctgtcc gctaggctct cgaagtctcg gaagctcgag
     2761 aacctgatcg cccagctgcc gggcgagaag aagaacggcc tgttcgggaa tctcattgcg
     2821 ctcagcctgg ggctcacgcc caacttcaag tcgaatttcg atctcgctga ggacgccaag
     2881 ctgcagctct ccaaggacac atacgacgat gacctggata acctcctggc ccagatcggc
     2941 gatcagtacg cggacctgtt cctcgctgcc aagaatctgt cggacgccat cctcctgtct
     3001 gatattctca gggtgaacac cgagattacg aaggctccgc tctcagcctc catgatcaag
     3061 cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtcag gcagcagctc
     3121 cccgagaagt acaaggagat cttcttcgat cagtcgaaga acggctacgc tgggtacatt
     3181 gacggcgggg cctctcagga ggagttctac aagttcatca agccgattct ggagaagatg
     3241 gacggcacgg aggagctgct ggtgaagctc aagcgcgagg acctcctgag gaagcagcgg
     3301 acattcgata acggcagcat cccacaccag attcatctcg gggagctgca cgctatcctg
     3361 aggaggcagg aggacttcta ccctttcctc aaggataacc gcgagaagat cgagaagatt
     3421 ctgactttca ggatcccgta ctacgtcggc ccactcgcta ggggcaactc ccgcttcgct
     3481 tggatgaccc gcaagtcaga ggagacgatc acgccgtgga acttcgagga ggtggtcgac
     3541 aagggcgcta gcgctcagtc gttcatcgag aggatgacga atttcgacaa gaacctgcca
     3601 aatgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcacagt ctacaacgag
     3661 ctgactaagg tgaagtatgt gaccgagggc atgaggaagc cggctttcct gtctggggag
     3721 cagaagaagg ccatcgtgga cctcctgttc aagaccaacc ggaaggtcac ggttaagcag
     3781 ctcaaggagg actacttcaa gaagattgag tgcttcgatt cggtcgagat ctctggcgtt
     3841 gaggaccgct tcaacgcctc cctggggacc taccacgatc tcctgaagat cattaaggat
     3901 aaggacttcc tggacaacga ggagaatgag gatatcctcg aggacattgt gctgacactc
     3961 actctgttcg aggaccggga gatgatcgag gagcgcctga agacttacgc ccatctcttc
     4021 gatgacaagg tcatgaagca gctcaagagg aggaggtaca ccggctgggg gaggctgagc
     4081 aggaagctca tcaacggcat tcgggacaag cagtccggga agacgatcct cgacttcctg
     4141 aagagcgatg gcttcgcgaa ccgcaatttc atgcagctga ttcacgatga cagcctcaca
     4201 ttcaaggagg atatccagaa ggctcaggtg agcggccagg gggactcgct gcacgagcat
     4261 atcgcgaacc tcgctggctc gccagctatc aagaagggga ttctgcagac cgtgaaggtt
     4321 gtggacgagc tggtgaaggt catgggcagg cacaagcctg agaacatcgt cattgagatg
     4381 gcccgggaga atcagaccac gcagaagggc cagaagaact cacgcgagag gatgaagagg
     4441 atcgaggagg gcattaagga gctggggtcc cagatcctca aggagcaccc ggtggagaac
     4501 acgcagctgc agaatgagaa gctctacctg tactacctcc agaatggccg cgatatgtat
     4561 gtggaccagg agctggatat taacaggctc agcgattacg acgtcgatgc catcgttcca
     4621 cagtcattcc tgaaggatga ctccattgac aacaaggtcc tcaccaggtc ggacaagaac
     4681 cggggcaagt ctgataatgt tccttcagag gaggtcgtta agaagatgaa gaactactgg
     4741 cgccagctcc tgaatgccaa gctgatcacg cagcggaagt tcgataacct cacaaaggct
     4801 gagaggggcg ggctctctga gctggacaag gcgggcttca tcaagaggca gctggtcgag
     4861 acacggcaga tcactaagca cgttgcgcag attctcgact cacggatgaa cactaagtac
     4921 gatgagaatg acaagctgat ccgcgaggtg aaggtcatca ccctgaagtc aaagctcgtc
     4981 tccgacttca ggaaggattt ccagttctac aaggttcggg agatcaacaa ttaccaccat
     5041 gcccatgacg cgtacctgaa cgcggtggtc ggcacagctc tgatcaagaa gtacccaaag
     5101 ctcgagagcg agttcgtgta cggggactac aaggtttacg atgtgaggaa gatgatcgcc
     5161 aagtcggagc aggagattgg caaggctacc gccaagtact tcttctactc taacattatg
     5221 aatttcttca agacagagat cactctggcc aatggcgaga tccggaagcg ccccctcatc
     5281 gagacgaacg gcgagacggg ggagatcgtg tgggacaagg gcagggattt cgcgaccgtc
     5341 aggaaggttc tctccatgcc acaagtgaat atcgtcaaga agacagaggt ccagactggc
     5401 gggttctcta aggagtcaat tctgcctaag cggaacagcg acaagctcat cgcccgcaag
     5461 aaggactggg atccgaagaa gtacggcggg ttcgacagcc ccactgtggc ctactcggtc
     5521 ctggttgtgg cgaaggttga gaagggcaag tccaagaagc tcaagagcgt gaaggagctg
     5581 ctggggatca cgattatgga gcgctccagc ttcgagaaga acccgatcga tttcctggag
     5641 gcgaagggct acaaggaggt gaagaaggac ctgatcatta agctccccaa gtactcactc
     5701 ttcgagctgg agaacggcag gaagcggatg ctggcttccg ctggcgagct gcagaagggg
     5761 aacgagctgg ctctgccgtc caagtatgtg aacttcctct acctggcctc ccactacgag
     5821 aagctcaagg gcagccccga ggacaacgag cagaagcagc tgttcgtcga gcagcacaag
     5881 cattacctcg acgagatcat tgagcagatt tccgagttct ccaagcgcgt gatcctggcc
     5941 gacgcgaatc tggataaggt cctctccgcg tacaacaagc accgcgacaa gccaatcagg
     6001 gagcaggctg agaatatcat tcatctcttc accctgacga acctcggcgc ccctgctgct
     6061 ttcaagtact tcgacacaac tatcgatcgc aagaggtaca caagcactaa ggaggtcctg
     6121 gacgcgaccc tcatccacca gtcgattacc ggcctctacg agacgcgcat cgacctgtct
     6181 cagctcgggg gcgacgaatt ctccgggagc gagacgccag gcacctccga gtcggccacc
     6241 ccagaatctg ccacagtggt gtccggccaa aagcaggacc gccagggcgg agaacgcaga
     6301 aggtcccagc tcgataggga tcagtgtgcc tactgcaagg agaagggcca ctgggccaaa
     6361 gactgcccga aaaagccgcg cggcccacgc ggcccaaggc cacaaacatc cctccttcca
     6421 aagaagaagc ggaaggtgga gctcagcgga ggatcttccg gaggatctag cggctccgag
     6481 acaccaggaa catccgaaag cgctacacca gaatctagcg gaggctcttc cggaggatct
     6541 aggcctaccc tcaacatcga ggatgagtat cgcctccacg aaacctccaa agaaccggac
     6601 gtgtccctcg gcagcacatg gctcagcgac ttcccacaag cgtgggccga aaccggcggc
     6661 atgggcctcg ccgtccgcca agccccactc attatcccgc tgaaggcgac ctccacaccg
     6721 gtgtccatca agcagtaccc gatgagccaa gaggcgaggc tcgggattaa gccgcacatt
     6781 cagcgcctcc tcgatcaagg cattctcgtg ccgtgccaat ccccgtggaa tacaccactc
     6841 ctcccggtca aaaagccggg caccaacgac tatcgcccgg tccaagatct ccgcgaggtc
     6901 aacaagcgcg tggaagacat ccacccgacc gtcccgaacc cgtataatct gctctccggg
     6961 ctcccaccat cccaccagtg gtatacagtg ctggacctca aagacgcctt cttctgtctc
     7021 cgcctccacc caacaagcca gccgctcttc gccttcgagt ggcgcgaccc ggagatgggc
     7081 atctccggcc aactgacatg gacacgcctc ccgcaaggct tcaagaacag cccgacactc
     7141 ttcaacgagg cgctccatag ggacctcgcg gattttcgca tccagcatcc ggacctcatc
     7201 ctcctccagt atgtggatga tctcctcctc gccgcgacct ccgagctgga ttgtcaacaa
     7261 ggcacacgcg cgctcctcca aacactcggg aacctcggct atcgcgcgtc cgcgaaaaag
     7321 gcccaaatct gccagaagca agtgaagtac ctcgggtatc tgctcaagga aggccaacgc
     7381 tggctcaccg aagcgcgcaa agaaacagtg atggggcaac cgacaccgaa aacaccacgc
     7441 cagctgcgcg agtttctcgg caaagccggc ttctgtcgcc tcttcatccc gggctttgcc
     7501 gagatggccg cgccactcta cccactcacc aagccgggca cactgtttaa ctgggggccg
     7561 gatcagcaga aagcctacca agagatcaaa caagcgctcc tcaccgcccc agcgctcggg
     7621 ctcccagatc tcacaaagcc gttcgagctg ttcgtcgatg agaagcaagg ctacgcgaag
     7681 ggcgtgctca cacagaagct cggcccgtgg aggaggccag tggcctatct ctccaaaaaa
     7741 ctcgatccag tggccgccgg ctggccaccg tgtctgcgca tggtcgccgc gattgccgtg
     7801 ctcacaaagg atgccggcaa actcacaatg ggccagccgc tggtgatcct cgcgccacat
     7861 gccgtggaag ccctcgtcaa acagccgccg gataggtggc tctccaatgc gcgcatgacc
     7921 cattaccaag cgctcctcct cgacaccgat cgcgtccagt tcggcccagt ggtcgccctc
     7981 aatccggcga cactgctgcc actcccagag gagggcctcc aacacaactg tctggatatt
     8041 ctcgcggaag cgcatggcac aaggccagac ctcacagatc aaccgctcag cggcggcagc
     8101 aaaaggaccg ccgattccca acattccacc ccaccaaaaa ccaagaggaa ggtgggctct
     8161 ggcccaaaga agaagaggaa ggtctgagcg gccgcggtac gctgaaatca ccagtctctc
     8221 tctacaaatc tatctctctc tattttctcc ataaataatg tgtgagtagt ttcccgataa
     8281 gggaaattag ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt
     8341 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
     8401 ccagtactaa aatccagatc tcctaaagtc cctatagatc tttgtcgtga atataaacca
     8461 gacacgagac gactaaacct ggagcccaga cgccgttcga agctagaagt accgcttagg
     8521 caggaggccg ttagggaaaa gatgctaagg cagggttggt tacgttgact cccccgtagg
     8581 tttggtttaa atatgatgaa gtggacggaa ggaaggagga agacaaggaa ggataaggtt
     8641 gcaggccctg tgcaaggtaa gaagatggaa atttgataga ggtacgctac tatacttata
     8701 ctatacgcta agggaatgct tgtatttata ccctataccc cctaataacc ccttatcaat
     8761 ttaagaaata atccgcataa gcccccgctt aaaaattggt atcagagcca tgaataggtc
     8821 tatgaccaaa actcaagagg ataaaacctc accaaaatac gaaagagttc ttaactctaa
     8881 agataaaaga tctttcaaga tcaaaactag ttccctcaca ccggtgacgg ggatcgcatg
     8941 cgatatctcg agatctagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     9001 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     9061 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     9121 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     9181 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     9241 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9301 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9361 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9421 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9481 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9541 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9601 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9661 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9721 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9781 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     9841 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9901 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9961 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
    10021 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    10081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    10141 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    10201 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    10261 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10321 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10381 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10441 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10501 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10561 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10621 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10681 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10741 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10801 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10861 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10921 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10981 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    11041 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    11101 cacatttccc cgaaaagtgc cacctgccag tgccaagcta attc
//
